Nuclear interaction of EGFR and STAT3 in the activation of the iNOS/NO pathway

Hui Wen Lo, Sheng Chieh Hsu, Mohamed Ali-Seyed, Mehmet Gunduz, Weiya Xia, Yongkun Wei, Geoffrey Bartholomeusz, Jin Yuan Shih, Mien Chie Hung

Research output: Contribution to journalArticle

341 Citations (Scopus)

Abstract

Epidermal growth factor receptor (EGFR) exists in the nucleus of highly proliferative cells where it functions as a transcription factor. Although EGFR has transactivational activity, it lacks a DNA binding domain and, therefore, may require a DNA binding transcription cofactor for its transcriptional function. Here, we report that EGFR physically interacts with signal transducers and activators of transcription 3 (STAT3) in the nucleus, leading to transcriptional activation of inducible nitric oxide synthase (iNOS). In breast carcinomas, nuclear EGFR positively correlates with iNOS. This study describes a mode of transcriptional control involving cooperated efforts of STAT3 and nuclear EGFR. Our work suggests that the deregulated iNOS/NO pathway may partly contribute to the malignant biology of tumor cells with high levels of nuclear EGFR and STAT3.

Original languageEnglish
Pages (from-to)575-589
Number of pages15
JournalCancer Cell
Volume7
Issue number6
DOIs
Publication statusPublished - Jun 2005
Externally publishedYes

Fingerprint

STAT3 Transcription Factor
Nitric Oxide Synthase Type II
Epidermal Growth Factor Receptor
DNA
Transcriptional Activation
Cell Biology
Transcription Factors
Breast Neoplasms
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Oncology

Cite this

Lo, H. W., Hsu, S. C., Ali-Seyed, M., Gunduz, M., Xia, W., Wei, Y., ... Hung, M. C. (2005). Nuclear interaction of EGFR and STAT3 in the activation of the iNOS/NO pathway. Cancer Cell, 7(6), 575-589. https://doi.org/10.1016/j.ccr.2005.05.007

Nuclear interaction of EGFR and STAT3 in the activation of the iNOS/NO pathway. / Lo, Hui Wen; Hsu, Sheng Chieh; Ali-Seyed, Mohamed; Gunduz, Mehmet; Xia, Weiya; Wei, Yongkun; Bartholomeusz, Geoffrey; Shih, Jin Yuan; Hung, Mien Chie.

In: Cancer Cell, Vol. 7, No. 6, 06.2005, p. 575-589.

Research output: Contribution to journalArticle

Lo, HW, Hsu, SC, Ali-Seyed, M, Gunduz, M, Xia, W, Wei, Y, Bartholomeusz, G, Shih, JY & Hung, MC 2005, 'Nuclear interaction of EGFR and STAT3 in the activation of the iNOS/NO pathway', Cancer Cell, vol. 7, no. 6, pp. 575-589. https://doi.org/10.1016/j.ccr.2005.05.007
Lo, Hui Wen ; Hsu, Sheng Chieh ; Ali-Seyed, Mohamed ; Gunduz, Mehmet ; Xia, Weiya ; Wei, Yongkun ; Bartholomeusz, Geoffrey ; Shih, Jin Yuan ; Hung, Mien Chie. / Nuclear interaction of EGFR and STAT3 in the activation of the iNOS/NO pathway. In: Cancer Cell. 2005 ; Vol. 7, No. 6. pp. 575-589.
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