Optimization of extraction methods for determination of phosphodiesterase-5 (PDE5) inhibitors in premix coffee

A. M. Zailina, Aminah Abdullah, Mohd. Ambar Yarmo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An efficient analytical technique capable of analyzing three most common phosphodiesterase-5 (PDE5) inhibitors (vardenafil, sildenafil and tadalafil) simultaneously in premix coffee was developed. Sample extractions using either acetonitrile or methanol with two different extraction techniques (with and without evaporation steps) were evaluated. Identification and quantitation was conducted by high performance liquid chromatography with photo-diode-array (HPLC-DAD) at different wavelengths; 230 nm, 245 nm and 290 nm; and by time of flight mass spectrometry (LC-MS-TOF). Extraction with acetonitrile (without evaporation with nitrogen) showed recovery ranging from 105% to 113% (± <10%) for HPLC-DAD at 245 nm and 93% to 102% (± <2.5%) for LC-MS-TOF. Chromatogram separation was best achieved with mobile phase consisted of water (0.1% formic acid) and acetonitrile (0.1% formic acid) with gradient elution within 20 min. Thus, the results indicated that extraction using acetonitrile without evaporation step was the most efficient technique for determination of PDE5 inhibitors in premix coffee.

Original languageEnglish
Pages (from-to)135-142
Number of pages8
JournalSains Malaysiana
Volume42
Issue number2
Publication statusPublished - Feb 2013

Fingerprint

Phosphodiesterase 5 Inhibitors
Coffee
formic acid
Evaporation
High performance liquid chromatography
Mass spectrometry
Methanol
Diodes
Nitrogen
Recovery
Wavelength
acetonitrile
Water

Keywords

  • HPLC-DAD
  • LC-MS-TOF
  • Optimization
  • PDE5 inhibitors
  • Premix coffee

ASJC Scopus subject areas

  • General

Cite this

Optimization of extraction methods for determination of phosphodiesterase-5 (PDE5) inhibitors in premix coffee. / Zailina, A. M.; Abdullah, Aminah; Yarmo, Mohd. Ambar.

In: Sains Malaysiana, Vol. 42, No. 2, 02.2013, p. 135-142.

Research output: Contribution to journalArticle

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