Observation of dendritic cell morphology under light, phase-contrast or confocal laser scanning microscopy

Yuen Fen Tan, Leong Chooi Fun, Soon Keng Cheong

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Dendritic cells (DCs) are professional antigen presenting cells of the immune system. They can be generated in vitro from peripheral blood monocytes supplemented with GM-CSF, IL-4 and TNF alpha. During induction, DCs will increase in size and acquire multiple cytoplasmic projections when compared to their precursor cells such as monocytes or haematopoietic stem cells which are usually round or spherical. Morphology of DCs can be visualized by conventional light microscopy after staining or phase-contrast inverted microscopy or confocal laser scanning microscopy. In this report, we described the morphological appearances of DCs captured using the above-mentioned techniques. We found that confocal laser scanning microscopy yielded DCs images with greater details but the operating cost for such a technique is high. On the other hand, the images obtained through light microscopy after appropriate staining or phase contrast microscopy were acceptable for identifi cation purpose. Besides, these equipments are readily available in most laboratories and the cost of operation is affordable. Nevertheless, morphological identifi cation is just one of the methods to characterise DCs. Other methods such as phenotypic expression markers and mixed leukocyte reactions are additional tools used in the characterisation of DCs.

Original languageEnglish
Pages (from-to)97-102
Number of pages6
JournalMalaysian Journal of Pathology
Volume32
Issue number2
Publication statusPublished - Dec 2010

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Confocal Microscopy
Dendritic Cells
Observation
Light
Phase-Contrast Microscopy
Cations
Monocytes
Microscopy
Staining and Labeling
Costs and Cost Analysis
Mixed Lymphocyte Culture Test
Antigen-Presenting Cells
Granulocyte-Macrophage Colony-Stimulating Factor
Hematopoietic Stem Cells
Interleukin-4
Immune System
Tumor Necrosis Factor-alpha
Equipment and Supplies

Keywords

  • Confocal laser scanning microscope
  • Dendritic cells
  • Inverted phase contrast microscope
  • Light microscope

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Histology

Cite this

Observation of dendritic cell morphology under light, phase-contrast or confocal laser scanning microscopy. / Tan, Yuen Fen; Chooi Fun, Leong; Cheong, Soon Keng.

In: Malaysian Journal of Pathology, Vol. 32, No. 2, 12.2010, p. 97-102.

Research output: Contribution to journalArticle

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