Optimal physical and nutrient parameters for growth of Trichoderma virens UKMP-1M for heavy crude oil degradation

Ainon Hamzah, Mazni Abu Zarin, Aidil Abdul Hamid, Othman Omar, Sahidan Senafi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

This study was carried out to determine the optimal parameters for the production of biomass of Trichoderma virens UKMP-1M, a fungus isolated from oil-polluted wastewater. The isolate showed maximum growth at day six after incubation in Mineral Salt Medium (MSM) in the presence of 3% (v/v) heavy Khefji Sour crude oil. Although it grew at pH between 5.0 and 7.0, it grew best at pH 5.5. T. virens UKMP-1Mgrew at temperatures between 25°C and 35°C, with its highest growth at 30°C. Aeration by agitation at 200 rpm was shown to yield the greatest biomass. Peptone at concentration of 1.5% (w/v) was determined to be a better nitrogen source than urea, potassium nitrate (KNO 3), yeast extract, ammonium sulphate ((NH 4) 2SO 4) and ammonium chloride (NH 4Cl). Addition of 1% (v/v) crude oil to the MSM medium led to higher biomass production than the addition of 3%, 5%, 7% and 10% (v/v) crude oil. The result also revealed that 40% of total petroleum hydrocarbon (TPH), 100% of pristane and 74% of phytane compounds were degraded after 9 days of incubation at optimal physical and nutrient parameters.

Original languageEnglish
Pages (from-to)71-79
Number of pages9
JournalSains Malaysiana
Volume41
Issue number1
Publication statusPublished - Jan 2012

Fingerprint

Trichoderma virens
oils
degradation
nutrients
biomass production
minerals
salts
potassium nitrate
ammonium chloride
agitation
peptones
yeast extract
aeration
ammonium sulfate
petroleum
wastewater
hydrocarbons
urea
fungi
biomass

Keywords

  • Crude oil
  • Optimisation
  • Total petroleum hydrocarbon
  • Trichoderma virens

ASJC Scopus subject areas

  • General

Cite this

Optimal physical and nutrient parameters for growth of Trichoderma virens UKMP-1M for heavy crude oil degradation. / Hamzah, Ainon; Zarin, Mazni Abu; Abdul Hamid, Aidil; Omar, Othman; Senafi, Sahidan.

In: Sains Malaysiana, Vol. 41, No. 1, 01.2012, p. 71-79.

Research output: Contribution to journalArticle

Hamzah, Ainon ; Zarin, Mazni Abu ; Abdul Hamid, Aidil ; Omar, Othman ; Senafi, Sahidan. / Optimal physical and nutrient parameters for growth of Trichoderma virens UKMP-1M for heavy crude oil degradation. In: Sains Malaysiana. 2012 ; Vol. 41, No. 1. pp. 71-79.
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