NRESTdb: Access to the transcriptome of natural rubber latex

Mohd Noor Mat-Isa, Keng See Chow, Ahmad Fuad Hilmi Mohamad, Mohd Yunus Shahrum, Chee Chong Hoh, Mohd Rashdi Mohd-Amin, Khairil Anuar Zainal, Hoong Yeet Yeang, Kiew Lian Wan

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Current technological advancement of DNA sequencing has afforded opportunities to analyse genes on a larger scale. In natural rubber research, DNA sequencing technology has been used in the generation of approximately 10,000 DNA sequences representing genes expressed in the latex of Hevea brasiliensis (Muell. Arg.) clone RRIM600. These sequence data, more popularly known as expressed sequence tags (ESTs), can be analysed using bioinformatics tools to facilitate gene discovery and provide information regarding the putative function and expression profile of latex genes. We have developed NRESTdb, a Natural Rubber EST database, to provide easy access and rapid analysis of these data. To our knowledge, this is the first publicly available EST database for H. brasiliensis. In addition to serving as a repository for individual latex EST and clustered sequences, this database allows users to view, mine and retrieve useful information regarding: the transcriptome of natural rubber latex including matches with existing domains and gene sequences available in public databases; description based on the Gene Ontology nomenclature; homologues identified via comparative sequence analysis; and enhanced data sets of special interest, namely rubber biosynthesis and latex allergy.

Original languageEnglish
Pages (from-to)229-238
Number of pages10
JournalJournal of Rubber Research
Volume12
Issue number4
Publication statusPublished - 2009

Fingerprint

Rubber
Latex
latex
rubber
transcriptome
expressed sequence tags
Genes
Expressed Sequence Tags
Hevea brasiliensis
sequence analysis
genes
nucleotide sequences
Allergies
bioinformatics
hypersensitivity
DNA sequences
DNA
Biosynthesis
Bioinformatics
Terminology

Keywords

  • Bioinformatics
  • Database
  • EST
  • Genomics
  • Hevea brasiliensis
  • Transcriptome
  • Unique transcript

ASJC Scopus subject areas

  • Plant Science
  • Organic Chemistry

Cite this

Mat-Isa, M. N., Chow, K. S., Mohamad, A. F. H., Shahrum, M. Y., Hoh, C. C., Mohd-Amin, M. R., ... Wan, K. L. (2009). NRESTdb: Access to the transcriptome of natural rubber latex. Journal of Rubber Research, 12(4), 229-238.

NRESTdb : Access to the transcriptome of natural rubber latex. / Mat-Isa, Mohd Noor; Chow, Keng See; Mohamad, Ahmad Fuad Hilmi; Shahrum, Mohd Yunus; Hoh, Chee Chong; Mohd-Amin, Mohd Rashdi; Zainal, Khairil Anuar; Yeang, Hoong Yeet; Wan, Kiew Lian.

In: Journal of Rubber Research, Vol. 12, No. 4, 2009, p. 229-238.

Research output: Contribution to journalArticle

Mat-Isa, MN, Chow, KS, Mohamad, AFH, Shahrum, MY, Hoh, CC, Mohd-Amin, MR, Zainal, KA, Yeang, HY & Wan, KL 2009, 'NRESTdb: Access to the transcriptome of natural rubber latex', Journal of Rubber Research, vol. 12, no. 4, pp. 229-238.
Mat-Isa MN, Chow KS, Mohamad AFH, Shahrum MY, Hoh CC, Mohd-Amin MR et al. NRESTdb: Access to the transcriptome of natural rubber latex. Journal of Rubber Research. 2009;12(4):229-238.
Mat-Isa, Mohd Noor ; Chow, Keng See ; Mohamad, Ahmad Fuad Hilmi ; Shahrum, Mohd Yunus ; Hoh, Chee Chong ; Mohd-Amin, Mohd Rashdi ; Zainal, Khairil Anuar ; Yeang, Hoong Yeet ; Wan, Kiew Lian. / NRESTdb : Access to the transcriptome of natural rubber latex. In: Journal of Rubber Research. 2009 ; Vol. 12, No. 4. pp. 229-238.
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