Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (pangasius sutchi) skin

Fatemeh Mahmoodani, Venus Sanaei Ardekani, See Siau Fern, Salma Mohamad Yusop, Abd. Salam Babji

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In order to optimize the extraction of gelatin from pangasius catfish skin, a response surface method (RSM) involving a Central Composite Design (CCD) was applied. Four variables, namely NaOH concentration (0-0.3 N), acetic acid concentration (0.025-0.125 N), extraction time (2-4 h) and extraction temperature (40-80°C) were selected as independent variables for the optimization using RSM. The dependent variable was calculated by hydroxyproline recovery. The optimum conditions for extraction were produced by a pre-treatment of 0.2 N NaOH and 0.1 N acetic acid along with hot water extraction at 63.7°C for 2.41 h. The results showed that the predicted response by RSM (68.53%) closely matched the experimental response of 68.16%. The results indicated that the extracted gelatin possessed high gel strength (438 g) and high content of imino acid (proline and hydroxyproline) (18.01%) with a viscosity of 4.67 mPas. The results showed that RSM was a great optimizing tool for extraction of gelatin from pangasius catfish skin. The gelatin was also proven to have significantly (p<0.05) higher quality of physicochemical properties than those from bovine skin gelatin.

Original languageEnglish
Pages (from-to)995-1002
Number of pages8
JournalSains Malaysiana
Volume43
Issue number7
Publication statusPublished - 2014

Fingerprint

Pangasius
Pangasianodon hypophthalmus
gelatin
skin (animal)
catfish
physicochemical properties
hydroxyproline
acetic acid
gel strength
methodology
proline
viscosity
pretreatment
cattle

Keywords

  • Gelatin
  • Optimization
  • Pangasius catfish
  • Physicochemical properties
  • Response surface method

ASJC Scopus subject areas

  • General

Cite this

Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (pangasius sutchi) skin. / Mahmoodani, Fatemeh; Ardekani, Venus Sanaei; Fern, See Siau; Mohamad Yusop, Salma; Babji, Abd. Salam.

In: Sains Malaysiana, Vol. 43, No. 7, 2014, p. 995-1002.

Research output: Contribution to journalArticle

Mahmoodani, Fatemeh ; Ardekani, Venus Sanaei ; Fern, See Siau ; Mohamad Yusop, Salma ; Babji, Abd. Salam. / Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (pangasius sutchi) skin. In: Sains Malaysiana. 2014 ; Vol. 43, No. 7. pp. 995-1002.
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