Optical biosensor test strip for the screening and direct determination of l-glutamate in food samples

Noor Zuhartini Md Muslim, Musa Ahmad, Yook Heng Lee, Bahruddin Saad

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

An optical glutamate biosensor test strip based on stacked membranes of nafion/sol-gel (bottom layer) and chitosan (uppermost layer) was fabricated on a piece of paper as support to form a test strip. The use of a stacked membrane system allows multiple immobilizations of sensing components directly without any covalent attachment via straight forward procedures. The uppermost membrane consisted of immobilised enzymes l-glutamate oxidase (GLOD) and horseradish peroxidase (HRP), which sensed the presence of l-glutamate and the bottom membrane contained the indicator dye, 3,3′,5,5′- tetramethylbenzidine. The test strip can be used to measure l-glutamate quantitatively by observing a colour change from light green to dark green with increasing l-glutamate concentrations. Quantitative analysis could be performed by measuring the reflectance intensity of the colour change at 550 nm. The glutamate biosensor test strip gave a linear response range of 0.01-0.30 mM to l-glutamate with a limit of detection of 5 μM. The strips were successfully applied for the estimation of l-glutamate in common food items such as sauces, soups, processed food and flavour enhancers. Results of analysis of l-glutamate in various food samples using the glutamate test strip were comparable to a standard procedure employing HPLC method.

Original languageEnglish
Pages (from-to)493-497
Number of pages5
JournalSensors and Actuators, B: Chemical
Volume161
Issue number1
DOIs
Publication statusPublished - 3 Jan 2012

Fingerprint

glutamates
bioinstrumentation
food
Biosensors
Glutamic Acid
strip
Screening
screening
Membranes
Processed foods
Color
Flavors
membranes
Chitosan
Sol-gels
Enzymes
Dyes
Methyl Green
color
Chemical analysis

Keywords

  • 3,3′,5,5′- Tetramethylbenzidine
  • Glutamate
  • Horseradish peroxidase
  • l-Glutamate oxidase
  • Optical glutamate biosensor
  • Test strip

ASJC Scopus subject areas

  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Optical biosensor test strip for the screening and direct determination of l-glutamate in food samples. / Md Muslim, Noor Zuhartini; Ahmad, Musa; Lee, Yook Heng; Saad, Bahruddin.

In: Sensors and Actuators, B: Chemical, Vol. 161, No. 1, 03.01.2012, p. 493-497.

Research output: Contribution to journalArticle

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