Optimisation of urea complexation by box-behnken design

Chin Yoong Fei, Jumat Salimon, Mamot Said

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Urea complexation is a method favoured by researchers to separate fatty acids based on molecular structure. In this study, effects of urea amount, crystallisation temperature and crystallisation time on the final products of urea complexation were examined. Box-Behnken Design (BBD) was employed to study the significance of these factors and the optimum conditions for the technique were predicted and verified. Results showed that urea-to-polyunsaturated fatty acid (PUFA) mole ratio and crystallisation temperature were two significant variables for enrichment of PUFA in non-urea complexing fraction (NUCF). In a 17-point experimental design, percentage of saturated fatty acid (SFA) was reduced almost entirely from initial 4.49%, monounsaturated fatty acid (MUFA) reduced from 57.02% to 4132%, while PUFA increased from 33.49% to 53.87% in NUCF. Optimum condition for maximum PUFA and minimum SFA and MUFA contents was predicted at urea-to-PUFA mole ratio of 20, crystallisation temperature of -20°C and crystallisation time of 29.67 h.

Original languageEnglish
Pages (from-to)795-803
Number of pages9
JournalSains Malaysiana
Volume39
Issue number5
Publication statusPublished - Oct 2010

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crystallization
polyunsaturated fatty acids
urea
monounsaturated fatty acids
saturated fatty acids
temperature
chemical structure
researchers
experimental design
fatty acid composition
fatty acids
methodology

Keywords

  • Box-Behnken design (BBD)
  • Optimisation
  • Polyunsaturated fatty acid (PUFA)
  • Urea complexation

ASJC Scopus subject areas

  • General

Cite this

Fei, C. Y., Salimon, J., & Said, M. (2010). Optimisation of urea complexation by box-behnken design. Sains Malaysiana, 39(5), 795-803.

Optimisation of urea complexation by box-behnken design. / Fei, Chin Yoong; Salimon, Jumat; Said, Mamot.

In: Sains Malaysiana, Vol. 39, No. 5, 10.2010, p. 795-803.

Research output: Contribution to journalArticle

Fei, CY, Salimon, J & Said, M 2010, 'Optimisation of urea complexation by box-behnken design', Sains Malaysiana, vol. 39, no. 5, pp. 795-803.
Fei CY, Salimon J, Said M. Optimisation of urea complexation by box-behnken design. Sains Malaysiana. 2010 Oct;39(5):795-803.
Fei, Chin Yoong ; Salimon, Jumat ; Said, Mamot. / Optimisation of urea complexation by box-behnken design. In: Sains Malaysiana. 2010 ; Vol. 39, No. 5. pp. 795-803.
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