Optimisation of LS54/dxaqueous two phase systemconditions for cutinase recovery

Jamaliah Md Jahim, Abdul Wahab Mohammad, Fariza Akmal Abdul Mutalib, Farah Diba Abu Bakar, Osman Hassan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Aqueous two phase system comprising Dehypon® LS 54 and K4484 Dextrin® was selected for recovery of cutinase enzyme. Parameters such as pH, system composition and type of salt as an additive, influenced the protein partitioning behaviour and optimisation of these parameters become necessary to be done in the design of primary recovery process of ATPS. The cutinase partitioning experiments were carried out with 30% of cutinase solution added to LS 54/Dx system. Results showed that cutinase enzyme preferred to partition into LS 54 rich-phase at pH 8.0 and the affinity of cutinase into top phase was observed higher with the increment of system compositions, which represented by tie line length (TLL). Furthermore,the addition of 50mM salts such as K2SO4and KCl into LS 54/Dx system has led to raise partition coefficient of cutinase, kcutto 2.2 and 1.95 fold, respectively. The dependence of kcuton various additives such as (NH4)2SO4, Na2SO4and K2SO4at the same concentration, suggested that the addition of selected ions could enhance positive electrostatic potential which could attract more cutinase to partition into LS54 rich phase. As conclusion, the best conditions obtained for cutinase partitioning were pH8.0, TLL = 23% and Na2SO4= 50mM, from which the maximum kcut of 2.83 with improved recovery of cutinase in top phase up to 79% can be achieved.

Original languageEnglish
Pages (from-to)61-69
Number of pages9
JournalASEAN Journal of Chemical Engineering
Volume12
Issue number2
Publication statusPublished - 2012

Fingerprint

Recovery
Enzymes
Salts
Chemical analysis
Electrostatics
Proteins
Ions
cutinase
Experiments

Keywords

  • Aqueous two phase
  • Enzyme recovery
  • Optimise
  • Polymer-polymer system
  • Salt additives
  • System parameters

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

Optimisation of LS54/dxaqueous two phase systemconditions for cutinase recovery. / Md Jahim, Jamaliah; Mohammad, Abdul Wahab; Mutalib, Fariza Akmal Abdul; Abu Bakar, Farah Diba; Hassan, Osman.

In: ASEAN Journal of Chemical Engineering, Vol. 12, No. 2, 2012, p. 61-69.

Research output: Contribution to journalArticle

@article{245ca2964777437d892fbccae3c59f4f,
title = "Optimisation of LS54/dxaqueous two phase systemconditions for cutinase recovery",
abstract = "Aqueous two phase system comprising Dehypon{\circledR} LS 54 and K4484 Dextrin{\circledR} was selected for recovery of cutinase enzyme. Parameters such as pH, system composition and type of salt as an additive, influenced the protein partitioning behaviour and optimisation of these parameters become necessary to be done in the design of primary recovery process of ATPS. The cutinase partitioning experiments were carried out with 30{\%} of cutinase solution added to LS 54/Dx system. Results showed that cutinase enzyme preferred to partition into LS 54 rich-phase at pH 8.0 and the affinity of cutinase into top phase was observed higher with the increment of system compositions, which represented by tie line length (TLL). Furthermore,the addition of 50mM salts such as K2SO4and KCl into LS 54/Dx system has led to raise partition coefficient of cutinase, kcutto 2.2 and 1.95 fold, respectively. The dependence of kcuton various additives such as (NH4)2SO4, Na2SO4and K2SO4at the same concentration, suggested that the addition of selected ions could enhance positive electrostatic potential which could attract more cutinase to partition into LS54 rich phase. As conclusion, the best conditions obtained for cutinase partitioning were pH8.0, TLL = 23{\%} and Na2SO4= 50mM, from which the maximum kcut of 2.83 with improved recovery of cutinase in top phase up to 79{\%} can be achieved.",
keywords = "Aqueous two phase, Enzyme recovery, Optimise, Polymer-polymer system, Salt additives, System parameters",
author = "{Md Jahim}, Jamaliah and Mohammad, {Abdul Wahab} and Mutalib, {Fariza Akmal Abdul} and {Abu Bakar}, {Farah Diba} and Osman Hassan",
year = "2012",
language = "English",
volume = "12",
pages = "61--69",
journal = "ASEAN Journal of Chemical Engineering",
issn = "1655-4418",
publisher = "ASEAN Journal Ad Hoc Commitee, De La Salle University-Manilla",
number = "2",

}

TY - JOUR

T1 - Optimisation of LS54/dxaqueous two phase systemconditions for cutinase recovery

AU - Md Jahim, Jamaliah

AU - Mohammad, Abdul Wahab

AU - Mutalib, Fariza Akmal Abdul

AU - Abu Bakar, Farah Diba

AU - Hassan, Osman

PY - 2012

Y1 - 2012

N2 - Aqueous two phase system comprising Dehypon® LS 54 and K4484 Dextrin® was selected for recovery of cutinase enzyme. Parameters such as pH, system composition and type of salt as an additive, influenced the protein partitioning behaviour and optimisation of these parameters become necessary to be done in the design of primary recovery process of ATPS. The cutinase partitioning experiments were carried out with 30% of cutinase solution added to LS 54/Dx system. Results showed that cutinase enzyme preferred to partition into LS 54 rich-phase at pH 8.0 and the affinity of cutinase into top phase was observed higher with the increment of system compositions, which represented by tie line length (TLL). Furthermore,the addition of 50mM salts such as K2SO4and KCl into LS 54/Dx system has led to raise partition coefficient of cutinase, kcutto 2.2 and 1.95 fold, respectively. The dependence of kcuton various additives such as (NH4)2SO4, Na2SO4and K2SO4at the same concentration, suggested that the addition of selected ions could enhance positive electrostatic potential which could attract more cutinase to partition into LS54 rich phase. As conclusion, the best conditions obtained for cutinase partitioning were pH8.0, TLL = 23% and Na2SO4= 50mM, from which the maximum kcut of 2.83 with improved recovery of cutinase in top phase up to 79% can be achieved.

AB - Aqueous two phase system comprising Dehypon® LS 54 and K4484 Dextrin® was selected for recovery of cutinase enzyme. Parameters such as pH, system composition and type of salt as an additive, influenced the protein partitioning behaviour and optimisation of these parameters become necessary to be done in the design of primary recovery process of ATPS. The cutinase partitioning experiments were carried out with 30% of cutinase solution added to LS 54/Dx system. Results showed that cutinase enzyme preferred to partition into LS 54 rich-phase at pH 8.0 and the affinity of cutinase into top phase was observed higher with the increment of system compositions, which represented by tie line length (TLL). Furthermore,the addition of 50mM salts such as K2SO4and KCl into LS 54/Dx system has led to raise partition coefficient of cutinase, kcutto 2.2 and 1.95 fold, respectively. The dependence of kcuton various additives such as (NH4)2SO4, Na2SO4and K2SO4at the same concentration, suggested that the addition of selected ions could enhance positive electrostatic potential which could attract more cutinase to partition into LS54 rich phase. As conclusion, the best conditions obtained for cutinase partitioning were pH8.0, TLL = 23% and Na2SO4= 50mM, from which the maximum kcut of 2.83 with improved recovery of cutinase in top phase up to 79% can be achieved.

KW - Aqueous two phase

KW - Enzyme recovery

KW - Optimise

KW - Polymer-polymer system

KW - Salt additives

KW - System parameters

UR - http://www.scopus.com/inward/record.url?scp=84885139432&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885139432&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84885139432

VL - 12

SP - 61

EP - 69

JO - ASEAN Journal of Chemical Engineering

JF - ASEAN Journal of Chemical Engineering

SN - 1655-4418

IS - 2

ER -