Optimization of enzymatic hydrolysis of palm kernel cake protein (PKCP) for producing hydrolysates with antiradical capacity

Khar Ling Ng, Mohd. Khan Ayob, Mamot Said, Md Anuar Osman, Amin Ismail

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The enzymatic hydrolysis of palm kernel cake protein (PKCP) with trypsin to obtain PKCP hydrolysates (PKCPH) was optimized using response surface methodology (RSM). A central composite design (CCD) was used to study the influence of four independent variables, namely pH, hydrolysis temperature (°C), substrate concentration (w/v) and enzyme/substrate (w/w) ratio on the degree of hydrolysis (DH%). The hydrolysis was carried out using different combinations of four hydrolytic parameters at five levels for 6h. The CCD consisted of 24 experimental points and six replicates of the central points. The data were analyzed using Design-Expert Software. The results showed that all of the variables evaluated significantly influenced the DH% in a second polynomial model, and different combinations of parameters were generated to obtain three different levels of DH (30%, 40% and 50%), namely PKCPH 30, PKCPH 40 and PKCPH 50. The PKCPH with different DH% showed significantly different antiradical properties (p<0.05). The PKCPH 50 preparation had the lowest EC 50 value for DPPH radical scavenging capacity (0.14mg/ml). In the ABTS + radical scavenging capacity and PCL-ACW (photo chemiluminescence-antiradical capacity of water soluble substances) assays, PKCPH 50 showed the highest Trolox equivalent antioxidant capacity value (326.67±5.77μmol TEAC/g) and ascorbic acid equivalent value (11.43±0.03μg AAE/mg) of the preparations tested. Moreover, the protein hydrolysates also exhibited a notable reducing effect in a dose-dependent manner. Optimum conditions for enzymatic hydrolysis of PKCP were established in this study to produce an antiradical agent.

Original languageEnglish
Pages (from-to)725-731
Number of pages7
JournalIndustrial Crops and Products
Volume43
Issue number1
DOIs
Publication statusPublished - May 2013

Fingerprint

palm kernel cake
enzymatic hydrolysis
hydrolysates
hydrolysis
proteins
protein hydrolysates
enzyme substrates
chemiluminescence
response surface methodology
trypsin
ascorbic acid
antioxidants
assays
dosage

Keywords

  • Antioxidant
  • Antiradical
  • Hydrolysate
  • Optimization
  • PKCP (palm kernel cake protein)
  • Trypsin

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Optimization of enzymatic hydrolysis of palm kernel cake protein (PKCP) for producing hydrolysates with antiradical capacity. / Ng, Khar Ling; Ayob, Mohd. Khan; Said, Mamot; Osman, Md Anuar; Ismail, Amin.

In: Industrial Crops and Products, Vol. 43, No. 1, 05.2013, p. 725-731.

Research output: Contribution to journalArticle

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