Oil Palm Empty Fruit Bunch and Sugarcane Bagasse Enhance the Bioremediation of Soil Artificially Polluted by Crude Oil

Ainon Hamzah, Chia Wei Phan, Pek Hoon Yong, Nurul Hayati Mohd Ridzuan

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    Contamination of soil by petroleum hydrocarbons is becoming prevalent in Malaysia. Infiltration of soil contamination into groundwater poses a great threat to the ecosystem and human health. Bioremediation can occur naturally or can be enhanced with supplementation of microorganisms and fertilizers. However, fertilizers are expensive and therefore alternative nutrient-rich biomaterials are required. In this study, two organic wastes from agricultural industry (i.e., sugarcane bagasse and oil palm empty fruit bunch) were investigated for possible enhanced bioremediation of soil contaminated with Tapis crude oil. Two bacterial strains isolated and characterized previously (i.e., Pseudomonas aeruginosa UKMP-14T and Acinetobacter baumannii UKMP-12T) were used in this study. Sugarcane bagasse (5% and 15%, w/w) and oil palm empty fruit bunch (20%, w/w) were mixed with soil (500 g) spiked with Tapis crude oil (3%, v/w). The treated soils as well as controls were incubated for 20 days under controlled conditions. Sampling was carried out every four days to measure the number of bacterial colonies (CFU/g) and to determine the percentage of oil degradation by gas chromatography. The two biostimulating agents were able to maintain the soil moisture holding capacity, pH, and temperature at 38-40% volumetric moisture content (VMC), 7.0, and 29-30°C; respectively. The growth of bacteria consortium after 20 days in the treatment with sugarcane bagasse and oil palm empty fruit bunch had increased to 10.3 CFU/g and 9.5 CFU/g, respectively. The percentage of hydrocarbon degradation was higher in the soil amended with sugarcane bagasse (100%) when compared to that of oil palm empty fruit bunch (97%) after 20 days. Our results demonstrated the potential of sugarcane bagasse and oil palm empty fruit bunch as good substrates for enhanced bioremediation of soil contaminated with petroleum crude oil.

    Original languageEnglish
    Pages (from-to)751-762
    Number of pages12
    JournalSoil and Sediment Contamination
    Volume23
    Issue number7
    DOIs
    Publication statusPublished - 2014

    Fingerprint

    Saccharum
    sugarcane bagasse
    Bagasse
    Environmental Biodegradation
    Palm oil
    Bioremediation
    Elaeis guineensis
    bioremediation
    Petroleum
    Fruits
    polluted soils
    crude oil
    Fruit
    Oils
    Soil
    fruit
    Crude oil
    Soils
    oils
    fruits

    Keywords

    • agricultural waste
    • Bioremediation
    • crude oil
    • oil palm empty fruit bunch
    • soil contamination
    • sugarcane bagasse

    ASJC Scopus subject areas

    • Soil Science
    • Environmental Chemistry
    • Health, Toxicology and Mutagenesis
    • Pollution

    Cite this

    Oil Palm Empty Fruit Bunch and Sugarcane Bagasse Enhance the Bioremediation of Soil Artificially Polluted by Crude Oil. / Hamzah, Ainon; Phan, Chia Wei; Yong, Pek Hoon; Mohd Ridzuan, Nurul Hayati.

    In: Soil and Sediment Contamination, Vol. 23, No. 7, 2014, p. 751-762.

    Research output: Contribution to journalArticle

    Hamzah, Ainon ; Phan, Chia Wei ; Yong, Pek Hoon ; Mohd Ridzuan, Nurul Hayati. / Oil Palm Empty Fruit Bunch and Sugarcane Bagasse Enhance the Bioremediation of Soil Artificially Polluted by Crude Oil. In: Soil and Sediment Contamination. 2014 ; Vol. 23, No. 7. pp. 751-762.
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    abstract = "Contamination of soil by petroleum hydrocarbons is becoming prevalent in Malaysia. Infiltration of soil contamination into groundwater poses a great threat to the ecosystem and human health. Bioremediation can occur naturally or can be enhanced with supplementation of microorganisms and fertilizers. However, fertilizers are expensive and therefore alternative nutrient-rich biomaterials are required. In this study, two organic wastes from agricultural industry (i.e., sugarcane bagasse and oil palm empty fruit bunch) were investigated for possible enhanced bioremediation of soil contaminated with Tapis crude oil. Two bacterial strains isolated and characterized previously (i.e., Pseudomonas aeruginosa UKMP-14T and Acinetobacter baumannii UKMP-12T) were used in this study. Sugarcane bagasse (5{\%} and 15{\%}, w/w) and oil palm empty fruit bunch (20{\%}, w/w) were mixed with soil (500 g) spiked with Tapis crude oil (3{\%}, v/w). The treated soils as well as controls were incubated for 20 days under controlled conditions. Sampling was carried out every four days to measure the number of bacterial colonies (CFU/g) and to determine the percentage of oil degradation by gas chromatography. The two biostimulating agents were able to maintain the soil moisture holding capacity, pH, and temperature at 38-40{\%} volumetric moisture content (VMC), 7.0, and 29-30°C; respectively. The growth of bacteria consortium after 20 days in the treatment with sugarcane bagasse and oil palm empty fruit bunch had increased to 10.3 CFU/g and 9.5 CFU/g, respectively. The percentage of hydrocarbon degradation was higher in the soil amended with sugarcane bagasse (100{\%}) when compared to that of oil palm empty fruit bunch (97{\%}) after 20 days. Our results demonstrated the potential of sugarcane bagasse and oil palm empty fruit bunch as good substrates for enhanced bioremediation of soil contaminated with petroleum crude oil.",
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