Optimization of submerged culture conditions for the production of mycelial biomass and exopolysaccharides from lignosus rhinocerus

Wei Hong Lai, Saadiah Mohd Salleh, Fauzi Daud, Zamri Zainal, Abas Mazni Othman, Norihan Mohd Saleh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Tiger's Milk mushroom (Lignosus rhinocerus) is a highly priced medicinal mushroom utilized in traditional medicine to treat various diseases. However, due to insufficient wild L. rhinocerus, submerged culture conditions and nutritional requirements for the production of mycelial biomass and exopolysaccharide (EPS) from L. rhinocerus were studied using one-factor-at-a-time and orthogonal matrix method in shake flask culture. The optimal pH and temperature for ideal production of mycelial biomass and EPS were found to be at pH6 and 25°C, respectively. The optimal compositions for mycelial biomass production were 80 g/L of glucose, 4 g/L of potassium nitrate, 0.4 g/L of FeSO4.7H2O and 0.1 g/L of CaCI2. Subsequently, the optimal compositions for EPS production were 80 g/L of glucose, 4 g/L of potassium nitrate, 1.4 g/L of FeSO4.7H2O and 1.1 g/L of CaCI2. The maximum mycelial biomass and EPS concentrations achieved in a 1.5 L stirred-tank bioreactor were 6.3788 g/L and 1.2 g/L, respectively. Mycelial biomass production was about 3 times higher than that at the basal medium. However, EPS production indicated no significant difference at the basal medium. In addition, the concentrations for α-amylase, β-amylase, cellulase and invertase in optimal medium were 2.87, 1.07, 3.0 and 3.0 mg/mL, respectively. Current findings suggest that the production of mycelial biomass and EPS of L. rhinocerus can be enhanced dramatically by controlling the culture conditions and modifying the medium's composition.

Original languageEnglish
Pages (from-to)73-80
Number of pages8
JournalSains Malaysiana
Volume43
Issue number1
Publication statusPublished - Jan 2014

Fingerprint

biomass
mushroom
glucose
potassium
nitrate
traditional medicine
nutritional requirement
bioreactor
milk
matrix
temperature

Keywords

  • Exopolysaccharides
  • Lignosus rhinoceros
  • Mycelial biomass
  • Orthogonal matrix method
  • Submerged culture

ASJC Scopus subject areas

  • General

Cite this

Optimization of submerged culture conditions for the production of mycelial biomass and exopolysaccharides from lignosus rhinocerus. / Lai, Wei Hong; Salleh, Saadiah Mohd; Daud, Fauzi; Zainal, Zamri; Othman, Abas Mazni; Saleh, Norihan Mohd.

In: Sains Malaysiana, Vol. 43, No. 1, 01.2014, p. 73-80.

Research output: Contribution to journalArticle

Lai, Wei Hong ; Salleh, Saadiah Mohd ; Daud, Fauzi ; Zainal, Zamri ; Othman, Abas Mazni ; Saleh, Norihan Mohd. / Optimization of submerged culture conditions for the production of mycelial biomass and exopolysaccharides from lignosus rhinocerus. In: Sains Malaysiana. 2014 ; Vol. 43, No. 1. pp. 73-80.
@article{13e655bd040a412999ba01ec7c8db344,
title = "Optimization of submerged culture conditions for the production of mycelial biomass and exopolysaccharides from lignosus rhinocerus",
abstract = "Tiger's Milk mushroom (Lignosus rhinocerus) is a highly priced medicinal mushroom utilized in traditional medicine to treat various diseases. However, due to insufficient wild L. rhinocerus, submerged culture conditions and nutritional requirements for the production of mycelial biomass and exopolysaccharide (EPS) from L. rhinocerus were studied using one-factor-at-a-time and orthogonal matrix method in shake flask culture. The optimal pH and temperature for ideal production of mycelial biomass and EPS were found to be at pH6 and 25°C, respectively. The optimal compositions for mycelial biomass production were 80 g/L of glucose, 4 g/L of potassium nitrate, 0.4 g/L of FeSO4.7H2O and 0.1 g/L of CaCI2. Subsequently, the optimal compositions for EPS production were 80 g/L of glucose, 4 g/L of potassium nitrate, 1.4 g/L of FeSO4.7H2O and 1.1 g/L of CaCI2. The maximum mycelial biomass and EPS concentrations achieved in a 1.5 L stirred-tank bioreactor were 6.3788 g/L and 1.2 g/L, respectively. Mycelial biomass production was about 3 times higher than that at the basal medium. However, EPS production indicated no significant difference at the basal medium. In addition, the concentrations for α-amylase, β-amylase, cellulase and invertase in optimal medium were 2.87, 1.07, 3.0 and 3.0 mg/mL, respectively. Current findings suggest that the production of mycelial biomass and EPS of L. rhinocerus can be enhanced dramatically by controlling the culture conditions and modifying the medium's composition.",
keywords = "Exopolysaccharides, Lignosus rhinoceros, Mycelial biomass, Orthogonal matrix method, Submerged culture",
author = "Lai, {Wei Hong} and Salleh, {Saadiah Mohd} and Fauzi Daud and Zamri Zainal and Othman, {Abas Mazni} and Saleh, {Norihan Mohd}",
year = "2014",
month = "1",
language = "English",
volume = "43",
pages = "73--80",
journal = "Sains Malaysiana",
issn = "0126-6039",
publisher = "Penerbit Universiti Kebangsaan Malaysia",
number = "1",

}

TY - JOUR

T1 - Optimization of submerged culture conditions for the production of mycelial biomass and exopolysaccharides from lignosus rhinocerus

AU - Lai, Wei Hong

AU - Salleh, Saadiah Mohd

AU - Daud, Fauzi

AU - Zainal, Zamri

AU - Othman, Abas Mazni

AU - Saleh, Norihan Mohd

PY - 2014/1

Y1 - 2014/1

N2 - Tiger's Milk mushroom (Lignosus rhinocerus) is a highly priced medicinal mushroom utilized in traditional medicine to treat various diseases. However, due to insufficient wild L. rhinocerus, submerged culture conditions and nutritional requirements for the production of mycelial biomass and exopolysaccharide (EPS) from L. rhinocerus were studied using one-factor-at-a-time and orthogonal matrix method in shake flask culture. The optimal pH and temperature for ideal production of mycelial biomass and EPS were found to be at pH6 and 25°C, respectively. The optimal compositions for mycelial biomass production were 80 g/L of glucose, 4 g/L of potassium nitrate, 0.4 g/L of FeSO4.7H2O and 0.1 g/L of CaCI2. Subsequently, the optimal compositions for EPS production were 80 g/L of glucose, 4 g/L of potassium nitrate, 1.4 g/L of FeSO4.7H2O and 1.1 g/L of CaCI2. The maximum mycelial biomass and EPS concentrations achieved in a 1.5 L stirred-tank bioreactor were 6.3788 g/L and 1.2 g/L, respectively. Mycelial biomass production was about 3 times higher than that at the basal medium. However, EPS production indicated no significant difference at the basal medium. In addition, the concentrations for α-amylase, β-amylase, cellulase and invertase in optimal medium were 2.87, 1.07, 3.0 and 3.0 mg/mL, respectively. Current findings suggest that the production of mycelial biomass and EPS of L. rhinocerus can be enhanced dramatically by controlling the culture conditions and modifying the medium's composition.

AB - Tiger's Milk mushroom (Lignosus rhinocerus) is a highly priced medicinal mushroom utilized in traditional medicine to treat various diseases. However, due to insufficient wild L. rhinocerus, submerged culture conditions and nutritional requirements for the production of mycelial biomass and exopolysaccharide (EPS) from L. rhinocerus were studied using one-factor-at-a-time and orthogonal matrix method in shake flask culture. The optimal pH and temperature for ideal production of mycelial biomass and EPS were found to be at pH6 and 25°C, respectively. The optimal compositions for mycelial biomass production were 80 g/L of glucose, 4 g/L of potassium nitrate, 0.4 g/L of FeSO4.7H2O and 0.1 g/L of CaCI2. Subsequently, the optimal compositions for EPS production were 80 g/L of glucose, 4 g/L of potassium nitrate, 1.4 g/L of FeSO4.7H2O and 1.1 g/L of CaCI2. The maximum mycelial biomass and EPS concentrations achieved in a 1.5 L stirred-tank bioreactor were 6.3788 g/L and 1.2 g/L, respectively. Mycelial biomass production was about 3 times higher than that at the basal medium. However, EPS production indicated no significant difference at the basal medium. In addition, the concentrations for α-amylase, β-amylase, cellulase and invertase in optimal medium were 2.87, 1.07, 3.0 and 3.0 mg/mL, respectively. Current findings suggest that the production of mycelial biomass and EPS of L. rhinocerus can be enhanced dramatically by controlling the culture conditions and modifying the medium's composition.

KW - Exopolysaccharides

KW - Lignosus rhinoceros

KW - Mycelial biomass

KW - Orthogonal matrix method

KW - Submerged culture

UR - http://www.scopus.com/inward/record.url?scp=84892574423&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84892574423&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84892574423

VL - 43

SP - 73

EP - 80

JO - Sains Malaysiana

JF - Sains Malaysiana

SN - 0126-6039

IS - 1

ER -