Optical biosensing using newly synthesized metal salphen complexes

A potential DNA diagnostic tool

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A solid-state DNA optode sensor has been developed based on the employment of glutaraldehyde (GD)-activated aminated silica nanoparticles (SiO2NPs-NH2) DNA carrier matrix, and the newly synthesized yellowish N,N’-bis-5-(hydroxysalicylidene)phenylene-diamine-zinc(II) (i.e. the Zn salphen complex) oligonucleotide label. Some three Schiff base complexes with three different transition metal ions (Zn2+, Ni2+, Cu2+) were synthesized through a simple one-pot reaction (5 h), and characterized by their physical and chemical properties based on FTIR, NMR and ESI–MS analyses. Due to the square planar geometry and aromatic ring structured characteristics of the metal complex intercalators, they could intercalate between DNA bases via π-π stacking interaction, and rendered a yellowish pink hue on the DNA biosensor surface. Reflectometric optimization revealed high selectivity of the nanosilica-based DNA biosensor in the detection of dengue serotype 2 DNA, which capable of discriminating even a single nucleotide mismatch. The optical DNA biosensor demonstrated a linear reflectance response between 1.0 × 10−15 M and 1.0 × 10−11 M cDNA (R2 = 0.9975) with a fast DNA hybridization time of 30 min and a limit of detectable (LOD) DNA concentration as low as 1 zM. In addition, this biosensor showed high shelf life stability with a 20-day operational duration and reusable for five consecutive DNA testings. The proposed optical DNA biosensor offers biosensing performance far superior to that of previously reported electrochemical DNA biosensor for early diagnosis of dengue infection with respect to dynamic linear range, LOD and response time.

Original languageEnglish
Pages (from-to)176-188
Number of pages13
JournalSensors and Actuators, B: Chemical
Volume242
DOIs
Publication statusPublished - 1 Apr 2017

Fingerprint

Metal complexes
DNA
deoxyribonucleic acid
bioinstrumentation
Biosensors
metals
salphen
Intercalating Agents
Diamines
Schiff Bases
oligonucleotides
Oligonucleotides
Coordination Complexes
nucleotides
Glutaral
infectious diseases
diamines
Nucleotides
Silicon Dioxide
chemical properties

Keywords

  • Aminated silica nanoparticles
  • DNA intercalator
  • Metal salphen
  • Optical DNA biosensor
  • Schiff base

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry
  • Electrical and Electronic Engineering

Cite this

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title = "Optical biosensing using newly synthesized metal salphen complexes: A potential DNA diagnostic tool",
abstract = "A solid-state DNA optode sensor has been developed based on the employment of glutaraldehyde (GD)-activated aminated silica nanoparticles (SiO2NPs-NH2) DNA carrier matrix, and the newly synthesized yellowish N,N’-bis-5-(hydroxysalicylidene)phenylene-diamine-zinc(II) (i.e. the Zn salphen complex) oligonucleotide label. Some three Schiff base complexes with three different transition metal ions (Zn2+, Ni2+, Cu2+) were synthesized through a simple one-pot reaction (5 h), and characterized by their physical and chemical properties based on FTIR, NMR and ESI–MS analyses. Due to the square planar geometry and aromatic ring structured characteristics of the metal complex intercalators, they could intercalate between DNA bases via π-π stacking interaction, and rendered a yellowish pink hue on the DNA biosensor surface. Reflectometric optimization revealed high selectivity of the nanosilica-based DNA biosensor in the detection of dengue serotype 2 DNA, which capable of discriminating even a single nucleotide mismatch. The optical DNA biosensor demonstrated a linear reflectance response between 1.0 × 10−15 M and 1.0 × 10−11 M cDNA (R2 = 0.9975) with a fast DNA hybridization time of 30 min and a limit of detectable (LOD) DNA concentration as low as 1 zM. In addition, this biosensor showed high shelf life stability with a 20-day operational duration and reusable for five consecutive DNA testings. The proposed optical DNA biosensor offers biosensing performance far superior to that of previously reported electrochemical DNA biosensor for early diagnosis of dengue infection with respect to dynamic linear range, LOD and response time.",
keywords = "Aminated silica nanoparticles, DNA intercalator, Metal salphen, Optical DNA biosensor, Schiff base",
author = "Mazlan, {Nur Fadhilah} and {Tan @ Chong}, {Ling Ling} and {Abd Karim}, {Nurul Huda} and Lee, {Yook Heng} and Reza, {Mohammad Imam Hasan}",
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T1 - Optical biosensing using newly synthesized metal salphen complexes

T2 - A potential DNA diagnostic tool

AU - Mazlan, Nur Fadhilah

AU - Tan @ Chong, Ling Ling

AU - Abd Karim, Nurul Huda

AU - Lee, Yook Heng

AU - Reza, Mohammad Imam Hasan

PY - 2017/4/1

Y1 - 2017/4/1

N2 - A solid-state DNA optode sensor has been developed based on the employment of glutaraldehyde (GD)-activated aminated silica nanoparticles (SiO2NPs-NH2) DNA carrier matrix, and the newly synthesized yellowish N,N’-bis-5-(hydroxysalicylidene)phenylene-diamine-zinc(II) (i.e. the Zn salphen complex) oligonucleotide label. Some three Schiff base complexes with three different transition metal ions (Zn2+, Ni2+, Cu2+) were synthesized through a simple one-pot reaction (5 h), and characterized by their physical and chemical properties based on FTIR, NMR and ESI–MS analyses. Due to the square planar geometry and aromatic ring structured characteristics of the metal complex intercalators, they could intercalate between DNA bases via π-π stacking interaction, and rendered a yellowish pink hue on the DNA biosensor surface. Reflectometric optimization revealed high selectivity of the nanosilica-based DNA biosensor in the detection of dengue serotype 2 DNA, which capable of discriminating even a single nucleotide mismatch. The optical DNA biosensor demonstrated a linear reflectance response between 1.0 × 10−15 M and 1.0 × 10−11 M cDNA (R2 = 0.9975) with a fast DNA hybridization time of 30 min and a limit of detectable (LOD) DNA concentration as low as 1 zM. In addition, this biosensor showed high shelf life stability with a 20-day operational duration and reusable for five consecutive DNA testings. The proposed optical DNA biosensor offers biosensing performance far superior to that of previously reported electrochemical DNA biosensor for early diagnosis of dengue infection with respect to dynamic linear range, LOD and response time.

AB - A solid-state DNA optode sensor has been developed based on the employment of glutaraldehyde (GD)-activated aminated silica nanoparticles (SiO2NPs-NH2) DNA carrier matrix, and the newly synthesized yellowish N,N’-bis-5-(hydroxysalicylidene)phenylene-diamine-zinc(II) (i.e. the Zn salphen complex) oligonucleotide label. Some three Schiff base complexes with three different transition metal ions (Zn2+, Ni2+, Cu2+) were synthesized through a simple one-pot reaction (5 h), and characterized by their physical and chemical properties based on FTIR, NMR and ESI–MS analyses. Due to the square planar geometry and aromatic ring structured characteristics of the metal complex intercalators, they could intercalate between DNA bases via π-π stacking interaction, and rendered a yellowish pink hue on the DNA biosensor surface. Reflectometric optimization revealed high selectivity of the nanosilica-based DNA biosensor in the detection of dengue serotype 2 DNA, which capable of discriminating even a single nucleotide mismatch. The optical DNA biosensor demonstrated a linear reflectance response between 1.0 × 10−15 M and 1.0 × 10−11 M cDNA (R2 = 0.9975) with a fast DNA hybridization time of 30 min and a limit of detectable (LOD) DNA concentration as low as 1 zM. In addition, this biosensor showed high shelf life stability with a 20-day operational duration and reusable for five consecutive DNA testings. The proposed optical DNA biosensor offers biosensing performance far superior to that of previously reported electrochemical DNA biosensor for early diagnosis of dengue infection with respect to dynamic linear range, LOD and response time.

KW - Aminated silica nanoparticles

KW - DNA intercalator

KW - Metal salphen

KW - Optical DNA biosensor

KW - Schiff base

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JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

SN - 0925-4005

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