Nucleotide variability in the 5-enolpyruvylshikiniate-3-phosphate synthase gene from Eleusine indica (L.) Gaertn

J. L. Chong, R Wickneswari V Ratnam, Ismail Sahid, S. Salmijah

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This study reports the results of the partial DNA sequence analysis of the 5-enolpyruvyl-shikimate-3-phosphate synthase (EPSPS) gene in glyphosate-resistant (R) and glyphosate-susceptible (S) biotypes of Eleusine indica (L.) Gaertn from Peninsular Malaysia. Sequencing results revealed point mutation at nucleotide position 875 in the R biotypes of Bidor, Chaah and Temerloh. In the Chaah R population, substitution of cytosine (C) to adenine (A) resulted in the change of threonine (Thr106) to proline (Pro 106) and from C to fhymidine (T) in the Bidor R population, leading to serine (Ser106) from Pro106 As for the Temerloh R, C was substituted by T resulting in the change of Pro106. to Ser 106. A new mutation previously undetected in the Temerloh R was revealed with C being substituted with A, resulting in the change of Pro 106 to Thr106 indicating multiple founding events rather than to the spread of a single resistant allele. There was no point mutation recorded at nucleotide position 875 previously demonstrated to play a pivotal role in conferring glyphosate resistance to E. indica for the Lenggeng, Kuala Selangor, Melaka R populations. Thus, there may be another resistance mechanism yet undiscovered in the resistant Lenggeng, Kuala Selangor and Melaka populations.

Original languageEnglish
Pages (from-to)476-479
Number of pages4
JournalPakistan Journal of Biological Sciences
Volume11
Issue number3
DOIs
Publication statusPublished - 2008

Fingerprint

Eleusine indica
glyphosate
nucleotides
phosphates
point mutation
biotypes
genes
cytosine
adenine
resistance mechanisms
threonine
Malaysia
serine
proline
sequence analysis
alleles
mutation
nucleotide sequences

Keywords

  • DNA sequence analysis
  • Glyphosate- susceptible
  • Glyphosate-resistance
  • Goosegrass
  • Malaysia

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

@article{bae280aa38504d908d68bda8932b48ab,
title = "Nucleotide variability in the 5-enolpyruvylshikiniate-3-phosphate synthase gene from Eleusine indica (L.) Gaertn",
abstract = "This study reports the results of the partial DNA sequence analysis of the 5-enolpyruvyl-shikimate-3-phosphate synthase (EPSPS) gene in glyphosate-resistant (R) and glyphosate-susceptible (S) biotypes of Eleusine indica (L.) Gaertn from Peninsular Malaysia. Sequencing results revealed point mutation at nucleotide position 875 in the R biotypes of Bidor, Chaah and Temerloh. In the Chaah R population, substitution of cytosine (C) to adenine (A) resulted in the change of threonine (Thr106) to proline (Pro 106) and from C to fhymidine (T) in the Bidor R population, leading to serine (Ser106) from Pro106 As for the Temerloh R, C was substituted by T resulting in the change of Pro106. to Ser 106. A new mutation previously undetected in the Temerloh R was revealed with C being substituted with A, resulting in the change of Pro 106 to Thr106 indicating multiple founding events rather than to the spread of a single resistant allele. There was no point mutation recorded at nucleotide position 875 previously demonstrated to play a pivotal role in conferring glyphosate resistance to E. indica for the Lenggeng, Kuala Selangor, Melaka R populations. Thus, there may be another resistance mechanism yet undiscovered in the resistant Lenggeng, Kuala Selangor and Melaka populations.",
keywords = "DNA sequence analysis, Glyphosate- susceptible, Glyphosate-resistance, Goosegrass, Malaysia",
author = "Chong, {J. L.} and {V Ratnam}, {R Wickneswari} and Ismail Sahid and S. Salmijah",
year = "2008",
doi = "10.3923/pjbs.2008.476.479",
language = "English",
volume = "11",
pages = "476--479",
journal = "Pakistan Journal of Biological Sciences",
issn = "1028-8880",
publisher = "Asian Network for Scientific Information",
number = "3",

}

TY - JOUR

T1 - Nucleotide variability in the 5-enolpyruvylshikiniate-3-phosphate synthase gene from Eleusine indica (L.) Gaertn

AU - Chong, J. L.

AU - V Ratnam, R Wickneswari

AU - Sahid, Ismail

AU - Salmijah, S.

PY - 2008

Y1 - 2008

N2 - This study reports the results of the partial DNA sequence analysis of the 5-enolpyruvyl-shikimate-3-phosphate synthase (EPSPS) gene in glyphosate-resistant (R) and glyphosate-susceptible (S) biotypes of Eleusine indica (L.) Gaertn from Peninsular Malaysia. Sequencing results revealed point mutation at nucleotide position 875 in the R biotypes of Bidor, Chaah and Temerloh. In the Chaah R population, substitution of cytosine (C) to adenine (A) resulted in the change of threonine (Thr106) to proline (Pro 106) and from C to fhymidine (T) in the Bidor R population, leading to serine (Ser106) from Pro106 As for the Temerloh R, C was substituted by T resulting in the change of Pro106. to Ser 106. A new mutation previously undetected in the Temerloh R was revealed with C being substituted with A, resulting in the change of Pro 106 to Thr106 indicating multiple founding events rather than to the spread of a single resistant allele. There was no point mutation recorded at nucleotide position 875 previously demonstrated to play a pivotal role in conferring glyphosate resistance to E. indica for the Lenggeng, Kuala Selangor, Melaka R populations. Thus, there may be another resistance mechanism yet undiscovered in the resistant Lenggeng, Kuala Selangor and Melaka populations.

AB - This study reports the results of the partial DNA sequence analysis of the 5-enolpyruvyl-shikimate-3-phosphate synthase (EPSPS) gene in glyphosate-resistant (R) and glyphosate-susceptible (S) biotypes of Eleusine indica (L.) Gaertn from Peninsular Malaysia. Sequencing results revealed point mutation at nucleotide position 875 in the R biotypes of Bidor, Chaah and Temerloh. In the Chaah R population, substitution of cytosine (C) to adenine (A) resulted in the change of threonine (Thr106) to proline (Pro 106) and from C to fhymidine (T) in the Bidor R population, leading to serine (Ser106) from Pro106 As for the Temerloh R, C was substituted by T resulting in the change of Pro106. to Ser 106. A new mutation previously undetected in the Temerloh R was revealed with C being substituted with A, resulting in the change of Pro 106 to Thr106 indicating multiple founding events rather than to the spread of a single resistant allele. There was no point mutation recorded at nucleotide position 875 previously demonstrated to play a pivotal role in conferring glyphosate resistance to E. indica for the Lenggeng, Kuala Selangor, Melaka R populations. Thus, there may be another resistance mechanism yet undiscovered in the resistant Lenggeng, Kuala Selangor and Melaka populations.

KW - DNA sequence analysis

KW - Glyphosate- susceptible

KW - Glyphosate-resistance

KW - Goosegrass

KW - Malaysia

UR - http://www.scopus.com/inward/record.url?scp=53649101925&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=53649101925&partnerID=8YFLogxK

U2 - 10.3923/pjbs.2008.476.479

DO - 10.3923/pjbs.2008.476.479

M3 - Article

VL - 11

SP - 476

EP - 479

JO - Pakistan Journal of Biological Sciences

JF - Pakistan Journal of Biological Sciences

SN - 1028-8880

IS - 3

ER -