Optimization and maximization of hexavalent molybdenum reduction to Mo-blue by Serratia sp. strain MIE2 using response surface methodology

Mohd Izuan Effendi Bin Halmi, Siti Rozaimah Sheikh Abdullah, Helmi Wasoh, Wan Lutfi Wan Johari, Mohd Shukuri Bin Mohd Ali, Noor Azmi Shaharuddin, Mohd Yunus Shukor

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Molybdenum has long been known to be toxic to ruminants, but not to humans. However, more recently it has been increasingly reported that molybdenum shows toxic effects to reproductive organs of fish, mouse and even humans. Hence, its removal from the environment is highly sought after. In this study, response surface methodology (RSM) was successfully applied in the optimization and maximization of Mo6+ reduction to Mo-blue by Serratia sp. MIE2 for future bioremediation application. The optimal conditions predicted by RSM were 20 mM molybdate, 3.95 mM phosphate, pH 6.25 and 25 g l−1 sucrose with absorbance of 19.53 for Mo-blue production measured at 865 nm. The validation experimental run of the predicted optimal conditions showed that the maximum Mo-blue production occurred at absorbance of 20.87, with a 6.75 % deviation from the predicted value obtained from RSM. Molybdate reduction was successfully maximized using RSM with molybdate reduction before and after optimization using RSM showing Mo-blue production starting at the absorbance value of 10.0 at 865 nm going up to an absorbance value above 20.87. The modelling kinetics of Mo6+ reduction showed that Teissier was the best model, with calculated Pmax, Ks and Ki values of 1.97 Mo-blue per hour, 5.79 mM and 31.48 mM, respectively.

Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalATTI Della Accademia Nazionale Dei Lincei Rendiconti Lincei Scienze Fisiche E Naturali
DOIs
Publication statusAccepted/In press - 8 Jul 2016

Fingerprint

Serratia
Molybdenum
molybdenum
response surface methodology
absorbance
molybdates
Poisons
Environmental Biodegradation
Ruminants
Sucrose
Fishes
Phosphates
ruminant
bioremediation
sucrose
gonads
ruminants
phosphate
molybdate
phosphates

Keywords

  • Bioremediation
  • Hexavalent molybdenum
  • Modelling kinetics
  • Response surface methodology

ASJC Scopus subject areas

  • Earth and Planetary Sciences(all)
  • Agricultural and Biological Sciences(all)
  • Environmental Science(all)

Cite this

Optimization and maximization of hexavalent molybdenum reduction to Mo-blue by Serratia sp. strain MIE2 using response surface methodology. / Halmi, Mohd Izuan Effendi Bin; Sheikh Abdullah, Siti Rozaimah; Wasoh, Helmi; Johari, Wan Lutfi Wan; Ali, Mohd Shukuri Bin Mohd; Shaharuddin, Noor Azmi; Shukor, Mohd Yunus.

In: ATTI Della Accademia Nazionale Dei Lincei Rendiconti Lincei Scienze Fisiche E Naturali, 08.07.2016, p. 1-13.

Research output: Contribution to journalArticle

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AU - Shaharuddin, Noor Azmi

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