Optimization of pretreatments for the hydrolysis of oil palm empty fruit bunch fiber (EFBF) using enzyme mixtures

Osman Hassan, Tang Pei Ling, Mohamad Yusof Maskat, Rosli Md Illias, Khairiah Badri, Jamaliah Md Jahim, Nor Muhammad Mahadi

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

A study was conducted to optimize sugar production from oil palm empty fruit bunch fiber (EFBF). Three different pretreatments were applied to EFBF, namely steam, steam with 5% sodium hydroxide (steam+5% NaOH) and steam with 5% acetic acid (steam+5% acetic acid). The hydrolyzability of the pretreated EFBF was determined using cellulolytic enzyme mixture comprising of Celluclast 1.5L (C), Viscozyme L (V) and Novozyme 188 (N). The different pretreatments showed varying degree of severities to the morphology of the fiber. Steam+5% acetic acid pretreatment was found to cause the most severe changes to the EFBF surface. The optimum combinations of the enzymes for EFBF degradation, using a fixed parameters, were determined using Simplex Lattice mixture design. For untreated, steam pretreated, steam+5% NaOH, steam+5% acetic acid EFBFs, the optimum combinations enzyme were 0.49C: 0.45V: 0.06N, 0.88C: 0.12N, 0.78C: 0.03V: 0.2N and 0.90C: 0.03V: 0.07N respectively. Surface morphology of EFBF appears to be the major contributing factor that affects efficiency of enzymatic hydrolysis. Among these four samples, steam+5% acetic acid pretreated EFBF gave the highest yield of sugars (44.36% xylose; 52.03% glucose). Applying the best enzymatic combinations, saccharification of pretreated samples was optimized using RSM. saccharification of the steam+5% acetic acid pretreated EFBF was able to yield 99.90±10.92mgg-1 xylose and 281.77±28.00mgg-1 glucose at 2.51% enzyme loading (with total protein loading 22.1mg per gram EFBF) in 4.55% EFBF for 35.08h of reaction. Overall, through the combination of steam+5% acetic acid pretreatment, enzyme mixture optimization and optimization of enzymatic saccharification, EFBF has the potential to produce substantial amount of sugars (62.36% xylose; 81.84% glucose).

Original languageEnglish
Pages (from-to)137-146
Number of pages10
JournalBiomass and Bioenergy
Volume56
DOIs
Publication statusPublished - Sep 2013

Fingerprint

Palm oil
Elaeis guineensis
Fruits
steam
hydrolysis
Hydrolysis
Steam
Enzymes
pretreatment
fruit
enzyme
fruits
Fibers
oil
Acetic acid
acetic acid
enzymes
Saccharification
saccharification
Xylose

Keywords

  • Alkaline
  • Dilute acid
  • Enzymatic hydrolysis
  • Oil palm empty fruit bunch fiber (EFBF)
  • Pretreatments
  • Steam

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Forestry
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Optimization of pretreatments for the hydrolysis of oil palm empty fruit bunch fiber (EFBF) using enzyme mixtures. / Hassan, Osman; Ling, Tang Pei; Maskat, Mohamad Yusof; Illias, Rosli Md; Badri, Khairiah; Md Jahim, Jamaliah; Mahadi, Nor Muhammad.

In: Biomass and Bioenergy, Vol. 56, 09.2013, p. 137-146.

Research output: Contribution to journalArticle

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abstract = "A study was conducted to optimize sugar production from oil palm empty fruit bunch fiber (EFBF). Three different pretreatments were applied to EFBF, namely steam, steam with 5{\%} sodium hydroxide (steam+5{\%} NaOH) and steam with 5{\%} acetic acid (steam+5{\%} acetic acid). The hydrolyzability of the pretreated EFBF was determined using cellulolytic enzyme mixture comprising of Celluclast 1.5L (C), Viscozyme L (V) and Novozyme 188 (N). The different pretreatments showed varying degree of severities to the morphology of the fiber. Steam+5{\%} acetic acid pretreatment was found to cause the most severe changes to the EFBF surface. The optimum combinations of the enzymes for EFBF degradation, using a fixed parameters, were determined using Simplex Lattice mixture design. For untreated, steam pretreated, steam+5{\%} NaOH, steam+5{\%} acetic acid EFBFs, the optimum combinations enzyme were 0.49C: 0.45V: 0.06N, 0.88C: 0.12N, 0.78C: 0.03V: 0.2N and 0.90C: 0.03V: 0.07N respectively. Surface morphology of EFBF appears to be the major contributing factor that affects efficiency of enzymatic hydrolysis. Among these four samples, steam+5{\%} acetic acid pretreated EFBF gave the highest yield of sugars (44.36{\%} xylose; 52.03{\%} glucose). Applying the best enzymatic combinations, saccharification of pretreated samples was optimized using RSM. saccharification of the steam+5{\%} acetic acid pretreated EFBF was able to yield 99.90±10.92mgg-1 xylose and 281.77±28.00mgg-1 glucose at 2.51{\%} enzyme loading (with total protein loading 22.1mg per gram EFBF) in 4.55{\%} EFBF for 35.08h of reaction. Overall, through the combination of steam+5{\%} acetic acid pretreatment, enzyme mixture optimization and optimization of enzymatic saccharification, EFBF has the potential to produce substantial amount of sugars (62.36{\%} xylose; 81.84{\%} glucose).",
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AU - Badri, Khairiah

AU - Md Jahim, Jamaliah

AU - Mahadi, Nor Muhammad

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