Optimization of biometabolite production by streptomyces SP PT1 in submerged culture for improved in vitro antibacterial and anti-oxidant activity

Zidan Abduldiem Bashir, Gires Usup, Asmat Ahmad, Shukor Md. Nor

Research output: Contribution to journalArticle

Abstract

A 30-run Box-Behnken design (BBD) was applied to statistically optimize the production of biometabolite from Streptomyces sp PT1 in submerged culture by varying four factors: nitrogen (yeast extract) concentration, MnCl2 FeSO4 and Nad. Two antimicrobial assays (using Bacillus subtilis and Vibrio parahaemoliticus as test organisms) and three anti-oxidant assays were incorporated to test for the activity of the produced biometabolite. For antimicrobial assay conducted using Bacillus subtilis (BS) as test organism, the optimum extraction conditions consisted of a basic medium composed of: nitrogen (10 g/L), FeSO47H2O (0.35 g/100mL), MnCl2 4H2O (0.3 g/100mL) and NaCl (4.0 g/L). For CUPRAC and DPPH antioxidant assays, optimum results were found with the use of a basic medium comprising of: nitrogen (20 g/L), FeSO4 7H2O (1.0 g/100mL), MnCl2 4H2O (0.3 g/100mL) and NaCl (2.0 g/L). For FRAP antioxidant assay, optimum results were found with the use of a basic medium comprising of: nitrogen (2 g/L), FeSO4 7H2O (0.135 g/100mL), MnCl2 4H20 (0.3 g/100mL) and NaCl (4.0 g/L). For the metabolite produced by Streptomyces sp PT1 in submerged culture, a combination of Plackett-Burman design and Box-Behnken Response Surface Methodology was thus found effective to optimize medium components producing up to 66.11%, 34.29%, 12.46%, 28.44% and 27.53% increase in responses for antimicrobial (B. subtilis, Vibro parahaemoliticus) and antioxidant assays (FRAP, CUPRAC and DPPH) respectively.

Original languageEnglish
Pages (from-to)497-507
Number of pages11
JournalAsian Journal of Microbiology, Biotechnology and Environmental Sciences
Volume16
Issue number3
Publication statusPublished - 2014

Fingerprint

Streptomyces
Oxidants
antioxidant
assay
Nitrogen
Bacillus subtilis
Antioxidants
nitrogen
Vibrio
Yeasts
yeast
In Vitro Techniques
manganese chloride
metabolite

Keywords

  • Anti-oxidant activity
  • Biometabolite production
  • Streptomyces

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Environmental Science(all)

Cite this

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title = "Optimization of biometabolite production by streptomyces SP PT1 in submerged culture for improved in vitro antibacterial and anti-oxidant activity",
abstract = "A 30-run Box-Behnken design (BBD) was applied to statistically optimize the production of biometabolite from Streptomyces sp PT1 in submerged culture by varying four factors: nitrogen (yeast extract) concentration, MnCl2 FeSO4 and Nad. Two antimicrobial assays (using Bacillus subtilis and Vibrio parahaemoliticus as test organisms) and three anti-oxidant assays were incorporated to test for the activity of the produced biometabolite. For antimicrobial assay conducted using Bacillus subtilis (BS) as test organism, the optimum extraction conditions consisted of a basic medium composed of: nitrogen (10 g/L), FeSO47H2O (0.35 g/100mL), MnCl2 4H2O (0.3 g/100mL) and NaCl (4.0 g/L). For CUPRAC and DPPH antioxidant assays, optimum results were found with the use of a basic medium comprising of: nitrogen (20 g/L), FeSO4 7H2O (1.0 g/100mL), MnCl2 4H2O (0.3 g/100mL) and NaCl (2.0 g/L). For FRAP antioxidant assay, optimum results were found with the use of a basic medium comprising of: nitrogen (2 g/L), FeSO4 7H2O (0.135 g/100mL), MnCl2 4H20 (0.3 g/100mL) and NaCl (4.0 g/L). For the metabolite produced by Streptomyces sp PT1 in submerged culture, a combination of Plackett-Burman design and Box-Behnken Response Surface Methodology was thus found effective to optimize medium components producing up to 66.11{\%}, 34.29{\%}, 12.46{\%}, 28.44{\%} and 27.53{\%} increase in responses for antimicrobial (B. subtilis, Vibro parahaemoliticus) and antioxidant assays (FRAP, CUPRAC and DPPH) respectively.",
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T1 - Optimization of biometabolite production by streptomyces SP PT1 in submerged culture for improved in vitro antibacterial and anti-oxidant activity

AU - Bashir, Zidan Abduldiem

AU - Usup, Gires

AU - Ahmad, Asmat

AU - Md. Nor, Shukor

PY - 2014

Y1 - 2014

N2 - A 30-run Box-Behnken design (BBD) was applied to statistically optimize the production of biometabolite from Streptomyces sp PT1 in submerged culture by varying four factors: nitrogen (yeast extract) concentration, MnCl2 FeSO4 and Nad. Two antimicrobial assays (using Bacillus subtilis and Vibrio parahaemoliticus as test organisms) and three anti-oxidant assays were incorporated to test for the activity of the produced biometabolite. For antimicrobial assay conducted using Bacillus subtilis (BS) as test organism, the optimum extraction conditions consisted of a basic medium composed of: nitrogen (10 g/L), FeSO47H2O (0.35 g/100mL), MnCl2 4H2O (0.3 g/100mL) and NaCl (4.0 g/L). For CUPRAC and DPPH antioxidant assays, optimum results were found with the use of a basic medium comprising of: nitrogen (20 g/L), FeSO4 7H2O (1.0 g/100mL), MnCl2 4H2O (0.3 g/100mL) and NaCl (2.0 g/L). For FRAP antioxidant assay, optimum results were found with the use of a basic medium comprising of: nitrogen (2 g/L), FeSO4 7H2O (0.135 g/100mL), MnCl2 4H20 (0.3 g/100mL) and NaCl (4.0 g/L). For the metabolite produced by Streptomyces sp PT1 in submerged culture, a combination of Plackett-Burman design and Box-Behnken Response Surface Methodology was thus found effective to optimize medium components producing up to 66.11%, 34.29%, 12.46%, 28.44% and 27.53% increase in responses for antimicrobial (B. subtilis, Vibro parahaemoliticus) and antioxidant assays (FRAP, CUPRAC and DPPH) respectively.

AB - A 30-run Box-Behnken design (BBD) was applied to statistically optimize the production of biometabolite from Streptomyces sp PT1 in submerged culture by varying four factors: nitrogen (yeast extract) concentration, MnCl2 FeSO4 and Nad. Two antimicrobial assays (using Bacillus subtilis and Vibrio parahaemoliticus as test organisms) and three anti-oxidant assays were incorporated to test for the activity of the produced biometabolite. For antimicrobial assay conducted using Bacillus subtilis (BS) as test organism, the optimum extraction conditions consisted of a basic medium composed of: nitrogen (10 g/L), FeSO47H2O (0.35 g/100mL), MnCl2 4H2O (0.3 g/100mL) and NaCl (4.0 g/L). For CUPRAC and DPPH antioxidant assays, optimum results were found with the use of a basic medium comprising of: nitrogen (20 g/L), FeSO4 7H2O (1.0 g/100mL), MnCl2 4H2O (0.3 g/100mL) and NaCl (2.0 g/L). For FRAP antioxidant assay, optimum results were found with the use of a basic medium comprising of: nitrogen (2 g/L), FeSO4 7H2O (0.135 g/100mL), MnCl2 4H20 (0.3 g/100mL) and NaCl (4.0 g/L). For the metabolite produced by Streptomyces sp PT1 in submerged culture, a combination of Plackett-Burman design and Box-Behnken Response Surface Methodology was thus found effective to optimize medium components producing up to 66.11%, 34.29%, 12.46%, 28.44% and 27.53% increase in responses for antimicrobial (B. subtilis, Vibro parahaemoliticus) and antioxidant assays (FRAP, CUPRAC and DPPH) respectively.

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