Optimization of temperature, moisture content and inoculum size in solid state fermentation to enhance mannanase production by Aspergillus terreus SUK-1 using RSM

Jahwarhar Izuan Abdul Rashid, Noraini Samat, Wan Mohtar Wan Yusoff

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Optimization of three parameters, temperature (25-35°C), moisture content (40% (w/v)-60% (w/v) and inoculum sizes (5% (w/v)-l 5% (w/v) were investigated and optimized by Response Surface Methodology (RSM) for optimal mannanase production by Aspergillus terreus SUK-1. A second order polynomial equation was fitted and the optimum condition was established. The result showed that the moisture content was a critical factor in terms of its effect on mannanase. The optimum condition for mannanase production was predicted at 42.86% (w/v) initial moisture (31 °C) temperature and 5.5% (w/v) inoculum size. The predicted optimal parameter were tested in the laboratory and the mannanase activity 45.12 IU mL -1 were recorded to be closed to the predicted value (44.80 IU mL -1). Under the optimized SSF condition (31 °C, 42.86% moisture content (w/v) and 5.5% inoculum size (w/v)), the maximum mannanase production was to prevail about 45.12 IUmL -1 compare to before optimized (30°C, 50% moisture content (w/v) and 10% inoculum size (w/v)) was only 34.42 IU mL -1.

Original languageEnglish
Pages (from-to)533-539
Number of pages7
JournalPakistan Journal of Biological Sciences
Volume14
Issue number9
DOIs
Publication statusPublished - 2011

Fingerprint

Aspergillus terreus
beta-mannosidase
solid state fermentation
response surface methodology
inoculum
water content
temperature

Keywords

  • Aspergillus terreus SUK-1
  • Mannanase
  • Palm kernel cake
  • Response surface methodology(RSM)
  • Solid state fermentation

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

@article{4bc798cf84af42e4b0de625df742a6f9,
title = "Optimization of temperature, moisture content and inoculum size in solid state fermentation to enhance mannanase production by Aspergillus terreus SUK-1 using RSM",
abstract = "Optimization of three parameters, temperature (25-35°C), moisture content (40{\%} (w/v)-60{\%} (w/v) and inoculum sizes (5{\%} (w/v)-l 5{\%} (w/v) were investigated and optimized by Response Surface Methodology (RSM) for optimal mannanase production by Aspergillus terreus SUK-1. A second order polynomial equation was fitted and the optimum condition was established. The result showed that the moisture content was a critical factor in terms of its effect on mannanase. The optimum condition for mannanase production was predicted at 42.86{\%} (w/v) initial moisture (31 °C) temperature and 5.5{\%} (w/v) inoculum size. The predicted optimal parameter were tested in the laboratory and the mannanase activity 45.12 IU mL -1 were recorded to be closed to the predicted value (44.80 IU mL -1). Under the optimized SSF condition (31 °C, 42.86{\%} moisture content (w/v) and 5.5{\%} inoculum size (w/v)), the maximum mannanase production was to prevail about 45.12 IUmL -1 compare to before optimized (30°C, 50{\%} moisture content (w/v) and 10{\%} inoculum size (w/v)) was only 34.42 IU mL -1.",
keywords = "Aspergillus terreus SUK-1, Mannanase, Palm kernel cake, Response surface methodology(RSM), Solid state fermentation",
author = "Rashid, {Jahwarhar Izuan Abdul} and Noraini Samat and {Wan Yusoff}, {Wan Mohtar}",
year = "2011",
doi = "10.3923/pjbs.2011.533.539",
language = "English",
volume = "14",
pages = "533--539",
journal = "Pakistan Journal of Biological Sciences",
issn = "1028-8880",
publisher = "Asian Network for Scientific Information",
number = "9",

}

TY - JOUR

T1 - Optimization of temperature, moisture content and inoculum size in solid state fermentation to enhance mannanase production by Aspergillus terreus SUK-1 using RSM

AU - Rashid, Jahwarhar Izuan Abdul

AU - Samat, Noraini

AU - Wan Yusoff, Wan Mohtar

PY - 2011

Y1 - 2011

N2 - Optimization of three parameters, temperature (25-35°C), moisture content (40% (w/v)-60% (w/v) and inoculum sizes (5% (w/v)-l 5% (w/v) were investigated and optimized by Response Surface Methodology (RSM) for optimal mannanase production by Aspergillus terreus SUK-1. A second order polynomial equation was fitted and the optimum condition was established. The result showed that the moisture content was a critical factor in terms of its effect on mannanase. The optimum condition for mannanase production was predicted at 42.86% (w/v) initial moisture (31 °C) temperature and 5.5% (w/v) inoculum size. The predicted optimal parameter were tested in the laboratory and the mannanase activity 45.12 IU mL -1 were recorded to be closed to the predicted value (44.80 IU mL -1). Under the optimized SSF condition (31 °C, 42.86% moisture content (w/v) and 5.5% inoculum size (w/v)), the maximum mannanase production was to prevail about 45.12 IUmL -1 compare to before optimized (30°C, 50% moisture content (w/v) and 10% inoculum size (w/v)) was only 34.42 IU mL -1.

AB - Optimization of three parameters, temperature (25-35°C), moisture content (40% (w/v)-60% (w/v) and inoculum sizes (5% (w/v)-l 5% (w/v) were investigated and optimized by Response Surface Methodology (RSM) for optimal mannanase production by Aspergillus terreus SUK-1. A second order polynomial equation was fitted and the optimum condition was established. The result showed that the moisture content was a critical factor in terms of its effect on mannanase. The optimum condition for mannanase production was predicted at 42.86% (w/v) initial moisture (31 °C) temperature and 5.5% (w/v) inoculum size. The predicted optimal parameter were tested in the laboratory and the mannanase activity 45.12 IU mL -1 were recorded to be closed to the predicted value (44.80 IU mL -1). Under the optimized SSF condition (31 °C, 42.86% moisture content (w/v) and 5.5% inoculum size (w/v)), the maximum mannanase production was to prevail about 45.12 IUmL -1 compare to before optimized (30°C, 50% moisture content (w/v) and 10% inoculum size (w/v)) was only 34.42 IU mL -1.

KW - Aspergillus terreus SUK-1

KW - Mannanase

KW - Palm kernel cake

KW - Response surface methodology(RSM)

KW - Solid state fermentation

UR - http://www.scopus.com/inward/record.url?scp=80052575266&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052575266&partnerID=8YFLogxK

U2 - 10.3923/pjbs.2011.533.539

DO - 10.3923/pjbs.2011.533.539

M3 - Article

VL - 14

SP - 533

EP - 539

JO - Pakistan Journal of Biological Sciences

JF - Pakistan Journal of Biological Sciences

SN - 1028-8880

IS - 9

ER -