Operation performance of up-flow anaerobic sludge blanket (UASB) bioreactor for biohydrogen production by self-granulated sludge using pre-treated palm oil mill effluent (POME) as carbon source

Safa Senan Mahmod, Azratul Madihah Azahar, Jian Ping Tan, Jamaliah Md Jahim, Peer Mohamed Abdul, Mohd Shahbudin Mastar @ Masdar, Nurina Anuar, Mohammed Faisal Mohammed Yunus, Ahmad Jaril Asis, Shu Yii Wu

Research output: Contribution to journalArticle

Abstract

Palm oil mill effluent (POME), an agro-industrial wastewater with high solids content, was subject to hydrolysis by 1% (w/v) nitric acid in order to increase its solubility and the fermentable sugar content from its cellulosic component. POME hydrolysate was then evaluated in an up-flow anaerobic sludge blanket (UASB) bioreactor for the production of biohydrogen gas via mixed culture under thermophilic conditions. The bioreactor was fed with pre-treated POME under varied hydraulic retention time (HRT) between 48 and 3 h at constant cycle length of 24 h to test the productivity of H2 and the stability of UASB; no washout of biomass occurred at any cycle and the system managed to recover its H2 production rate (HPR) after initial fluctuations. In this study, H2-producing granules (HPGs) were formed shortly after the start-up period, and were analysed by FESEM, FTIR, SEM-EDX, and their extracellular polymeric substances (EPS) content. The maximum HY and HPR achieved were 2.45 mol-H2/mol-sugar and 11.75 LH2/LPOME d−1, respectively, at HRT 6 h. Acetic acid was found to be the major by-product at all HRTs, followed by butyric acid, while Clostridium spp. was found to be the most dominant H2-producing bacteria in the system. Results suggest that UASB has a good potential for stable H2 production with high POME digestion rate.

Original languageEnglish
Pages (from-to)1262-1272
Number of pages11
JournalRenewable Energy
Volume134
DOIs
Publication statusPublished - 1 Apr 2019

Fingerprint

Palm oil
Sewage sludge
Bioreactors
Effluents
Carbon
Sugars
Hydraulics
Clostridium
Butyric acid
Nitric acid
Acetic acid
Byproducts
Energy dispersive spectroscopy
Hydrolysis
Bacteria
Biomass
Wastewater
Solubility
Productivity
Scanning electron microscopy

Keywords

  • Biohydrogen
  • H-producing granules
  • Hydraulic retention time
  • Palm oil mill effluent
  • UASB

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment

Cite this

Operation performance of up-flow anaerobic sludge blanket (UASB) bioreactor for biohydrogen production by self-granulated sludge using pre-treated palm oil mill effluent (POME) as carbon source. / Mahmod, Safa Senan; Azahar, Azratul Madihah; Tan, Jian Ping; Md Jahim, Jamaliah; Abdul, Peer Mohamed; Mastar @ Masdar, Mohd Shahbudin; Anuar, Nurina; Mohammed Yunus, Mohammed Faisal; Asis, Ahmad Jaril; Wu, Shu Yii.

In: Renewable Energy, Vol. 134, 01.04.2019, p. 1262-1272.

Research output: Contribution to journalArticle

Mahmod, Safa Senan ; Azahar, Azratul Madihah ; Tan, Jian Ping ; Md Jahim, Jamaliah ; Abdul, Peer Mohamed ; Mastar @ Masdar, Mohd Shahbudin ; Anuar, Nurina ; Mohammed Yunus, Mohammed Faisal ; Asis, Ahmad Jaril ; Wu, Shu Yii. / Operation performance of up-flow anaerobic sludge blanket (UASB) bioreactor for biohydrogen production by self-granulated sludge using pre-treated palm oil mill effluent (POME) as carbon source. In: Renewable Energy. 2019 ; Vol. 134. pp. 1262-1272.
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AU - Md Jahim, Jamaliah

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