Optimization of enzymatic hydrolysis of tilapia (Oreochromis Spp.) scale gelatine

Abdul Wahab Mohammad, Abd Ghani Kumar, Roseliza Kadir Basha

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Gelatine hydrolysate is a type of gelatine that undergoes a controlled hydrolysis treatment to further break apart the gelatine or collagen molecules. In this study, gelatine hydrolysate was produced from commercial tilapia scale gelatine via controlled enzymatic hydrolysis. Commercial Alcalase 2.4 L, a protease enzyme was used to breakdown the peptide chains present in the gelatine. Optimization of hydrolysis conditions (temperature, time and enzyme to substrate ratio) was conducted by utilizing response surface methodology (RSM). Results showed that a hydrolysis temperature of 57.6 °C together with a hydrolysis time of 80 min and enzyme to substrate ratio of 1.20 % (v/w) were the optimum conditions to obtain the highest degree of hydrolysis (10.91 %). The freeze-dried gelatine hydrolysate was characterized with respect to chemical composition:approximate composition, viscosity and molecular weight. The gelatine hydrolysate produced contained a high content of protein (85.26 %); thus, it may serve as a potential protein source for human needs.

Original languageEnglish
Pages (from-to)27-39
Number of pages13
JournalInternational Aquatic Research
Volume7
Issue number1
DOIs
Publication statusPublished - 2014

Fingerprint

Tilapia (Cichlidae)
Oreochromis
enzymatic hydrolysis
gelatin
hydrolysis
hydrolysates
enzyme
substrate
protein
enzymes
collagen
peptide
subtilisin
viscosity
temperature
response surface methodology
chemical composition
protein sources
proteinases
protein content

Keywords

  • Enzymatic hydrolysis
  • Gelatine hydrolysate
  • Optimization
  • RSM

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Optimization of enzymatic hydrolysis of tilapia (Oreochromis Spp.) scale gelatine. / Mohammad, Abdul Wahab; Kumar, Abd Ghani; Basha, Roseliza Kadir.

In: International Aquatic Research, Vol. 7, No. 1, 2014, p. 27-39.

Research output: Contribution to journalArticle

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