Optimization of extraction parameters for antioxidant and total phenolic content of ficus carica L. Latex from white genoa cultivar

M. Shahinuzzaman, Zahira Yaakob, Norrakiah Abdullah Sani, Parul Akhtar, M. D. Zahidul Islam, M. S.T. Afsana Mimi, Shamsul Akmal Shamsudin

Research output: Contribution to journalArticle

Abstract

In this study, the extraction parameters for antioxidant activity and total phenolic content of Ficus carica L. latex from White Genoa cultivar were optimized using response surface methodology (RSM). Effect of temperature, extraction time and solvent concentration for maceration extraction were investigated. The antioxidant activity was determined with DPPH (1,1-diphenyl-2-picrylhydrazyl) assay and expressed as percent of inhibition. Total phenolic content (TPC) was determined with Folin-Ciocalteu assay and expressed as µg GAE/ mL. The optimum points that maximize the DPPH inhibition are 32 °C temperature, 31.50 min extraction time and 65 % ethanol. The optimum points for TPC are 32 °C temperature, 37.50 min extraction time and 70 % ethanol. The predicted DPPH inhibition and TPC at these predicted points are 67.08 % and 313.811 µg GAE/mL respectively. In this model, the F value (26.03 and 20.96), R-Squared value (0.9591 and 0.9497) and signal to noise ratio (13.431 and 12.799) are reasonable to fit the model.

Original languageEnglish
Pages (from-to)1859-1865
Number of pages7
JournalAsian Journal of Chemistry
Volume31
Issue number8
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

Parameter extraction
Latex
Antioxidants
Assays
Ethanol
Temperature
Signal to noise ratio
2,2-diphenyl-1-picrylhydrazyl

Keywords

  • Antioxidant
  • Ficus carica
  • Latex
  • Optimization
  • RSM
  • Total phenol

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Optimization of extraction parameters for antioxidant and total phenolic content of ficus carica L. Latex from white genoa cultivar. / Shahinuzzaman, M.; Yaakob, Zahira; Abdullah Sani, Norrakiah; Akhtar, Parul; Zahidul Islam, M. D.; Afsana Mimi, M. S.T.; Akmal Shamsudin, Shamsul.

In: Asian Journal of Chemistry, Vol. 31, No. 8, 01.01.2019, p. 1859-1865.

Research output: Contribution to journalArticle

Shahinuzzaman, M. ; Yaakob, Zahira ; Abdullah Sani, Norrakiah ; Akhtar, Parul ; Zahidul Islam, M. D. ; Afsana Mimi, M. S.T. ; Akmal Shamsudin, Shamsul. / Optimization of extraction parameters for antioxidant and total phenolic content of ficus carica L. Latex from white genoa cultivar. In: Asian Journal of Chemistry. 2019 ; Vol. 31, No. 8. pp. 1859-1865.
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