Optimization of oven drying conditions for lycopene content and lipophilic antioxidant capacity in a by-product of the pink guava puree industry using response surface methodology

Kin Weng Kong, Amin Ismail, Chin Ping Tan, Nor Fadilah Rajab

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27 Citations (Scopus)

Abstract

Response surface methodology (RSM) was applied to optimize the oven drying conditions for lycopene content (Y1) and lipophilic antioxidant capacity (Y2) in decanter, a by-product of the pink guava puree industry. Two-factor central composite design was employed to determine the effects of two independent variables, namely temperature (X1: 50-80 °C) and drying time (X2: 4-6 h). Lycopene content and lipophilic antioxidant capacity were measured using high-performance liquid chromatography (HPLC) and the ABTS radicals scavenging assay, respectively. A β-carotene bleaching assay was also applied to measure the antioxidant activity. Response surface plots showed that an increase in temperature and time significantly reduced the response variables. The optimum oven conditions for drying of decanter with minimum lycopene degradation were 43.8 °C for 6.4 h, with a predicted lycopene content of 14 mg/100 g and antioxidant capacity of 21 μmol LE/100 g. To validate the optimized model, the experimental values were compared with the predicted values to check the adequacy of the model. The experimental values were found to be in agreement with those predicted, indicating the suitability of the model for optimizing the oven drying conditions for decanter.

Original languageEnglish
Pages (from-to)729-735
Number of pages7
JournalLWT - Food Science and Technology
Volume43
Issue number5
DOIs
Publication statusPublished - Jun 2010

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Psidium
guavas
lycopene
response surface methodology
ovens
Industry
Antioxidants
drying
industry
antioxidants
Temperature
assays
Carotenoids
carotenes
bleaching
temperature
Theoretical Models
high performance liquid chromatography
antioxidant activity
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Food Science

Cite this

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title = "Optimization of oven drying conditions for lycopene content and lipophilic antioxidant capacity in a by-product of the pink guava puree industry using response surface methodology",
abstract = "Response surface methodology (RSM) was applied to optimize the oven drying conditions for lycopene content (Y1) and lipophilic antioxidant capacity (Y2) in decanter, a by-product of the pink guava puree industry. Two-factor central composite design was employed to determine the effects of two independent variables, namely temperature (X1: 50-80 °C) and drying time (X2: 4-6 h). Lycopene content and lipophilic antioxidant capacity were measured using high-performance liquid chromatography (HPLC) and the ABTS radicals scavenging assay, respectively. A β-carotene bleaching assay was also applied to measure the antioxidant activity. Response surface plots showed that an increase in temperature and time significantly reduced the response variables. The optimum oven conditions for drying of decanter with minimum lycopene degradation were 43.8 °C for 6.4 h, with a predicted lycopene content of 14 mg/100 g and antioxidant capacity of 21 μmol LE/100 g. To validate the optimized model, the experimental values were compared with the predicted values to check the adequacy of the model. The experimental values were found to be in agreement with those predicted, indicating the suitability of the model for optimizing the oven drying conditions for decanter.",
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AU - Rajab, Nor Fadilah

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