Abstract
Tiger's Milk mushroom (Lignosus rhinocerus) is a highly priced medicinal mushroom utilized in traditional medicine to treat various diseases. However, due to insufficient wild L. rhinocerus, submerged culture conditions and nutritional requirements for the production of mycelial biomass and exopolysaccharide (EPS) from L. rhinocerus were studied using one-factor-at-a-time and orthogonal matrix method in shake flask culture. The optimal pH and temperature for ideal production of mycelial biomass and EPS were found to be at pH6 and 25°C, respectively. The optimal compositions for mycelial biomass production were 80 g/L of glucose, 4 g/L of potassium nitrate, 0.4 g/L of FeSO4.7H2O and 0.1 g/L of CaCI2. Subsequently, the optimal compositions for EPS production were 80 g/L of glucose, 4 g/L of potassium nitrate, 1.4 g/L of FeSO4.7H2O and 1.1 g/L of CaCI2. The maximum mycelial biomass and EPS concentrations achieved in a 1.5 L stirred-tank bioreactor were 6.3788 g/L and 1.2 g/L, respectively. Mycelial biomass production was about 3 times higher than that at the basal medium. However, EPS production indicated no significant difference at the basal medium. In addition, the concentrations for α-amylase, β-amylase, cellulase and invertase in optimal medium were 2.87, 1.07, 3.0 and 3.0 mg/mL, respectively. Current findings suggest that the production of mycelial biomass and EPS of L. rhinocerus can be enhanced dramatically by controlling the culture conditions and modifying the medium's composition.
| Original language | English |
|---|---|
| Pages (from-to) | 73-80 |
| Number of pages | 8 |
| Journal | Sains Malaysiana |
| Volume | 43 |
| Issue number | 1 |
| Publication status | Published - Jan 2014 |
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Keywords
- Exopolysaccharides
- Lignosus rhinoceros
- Mycelial biomass
- Orthogonal matrix method
- Submerged culture
ASJC Scopus subject areas
- General
Cite this
Optimization of submerged culture conditions for the production of mycelial biomass and exopolysaccharides from lignosus rhinocerus. / Lai, Wei Hong; Salleh, Saadiah Mohd; Daud, Fauzi; Zainal, Zamri; Othman, Abas Mazni; Saleh, Norihan Mohd.
In: Sains Malaysiana, Vol. 43, No. 1, 01.2014, p. 73-80.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Optimization of submerged culture conditions for the production of mycelial biomass and exopolysaccharides from lignosus rhinocerus
AU - Lai, Wei Hong
AU - Salleh, Saadiah Mohd
AU - Daud, Fauzi
AU - Zainal, Zamri
AU - Othman, Abas Mazni
AU - Saleh, Norihan Mohd
PY - 2014/1
Y1 - 2014/1
N2 - Tiger's Milk mushroom (Lignosus rhinocerus) is a highly priced medicinal mushroom utilized in traditional medicine to treat various diseases. However, due to insufficient wild L. rhinocerus, submerged culture conditions and nutritional requirements for the production of mycelial biomass and exopolysaccharide (EPS) from L. rhinocerus were studied using one-factor-at-a-time and orthogonal matrix method in shake flask culture. The optimal pH and temperature for ideal production of mycelial biomass and EPS were found to be at pH6 and 25°C, respectively. The optimal compositions for mycelial biomass production were 80 g/L of glucose, 4 g/L of potassium nitrate, 0.4 g/L of FeSO4.7H2O and 0.1 g/L of CaCI2. Subsequently, the optimal compositions for EPS production were 80 g/L of glucose, 4 g/L of potassium nitrate, 1.4 g/L of FeSO4.7H2O and 1.1 g/L of CaCI2. The maximum mycelial biomass and EPS concentrations achieved in a 1.5 L stirred-tank bioreactor were 6.3788 g/L and 1.2 g/L, respectively. Mycelial biomass production was about 3 times higher than that at the basal medium. However, EPS production indicated no significant difference at the basal medium. In addition, the concentrations for α-amylase, β-amylase, cellulase and invertase in optimal medium were 2.87, 1.07, 3.0 and 3.0 mg/mL, respectively. Current findings suggest that the production of mycelial biomass and EPS of L. rhinocerus can be enhanced dramatically by controlling the culture conditions and modifying the medium's composition.
AB - Tiger's Milk mushroom (Lignosus rhinocerus) is a highly priced medicinal mushroom utilized in traditional medicine to treat various diseases. However, due to insufficient wild L. rhinocerus, submerged culture conditions and nutritional requirements for the production of mycelial biomass and exopolysaccharide (EPS) from L. rhinocerus were studied using one-factor-at-a-time and orthogonal matrix method in shake flask culture. The optimal pH and temperature for ideal production of mycelial biomass and EPS were found to be at pH6 and 25°C, respectively. The optimal compositions for mycelial biomass production were 80 g/L of glucose, 4 g/L of potassium nitrate, 0.4 g/L of FeSO4.7H2O and 0.1 g/L of CaCI2. Subsequently, the optimal compositions for EPS production were 80 g/L of glucose, 4 g/L of potassium nitrate, 1.4 g/L of FeSO4.7H2O and 1.1 g/L of CaCI2. The maximum mycelial biomass and EPS concentrations achieved in a 1.5 L stirred-tank bioreactor were 6.3788 g/L and 1.2 g/L, respectively. Mycelial biomass production was about 3 times higher than that at the basal medium. However, EPS production indicated no significant difference at the basal medium. In addition, the concentrations for α-amylase, β-amylase, cellulase and invertase in optimal medium were 2.87, 1.07, 3.0 and 3.0 mg/mL, respectively. Current findings suggest that the production of mycelial biomass and EPS of L. rhinocerus can be enhanced dramatically by controlling the culture conditions and modifying the medium's composition.
KW - Exopolysaccharides
KW - Lignosus rhinoceros
KW - Mycelial biomass
KW - Orthogonal matrix method
KW - Submerged culture
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UR - http://www.scopus.com/inward/citedby.url?scp=84892574423&partnerID=8YFLogxK
M3 - Article
VL - 43
SP - 73
EP - 80
JO - Sains Malaysiana
JF - Sains Malaysiana
SN - 0126-6039
IS - 1
ER -