Abstract
Problem statement: In this study, we identified the full length Coat Protein (CP) gene of the Malaysian Passiflora Virus (MPV) and its 3' non-coding region. The CP gene of the MPV contained 285 amino acid residues. Approach: Pairwise comparison of the MPV CP region with four other potyviruses, namely East Asian Passiflora Virus (EAPV), Passionfruit Woodiness Virus (PWV), Bean Common Mosaic Virus (BCMV) and Soyabean Mosaic Virus (SMV) revealed amino acid sequence similarities ranging from 72-95%. Results: The 3' non-coding region of the MPV, which consists of 255 nucleotides, showed 69-95% nucleotide sequence identity when compared with the four potyviruses. The highest (95%) sequence similarities were detected with PWV and EAPV. An analysis of the deduced amino acid sequences revealed the presence of consensus motifs (DAG tripeptides) characteristic of potyviruses. DAG tripeptides had been reported to be essential for aphid transmission. Conclusion: From the amino acid sequence alignment and identity level observed among the four other potyviruses, we concluded that MPV is a member of the genus Potyvirus and was closely related to both PWV and EAPV.
| Original language | English |
|---|---|
| Pages (from-to) | 1633-1636 |
| Number of pages | 4 |
| Journal | American Journal of Applied Sciences |
| Volume | 6 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2009 |
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Keywords
- Coat protein
- Passionfruit
- Plant virus
- Potyviruses
ASJC Scopus subject areas
- General
Cite this
Nucleotide sequence of the coat protein gene of the Malaysian Passiflora Virus and its 3' non-coding region. / Abdullah, Norzihan; Ismail, Ismanizan; Pillai, Vilasini; Abdullah, Ruslan; Sharifudin, Shaiful Adzni.
In: American Journal of Applied Sciences, Vol. 6, No. 9, 2009, p. 1633-1636.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nucleotide sequence of the coat protein gene of the Malaysian Passiflora Virus and its 3' non-coding region
AU - Abdullah, Norzihan
AU - Ismail, Ismanizan
AU - Pillai, Vilasini
AU - Abdullah, Ruslan
AU - Sharifudin, Shaiful Adzni
PY - 2009
Y1 - 2009
N2 - Problem statement: In this study, we identified the full length Coat Protein (CP) gene of the Malaysian Passiflora Virus (MPV) and its 3' non-coding region. The CP gene of the MPV contained 285 amino acid residues. Approach: Pairwise comparison of the MPV CP region with four other potyviruses, namely East Asian Passiflora Virus (EAPV), Passionfruit Woodiness Virus (PWV), Bean Common Mosaic Virus (BCMV) and Soyabean Mosaic Virus (SMV) revealed amino acid sequence similarities ranging from 72-95%. Results: The 3' non-coding region of the MPV, which consists of 255 nucleotides, showed 69-95% nucleotide sequence identity when compared with the four potyviruses. The highest (95%) sequence similarities were detected with PWV and EAPV. An analysis of the deduced amino acid sequences revealed the presence of consensus motifs (DAG tripeptides) characteristic of potyviruses. DAG tripeptides had been reported to be essential for aphid transmission. Conclusion: From the amino acid sequence alignment and identity level observed among the four other potyviruses, we concluded that MPV is a member of the genus Potyvirus and was closely related to both PWV and EAPV.
AB - Problem statement: In this study, we identified the full length Coat Protein (CP) gene of the Malaysian Passiflora Virus (MPV) and its 3' non-coding region. The CP gene of the MPV contained 285 amino acid residues. Approach: Pairwise comparison of the MPV CP region with four other potyviruses, namely East Asian Passiflora Virus (EAPV), Passionfruit Woodiness Virus (PWV), Bean Common Mosaic Virus (BCMV) and Soyabean Mosaic Virus (SMV) revealed amino acid sequence similarities ranging from 72-95%. Results: The 3' non-coding region of the MPV, which consists of 255 nucleotides, showed 69-95% nucleotide sequence identity when compared with the four potyviruses. The highest (95%) sequence similarities were detected with PWV and EAPV. An analysis of the deduced amino acid sequences revealed the presence of consensus motifs (DAG tripeptides) characteristic of potyviruses. DAG tripeptides had been reported to be essential for aphid transmission. Conclusion: From the amino acid sequence alignment and identity level observed among the four other potyviruses, we concluded that MPV is a member of the genus Potyvirus and was closely related to both PWV and EAPV.
KW - Coat protein
KW - Passionfruit
KW - Plant virus
KW - Potyviruses
UR - http://www.scopus.com/inward/record.url?scp=70350020510&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70350020510&partnerID=8YFLogxK
U2 - 10.3844/ajassp.2009.1633.1636
DO - 10.3844/ajassp.2009.1633.1636
M3 - Article
AN - SCOPUS:70350020510
VL - 6
SP - 1633
EP - 1636
JO - American Journal of Applied Sciences
JF - American Journal of Applied Sciences
SN - 1546-9239
IS - 9
ER -