Abstract
Optical biosensor based on hemoglobin (Hb) immobilized on the poly(n-butyl acrylate-co-N-acryloxysuccinimide) microspheres has been developed for the detection of nitrite (NO2 -) ion concentration. The iron HEM complex in the Hb catalyzed the reduction of NO2 - ion to nitric oxide (NO) followed by binding with deoxyhemoglobin (HbFe2+) to form the yellowish green iron-nitrosyl-hemoglobin (HbFe2+-NO) complex. Fiber optic reflectance spectrophotometer was used to monitor the NO2 - ion concentration quantitatively based on colour change of the immobilized Hb on the polyacrylate microspheres from reddish brown to yellowish green at the maximum reflectance wavelength of 668 nm. Characterization of the reflectometric nitrite biosensor encompassed pH effect, Hb concentration effect, linear response range, reproducibility, shelf life and ion interference effect studies had been conducted. The developed optical NO2 - ion biosensor exhibited dynamic linear range from 5 to 50 mg mL-1 (R2=0.9894) at pH7.0 with a limit of detection (LOD) of 3.3 mg mL-1 and reproducibility relative standard deviation (RSD) value of the biosensor at 5.8%. The shelf life of the optical nitrite biosensor is about 36 day and majority foreign ions that are often co-exist with NO2 - ion in the natural water sample did not show significant interference effect towards the determination of NO2 - ion using the developed optical biosensor except Hg2+, Ag+, Br- and S2- ions.
| Original language | Malay |
|---|---|
| Pages (from-to) | 2027-2033 |
| Number of pages | 7 |
| Journal | Sains Malaysiana |
| Volume | 47 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 1 Jan 2018 |
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Biosensor nitrit optik berasaskan hemoglobin terpegun pada mikrosfera poliakrilat. / Haslan, Syamimi; Tan @ Chong, Ling Ling; Lee, Yook Heng; Goh, Choo Ta.
In: Sains Malaysiana, Vol. 47, No. 9, 01.01.2018, p. 2027-2033.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biosensor nitrit optik berasaskan hemoglobin terpegun pada mikrosfera poliakrilat
AU - Haslan, Syamimi
AU - Tan @ Chong, Ling Ling
AU - Lee, Yook Heng
AU - Goh, Choo Ta
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Optical biosensor based on hemoglobin (Hb) immobilized on the poly(n-butyl acrylate-co-N-acryloxysuccinimide) microspheres has been developed for the detection of nitrite (NO2 -) ion concentration. The iron HEM complex in the Hb catalyzed the reduction of NO2 - ion to nitric oxide (NO) followed by binding with deoxyhemoglobin (HbFe2+) to form the yellowish green iron-nitrosyl-hemoglobin (HbFe2+-NO) complex. Fiber optic reflectance spectrophotometer was used to monitor the NO2 - ion concentration quantitatively based on colour change of the immobilized Hb on the polyacrylate microspheres from reddish brown to yellowish green at the maximum reflectance wavelength of 668 nm. Characterization of the reflectometric nitrite biosensor encompassed pH effect, Hb concentration effect, linear response range, reproducibility, shelf life and ion interference effect studies had been conducted. The developed optical NO2 - ion biosensor exhibited dynamic linear range from 5 to 50 mg mL-1 (R2=0.9894) at pH7.0 with a limit of detection (LOD) of 3.3 mg mL-1 and reproducibility relative standard deviation (RSD) value of the biosensor at 5.8%. The shelf life of the optical nitrite biosensor is about 36 day and majority foreign ions that are often co-exist with NO2 - ion in the natural water sample did not show significant interference effect towards the determination of NO2 - ion using the developed optical biosensor except Hg2+, Ag+, Br- and S2- ions.
AB - Optical biosensor based on hemoglobin (Hb) immobilized on the poly(n-butyl acrylate-co-N-acryloxysuccinimide) microspheres has been developed for the detection of nitrite (NO2 -) ion concentration. The iron HEM complex in the Hb catalyzed the reduction of NO2 - ion to nitric oxide (NO) followed by binding with deoxyhemoglobin (HbFe2+) to form the yellowish green iron-nitrosyl-hemoglobin (HbFe2+-NO) complex. Fiber optic reflectance spectrophotometer was used to monitor the NO2 - ion concentration quantitatively based on colour change of the immobilized Hb on the polyacrylate microspheres from reddish brown to yellowish green at the maximum reflectance wavelength of 668 nm. Characterization of the reflectometric nitrite biosensor encompassed pH effect, Hb concentration effect, linear response range, reproducibility, shelf life and ion interference effect studies had been conducted. The developed optical NO2 - ion biosensor exhibited dynamic linear range from 5 to 50 mg mL-1 (R2=0.9894) at pH7.0 with a limit of detection (LOD) of 3.3 mg mL-1 and reproducibility relative standard deviation (RSD) value of the biosensor at 5.8%. The shelf life of the optical nitrite biosensor is about 36 day and majority foreign ions that are often co-exist with NO2 - ion in the natural water sample did not show significant interference effect towards the determination of NO2 - ion using the developed optical biosensor except Hg2+, Ag+, Br- and S2- ions.
KW - Acrylic microsphere
KW - Biosensor
KW - Hemoglobin
KW - Nitrite determination
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UR - http://www.scopus.com/inward/citedby.url?scp=85056401476&partnerID=8YFLogxK
U2 - 10.17576/jsm-2018-4709-10
DO - 10.17576/jsm-2018-4709-10
M3 - Article
VL - 47
SP - 2027
EP - 2033
JO - Sains Malaysiana
JF - Sains Malaysiana
SN - 0126-6039
IS - 9
ER -