Occurrence and molecular characterisation of Acanthamoeba isolated from recreational hot springs in Malaysia: evidence of pathogenic potential

Rosnani Hanim Mohd Hussain, Ahmad Razali Ishak, Mohamed Kamel Abdul Ghani, Naveed Ahmed Khan, Ruqaiyyah Siddiqui, Tengku Shahrul Anuar

Research output: Contribution to journalArticle

Abstract

This study aimed to identify the Acanthamoeba genotypes and their pathogenic potential in five recreational hot springs in Peninsular Malaysia. Fifty water samples were collected between April and September 2018. Physical parameters of water quality were measured in situ while chemical and microbiological analyses were performed in the laboratory. All samples were filtered through the nitrocellulose membrane and tested for Acanthamoeba using both cultivation and polymerase chain reaction (PCR) by targeting the 18S ribosomal RNA gene. The pathogenic potential of all positive isolates was identified using thermo- and osmotolerance tests. Thirty-eight (76.0%) samples were positive for Acanthamoeba. Water temperature (P = 0.035), chemical oxygen demand (P = 0.026), sulphate (P = 0.002) and Escherichia coli (P < 0.001) were found to be significantly correlated with the presence of Acanthamoeba. Phylogenetic analysis revealed that 24 samples belonged to genotype T4, nine (T15), two (T3) and one from each genotype T5, T11 and T17. Thermo- and osmotolerance tests showed that 6 (15.79%) of the Acanthamoeba strains were highly pathogenic. The existence of Acanthamoeba in recreational hot springs should be considered as a health threat among the public especially for high-risk people. Periodic surveillance of hot spring waters and posting warning signs by health authorities is recommended to prevent disease related to pathogenic Acanthamoeba.

Original languageEnglish
Pages (from-to)813-825
Number of pages13
JournalJournal of water and health
Volume17
Issue number5
DOIs
Publication statusPublished - 1 Oct 2019

Fingerprint

Hot Springs
Acanthamoeba
Malaysia
thermal spring
genotype
spring water
Genotype
chemical oxygen demand
polymerase chain reaction
targeting
RNA
water temperature
Water
sulfate
membrane
phylogenetics
water quality
18S Ribosomal RNA
gene
Biological Oxygen Demand Analysis

ASJC Scopus subject areas

  • Water Science and Technology
  • Waste Management and Disposal
  • Public Health, Environmental and Occupational Health
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Occurrence and molecular characterisation of Acanthamoeba isolated from recreational hot springs in Malaysia : evidence of pathogenic potential. / Mohd Hussain, Rosnani Hanim; Ishak, Ahmad Razali; Abdul Ghani, Mohamed Kamel; Ahmed Khan, Naveed; Siddiqui, Ruqaiyyah; Shahrul Anuar, Tengku.

In: Journal of water and health, Vol. 17, No. 5, 01.10.2019, p. 813-825.

Research output: Contribution to journalArticle

Mohd Hussain, Rosnani Hanim ; Ishak, Ahmad Razali ; Abdul Ghani, Mohamed Kamel ; Ahmed Khan, Naveed ; Siddiqui, Ruqaiyyah ; Shahrul Anuar, Tengku. / Occurrence and molecular characterisation of Acanthamoeba isolated from recreational hot springs in Malaysia : evidence of pathogenic potential. In: Journal of water and health. 2019 ; Vol. 17, No. 5. pp. 813-825.
@article{53418aa1da0141dcb9d26449ecad4f4e,
title = "Occurrence and molecular characterisation of Acanthamoeba isolated from recreational hot springs in Malaysia: evidence of pathogenic potential",
abstract = "This study aimed to identify the Acanthamoeba genotypes and their pathogenic potential in five recreational hot springs in Peninsular Malaysia. Fifty water samples were collected between April and September 2018. Physical parameters of water quality were measured in situ while chemical and microbiological analyses were performed in the laboratory. All samples were filtered through the nitrocellulose membrane and tested for Acanthamoeba using both cultivation and polymerase chain reaction (PCR) by targeting the 18S ribosomal RNA gene. The pathogenic potential of all positive isolates was identified using thermo- and osmotolerance tests. Thirty-eight (76.0{\%}) samples were positive for Acanthamoeba. Water temperature (P = 0.035), chemical oxygen demand (P = 0.026), sulphate (P = 0.002) and Escherichia coli (P < 0.001) were found to be significantly correlated with the presence of Acanthamoeba. Phylogenetic analysis revealed that 24 samples belonged to genotype T4, nine (T15), two (T3) and one from each genotype T5, T11 and T17. Thermo- and osmotolerance tests showed that 6 (15.79{\%}) of the Acanthamoeba strains were highly pathogenic. The existence of Acanthamoeba in recreational hot springs should be considered as a health threat among the public especially for high-risk people. Periodic surveillance of hot spring waters and posting warning signs by health authorities is recommended to prevent disease related to pathogenic Acanthamoeba.",
author = "{Mohd Hussain}, {Rosnani Hanim} and Ishak, {Ahmad Razali} and {Abdul Ghani}, {Mohamed Kamel} and {Ahmed Khan}, Naveed and Ruqaiyyah Siddiqui and {Shahrul Anuar}, Tengku",
year = "2019",
month = "10",
day = "1",
doi = "10.2166/wh.2019.214",
language = "English",
volume = "17",
pages = "813--825",
journal = "Journal of Water and Health",
issn = "1477-8920",
publisher = "IWA Publishing",
number = "5",

}

TY - JOUR

T1 - Occurrence and molecular characterisation of Acanthamoeba isolated from recreational hot springs in Malaysia

T2 - evidence of pathogenic potential

AU - Mohd Hussain, Rosnani Hanim

AU - Ishak, Ahmad Razali

AU - Abdul Ghani, Mohamed Kamel

AU - Ahmed Khan, Naveed

AU - Siddiqui, Ruqaiyyah

AU - Shahrul Anuar, Tengku

PY - 2019/10/1

Y1 - 2019/10/1

N2 - This study aimed to identify the Acanthamoeba genotypes and their pathogenic potential in five recreational hot springs in Peninsular Malaysia. Fifty water samples were collected between April and September 2018. Physical parameters of water quality were measured in situ while chemical and microbiological analyses were performed in the laboratory. All samples were filtered through the nitrocellulose membrane and tested for Acanthamoeba using both cultivation and polymerase chain reaction (PCR) by targeting the 18S ribosomal RNA gene. The pathogenic potential of all positive isolates was identified using thermo- and osmotolerance tests. Thirty-eight (76.0%) samples were positive for Acanthamoeba. Water temperature (P = 0.035), chemical oxygen demand (P = 0.026), sulphate (P = 0.002) and Escherichia coli (P < 0.001) were found to be significantly correlated with the presence of Acanthamoeba. Phylogenetic analysis revealed that 24 samples belonged to genotype T4, nine (T15), two (T3) and one from each genotype T5, T11 and T17. Thermo- and osmotolerance tests showed that 6 (15.79%) of the Acanthamoeba strains were highly pathogenic. The existence of Acanthamoeba in recreational hot springs should be considered as a health threat among the public especially for high-risk people. Periodic surveillance of hot spring waters and posting warning signs by health authorities is recommended to prevent disease related to pathogenic Acanthamoeba.

AB - This study aimed to identify the Acanthamoeba genotypes and their pathogenic potential in five recreational hot springs in Peninsular Malaysia. Fifty water samples were collected between April and September 2018. Physical parameters of water quality were measured in situ while chemical and microbiological analyses were performed in the laboratory. All samples were filtered through the nitrocellulose membrane and tested for Acanthamoeba using both cultivation and polymerase chain reaction (PCR) by targeting the 18S ribosomal RNA gene. The pathogenic potential of all positive isolates was identified using thermo- and osmotolerance tests. Thirty-eight (76.0%) samples were positive for Acanthamoeba. Water temperature (P = 0.035), chemical oxygen demand (P = 0.026), sulphate (P = 0.002) and Escherichia coli (P < 0.001) were found to be significantly correlated with the presence of Acanthamoeba. Phylogenetic analysis revealed that 24 samples belonged to genotype T4, nine (T15), two (T3) and one from each genotype T5, T11 and T17. Thermo- and osmotolerance tests showed that 6 (15.79%) of the Acanthamoeba strains were highly pathogenic. The existence of Acanthamoeba in recreational hot springs should be considered as a health threat among the public especially for high-risk people. Periodic surveillance of hot spring waters and posting warning signs by health authorities is recommended to prevent disease related to pathogenic Acanthamoeba.

UR - http://www.scopus.com/inward/record.url?scp=85073655461&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85073655461&partnerID=8YFLogxK

U2 - 10.2166/wh.2019.214

DO - 10.2166/wh.2019.214

M3 - Article

C2 - 31638031

AN - SCOPUS:85073655461

VL - 17

SP - 813

EP - 825

JO - Journal of Water and Health

JF - Journal of Water and Health

SN - 1477-8920

IS - 5

ER -