Optimization of FPase activity using sorghum straw planted in malaysia by aspergillus terreus SUK-1 via solid substrate fermentation

El Mubarak Musa Tibin Musa, Najeeb Kaid Nasser Al-Shorgani, Nawal Noureldaim Abuelhassan, Febri Doni, Wedad Hassan Abdelhaleem, Aidil Abdul Hamid, Mohd. Sahaid Kalil, Wan Mohtar Wan Yusoff

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The optimum conditions for cellulase activity measured as Filter paper activity (FPase) from sorghum straw by Aspergillus terreus SUK-1 using Response Surface Methodology (RSM) was based on Central Composite Design (CCD). The effect of incubation temperature, initial pH and moisture content on FPase activity was carried out in Solid Substrate Fermentation (SSF). The results were analysed by analysis of variance (ANOVA) and the regression quadratic model was obtained. ANOVA analysis indicated that the model was significant (p<0.05) and that the effect of temperature and moisture content significantly effected on the FPase activity. The optimized conditions are; temperature 25°C, pH 4 and moisture content of 40%, while the predicted FPase activity is 0.35 U mL-1. The model was validated by applying the optimized conditions and it was found that the FPase activity was 0.36 U mL-1 which indicate the validity of the model.

Original languageEnglish
Pages (from-to)23-28
Number of pages6
JournalBiotechnology
Volume14
Issue number1
DOIs
Publication statusPublished - 2015

Fingerprint

Sorghum
Aspergillus
Malaysia
Straw
Fermentation
Analysis of Variance
Temperature
Moisture
Substrates
Analysis of variance (ANOVA)
Cellulase
Thermodynamic properties
Composite materials

Keywords

  • Aspergillus terreus SUK-1
  • Filter paper activity
  • Response surface methodology
  • Sorghum straw

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Optimization of FPase activity using sorghum straw planted in malaysia by aspergillus terreus SUK-1 via solid substrate fermentation. / Musa, El Mubarak Musa Tibin; Al-Shorgani, Najeeb Kaid Nasser; Abuelhassan, Nawal Noureldaim; Doni, Febri; Abdelhaleem, Wedad Hassan; Abdul Hamid, Aidil; Kalil, Mohd. Sahaid; Wan Yusoff, Wan Mohtar.

In: Biotechnology, Vol. 14, No. 1, 2015, p. 23-28.

Research output: Contribution to journalArticle

Musa, El Mubarak Musa Tibin ; Al-Shorgani, Najeeb Kaid Nasser ; Abuelhassan, Nawal Noureldaim ; Doni, Febri ; Abdelhaleem, Wedad Hassan ; Abdul Hamid, Aidil ; Kalil, Mohd. Sahaid ; Wan Yusoff, Wan Mohtar. / Optimization of FPase activity using sorghum straw planted in malaysia by aspergillus terreus SUK-1 via solid substrate fermentation. In: Biotechnology. 2015 ; Vol. 14, No. 1. pp. 23-28.
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AU - Doni, Febri

AU - Abdelhaleem, Wedad Hassan

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AB - The optimum conditions for cellulase activity measured as Filter paper activity (FPase) from sorghum straw by Aspergillus terreus SUK-1 using Response Surface Methodology (RSM) was based on Central Composite Design (CCD). The effect of incubation temperature, initial pH and moisture content on FPase activity was carried out in Solid Substrate Fermentation (SSF). The results were analysed by analysis of variance (ANOVA) and the regression quadratic model was obtained. ANOVA analysis indicated that the model was significant (p<0.05) and that the effect of temperature and moisture content significantly effected on the FPase activity. The optimized conditions are; temperature 25°C, pH 4 and moisture content of 40%, while the predicted FPase activity is 0.35 U mL-1. The model was validated by applying the optimized conditions and it was found that the FPase activity was 0.36 U mL-1 which indicate the validity of the model.

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