Nucleotide sequence of the coat protein gene of the Malaysian Passiflora Virus and its 3' non-coding region

Norzihan Abdullah, Ismanizan Ismail, Vilasini Pillai, Ruslan Abdullah, Shaiful Adzni Sharifudin

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Problem statement: In this study, we identified the full length Coat Protein (CP) gene of the Malaysian Passiflora Virus (MPV) and its 3' non-coding region. The CP gene of the MPV contained 285 amino acid residues. Approach: Pairwise comparison of the MPV CP region with four other potyviruses, namely East Asian Passiflora Virus (EAPV), Passionfruit Woodiness Virus (PWV), Bean Common Mosaic Virus (BCMV) and Soyabean Mosaic Virus (SMV) revealed amino acid sequence similarities ranging from 72-95%. Results: The 3' non-coding region of the MPV, which consists of 255 nucleotides, showed 69-95% nucleotide sequence identity when compared with the four potyviruses. The highest (95%) sequence similarities were detected with PWV and EAPV. An analysis of the deduced amino acid sequences revealed the presence of consensus motifs (DAG tripeptides) characteristic of potyviruses. DAG tripeptides had been reported to be essential for aphid transmission. Conclusion: From the amino acid sequence alignment and identity level observed among the four other potyviruses, we concluded that MPV is a member of the genus Potyvirus and was closely related to both PWV and EAPV.

Original languageEnglish
Pages (from-to)1633-1636
Number of pages4
JournalAmerican Journal of Applied Sciences
Volume6
Issue number9
DOIs
Publication statusPublished - 2009

Fingerprint

Passiflora
Potyviridae
Passion fruit woodiness virus
coat proteins
nucleotide sequences
viruses
tripeptides
amino acid sequences
genes
Soybean mosaic virus
Bean common mosaic virus
Potyvirus
sequence alignment
Aphidoidea
nucleotides
amino acids
East Asian Passiflora virus

Keywords

  • Coat protein
  • Passionfruit
  • Plant virus
  • Potyviruses

ASJC Scopus subject areas

  • General

Cite this

Nucleotide sequence of the coat protein gene of the Malaysian Passiflora Virus and its 3' non-coding region. / Abdullah, Norzihan; Ismail, Ismanizan; Pillai, Vilasini; Abdullah, Ruslan; Sharifudin, Shaiful Adzni.

In: American Journal of Applied Sciences, Vol. 6, No. 9, 2009, p. 1633-1636.

Research output: Contribution to journalArticle

Abdullah, Norzihan ; Ismail, Ismanizan ; Pillai, Vilasini ; Abdullah, Ruslan ; Sharifudin, Shaiful Adzni. / Nucleotide sequence of the coat protein gene of the Malaysian Passiflora Virus and its 3' non-coding region. In: American Journal of Applied Sciences. 2009 ; Vol. 6, No. 9. pp. 1633-1636.
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