Optimization of medium for the production of β-cyclodextrin glucanotransferase using Central Composite Design (CCD)

Hanan Moawia Ibrahim, Wan Mohtar Wan Yusoff, Aidil Abdul Hamid, Rosli Md Illias, Osman Hassan, Othman Omar

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Production of cyclodextrin glucanotransferase (CGTase) from Bacillus G1, a new bacterial isolate was optimized in shake-flask cultures using statistical design approach. An efficient fermentation medium producing CGTase with high activity (54.9 U/ml) was determined. A 25 Central Composite Design (CCD; half fraction) has been chosen to elucidate the combined effect of five process variables; tapioca starch, peptone, yeast extract, magnesium sulphate and potassium phosphate concentration. Concentrations of 4% tapioca starch, 2% peptone, 0.04% magnesium sulphate and 1% sodium carbonate have been shown to be optimum for the production of CGTase.

Original languageEnglish
Pages (from-to)753-758
Number of pages6
JournalProcess Biochemistry
Volume40
Issue number2
DOIs
Publication statusPublished - Feb 2005

Fingerprint

Cyclodextrins
Magnesium Sulfate
Manihot
Peptones
Starch
Magnesium
Composite materials
Batch Cell Culture Techniques
Bacilli
Yeast
Fermentation
Bacillus
Potassium
Carbonates
Phosphates
Yeasts
Sodium
cyclomaltodextrin glucanotransferase
Sulfates

Keywords

  • CCD
  • Cyclodextrin glucanotransferase
  • Medium optimization

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Engineering (miscellaneous)
  • Industrial and Manufacturing Engineering

Cite this

Optimization of medium for the production of β-cyclodextrin glucanotransferase using Central Composite Design (CCD). / Ibrahim, Hanan Moawia; Wan Yusoff, Wan Mohtar; Abdul Hamid, Aidil; Illias, Rosli Md; Hassan, Osman; Omar, Othman.

In: Process Biochemistry, Vol. 40, No. 2, 02.2005, p. 753-758.

Research output: Contribution to journalArticle

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