Occurrence of Enterococcus species with virulence markers in an urban flow-influenced tropical recreational beach

Asmat Ahmad, Ayokunle Christopher Dada, Gires Usup, Yook Heng Lee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Median enterococci counts of beach water samples gradually increased at statistically significant levels (χ2: 26.53, df: 4; p<. 0.0001) with increasing proximity to river influx. The difference in proportion of antibiotic resistant enterococci in beach water and river water samples was statistically significant (p<. 0.05) for the tested antibiotics with river isolates generally presenting higher resistance frequencies. Virulence genes cyl, esp, gelE and asa were detected at varying frequencies (7.32%, 21.95%, 100% and 63.41% respectively) among river isolates. On the other hand, the prevalence of these genes was lower (0%, 20%, 67.27% and 41.82% respectively) among beach water isolates. Multi-Locus-Sequence-Typing analysis of Enterococcus faecalis presented four sequence types (ST) one of which shared six out of seven tested loci with ST6, a member of the clonal complex of multi-drug resistant strains associated with hospital outbreaks.

Original languageEnglish
Pages (from-to)26-38
Number of pages13
JournalMarine Pollution Bulletin
Volume82
Issue number1-2
DOIs
Publication statusPublished - 15 May 2014

Fingerprint

Enterococcus
Beaches
virulence
beaches
beach
Rivers
antibiotics
rivers
Antibiotics
river
Water
gene
Genes
water
Enterococcus faecalis
river water
drug
genes
sampling
drugs

Keywords

  • Antibiotic resistance
  • Beach water
  • Enterococcus
  • Sequence type

ASJC Scopus subject areas

  • Aquatic Science
  • Oceanography
  • Pollution

Cite this

Occurrence of Enterococcus species with virulence markers in an urban flow-influenced tropical recreational beach. / Ahmad, Asmat; Dada, Ayokunle Christopher; Usup, Gires; Lee, Yook Heng.

In: Marine Pollution Bulletin, Vol. 82, No. 1-2, 15.05.2014, p. 26-38.

Research output: Contribution to journalArticle

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abstract = "Median enterococci counts of beach water samples gradually increased at statistically significant levels (χ2: 26.53, df: 4; p<. 0.0001) with increasing proximity to river influx. The difference in proportion of antibiotic resistant enterococci in beach water and river water samples was statistically significant (p<. 0.05) for the tested antibiotics with river isolates generally presenting higher resistance frequencies. Virulence genes cyl, esp, gelE and asa were detected at varying frequencies (7.32{\%}, 21.95{\%}, 100{\%} and 63.41{\%} respectively) among river isolates. On the other hand, the prevalence of these genes was lower (0{\%}, 20{\%}, 67.27{\%} and 41.82{\%} respectively) among beach water isolates. Multi-Locus-Sequence-Typing analysis of Enterococcus faecalis presented four sequence types (ST) one of which shared six out of seven tested loci with ST6, a member of the clonal complex of multi-drug resistant strains associated with hospital outbreaks.",
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