Optimization of CMCase production from sorghum straw by Aspergillus terreus SUK-1 under solid substrate fermentation using response surface methodology

El Mubarak Musa Tibin, Najeeb Kaid Naseer Al-Shorgani, Nawal Noureldaim Abuelhassan, Aidil Abdul Hamid, Mohd. Sahaid Kalil, Wan Mohtar Wan Yusoff

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The cellulase production using sorghum straw as substrate by fungal culture of Aspergillus terreus SUK-1 was investigated in solid substrate fermentation (SSF). The optimum CMCase was achieved by testing most effective fermentation parameters which were: incubation temperature, pH and moisture content using Response Surface Methodology (RSM) based on Central Composite Design (CCD).The carboxymethyl cellulase activity (CMCase) was measured as the defining factor. The results were analysed by analysis of variance (ANOVA) and the regression quadratic model was obtained. The model was found to be significant (p<0.05) and the effect of temperature (25-40°C) and pH (4-7) was found to be not significant on CMCase activity whereas the moisture content was significant in the SSF conditions employed. The high yield of predicted CMCase activity (0.2 U/ml) was obtained under the optimized conditions (temperature 40 °C, pH 5.4 and moisture content of 80%). The model was validated by applying the optimized conditions and it was found that the model was valid.

Original languageEnglish
Title of host publicationAIP Conference Proceedings
Pages184-190
Number of pages7
Volume1571
DOIs
Publication statusPublished - 2013
Event2013 UKM Faculty of Science and Technology Post-Graduate Colloquium - Selangor
Duration: 3 Jul 20134 Jul 2013

Other

Other2013 UKM Faculty of Science and Technology Post-Graduate Colloquium
CitySelangor
Period3/7/134/7/13

Fingerprint

sorghum
Aspergillus
fermentation
methodology
optimization
moisture content
analysis of variance
temperature
regression analysis
composite materials

Keywords

  • Aspergillus terreus SUK-1
  • Carboxymethylcellulase activity
  • Optimization
  • Response Surface methodology
  • Sorghum straw

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Tibin, E. M. M., Al-Shorgani, N. K. N., Abuelhassan, N. N., Abdul Hamid, A., Kalil, M. S., & Wan Yusoff, W. M. (2013). Optimization of CMCase production from sorghum straw by Aspergillus terreus SUK-1 under solid substrate fermentation using response surface methodology. In AIP Conference Proceedings (Vol. 1571, pp. 184-190) https://doi.org/10.1063/1.4858652

Optimization of CMCase production from sorghum straw by Aspergillus terreus SUK-1 under solid substrate fermentation using response surface methodology. / Tibin, El Mubarak Musa; Al-Shorgani, Najeeb Kaid Naseer; Abuelhassan, Nawal Noureldaim; Abdul Hamid, Aidil; Kalil, Mohd. Sahaid; Wan Yusoff, Wan Mohtar.

AIP Conference Proceedings. Vol. 1571 2013. p. 184-190.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tibin, EMM, Al-Shorgani, NKN, Abuelhassan, NN, Abdul Hamid, A, Kalil, MS & Wan Yusoff, WM 2013, Optimization of CMCase production from sorghum straw by Aspergillus terreus SUK-1 under solid substrate fermentation using response surface methodology. in AIP Conference Proceedings. vol. 1571, pp. 184-190, 2013 UKM Faculty of Science and Technology Post-Graduate Colloquium, Selangor, 3/7/13. https://doi.org/10.1063/1.4858652
Tibin, El Mubarak Musa ; Al-Shorgani, Najeeb Kaid Naseer ; Abuelhassan, Nawal Noureldaim ; Abdul Hamid, Aidil ; Kalil, Mohd. Sahaid ; Wan Yusoff, Wan Mohtar. / Optimization of CMCase production from sorghum straw by Aspergillus terreus SUK-1 under solid substrate fermentation using response surface methodology. AIP Conference Proceedings. Vol. 1571 2013. pp. 184-190
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