Optimization of protease extraction from horse mango (Mangifera foetida Lour) kernels by a response surface methodology

Mohammad Norazmi Ahmad, Siew Ling Liew, Mohd. Ambar Yarmo, Mamot Said

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Protease is one of the most important industrial enzymes with a multitude of applications in both food and non-food sectors. Although most commercial proteases are microbial proteases, the potential of nonconventional protease sources, especially plants, should not be overlooked. In this study, horse mango (Mangifera foetida Lour) fruit, known to produce latex with a blistering effect upon contact with human skin, was chosen as a source of protease, and the effect of the extraction process on its protease activity evaluated. The crude enzyme was extracted from the kernels and extraction was optimized by a response surface methodology (RSM) using a central composite rotatable design (CCRD). The variables studied were pH (x1), CaCl2 (x2), Triton X-100 (x3), and 1,4-dithryeitol (x4). The results obtained indicate that the quadratic model is significant for all the variables tested. Based on the RSM model generated, optimal extraction conditions were obtained at pH 6.0, 8.16mM CaCl2, 5.0% Triton X-100, and 10.0mM DTT, and the estimated response was 95.5% (w/w). Verification test results showed that the difference between the calculated and the experimental protease activity value was only 2%. Based on the tvalue, the effects of the variables arranged in ascending order of strength were CaCl2 < pH < DTT < Triton X-100.

Original languageEnglish
Pages (from-to)1438-1444
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume76
Issue number8
DOIs
Publication statusPublished - 2012

Fingerprint

Mangifera
Peptide Hydrolases
Octoxynol
Latex
Enzymes
Fruits
Fruit
Skin
Food
Composite materials

Keywords

  • Central composite rotatable design
  • Mangifera foetida Lour
  • Optimization
  • Protease
  • Response surface methodology

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Optimization of protease extraction from horse mango (Mangifera foetida Lour) kernels by a response surface methodology. / Ahmad, Mohammad Norazmi; Liew, Siew Ling; Yarmo, Mohd. Ambar; Said, Mamot.

In: Bioscience, Biotechnology and Biochemistry, Vol. 76, No. 8, 2012, p. 1438-1444.

Research output: Contribution to journalArticle

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