Novel ripening-specific cdna clones from mango fruit

Grantley W. Lycelt, Zamri Zainal, Martin Los, Caroline L. Findlay, Gregory A. Tucker

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

A A-ZAP' cDNA library was made from Mangçfera indica L. cv. Tommy Atkins fruit that had been induced to ripen with ethene. The library was screened with a tomato ACC oxidase I (ACO) cDNA. A clone (pNY6OI) was isolated that appeared to encode the full amino acid sequence of ACO. Northern analysis showed that the corresponding message was expressed in untreated green fruit and fruit after 24 hours in ethylene hut declined in the later stages of ripening. A differential screen was then carried out to find ripening-specific clones. Two abundant types of clone were found as exemplified by the longest plasmid 'type clone" for each class: pNY6O8, and pNY642. In addition, four rare or types (pNY6O2, pNY627, pNY633 and pNY634) were found. None of these six clones showed any expression in green fruit hut all exhibited an increase in level after ethene treatment. When the cDNA inserts were sequenced and translated, pNY6O8, pNY627, pNY633 and pNY642 gave no convincing match to the SWISSPROT protein sequence database. However, pNY634 showed strong homology to a plastid chaperonin and pNY622 showed very strong homology to the Ypt3/Rabl 1 class of small GTPases. These have possible roles in extracellular and intracellular secretory pathways. It is also interesting to note that no clones related to known fruit ripening specific genes of tomato or other species were found during the differential screen.

Original languageEnglish
Title of host publicationActa Horticulturae
Pages277-286
Number of pages10
Volume455
Publication statusPublished - 1997
Externally publishedYes

Publication series

NameActa Horticulturae
Volume455
ISSN (Print)05677572

Fingerprint

mangoes
ripening
clones
fruits
aminocyclopropanecarboxylate oxidase
ethylene
amino acid sequences
tomatoes
chaperonins
guanosinetriphosphatase
cDNA libraries
plastids
plasmids
genes

ASJC Scopus subject areas

  • Horticulture

Cite this

Lycelt, G. W., Zainal, Z., Los, M., Findlay, C. L., & Tucker, G. A. (1997). Novel ripening-specific cdna clones from mango fruit. In Acta Horticulturae (Vol. 455, pp. 277-286). (Acta Horticulturae; Vol. 455).

Novel ripening-specific cdna clones from mango fruit. / Lycelt, Grantley W.; Zainal, Zamri; Los, Martin; Findlay, Caroline L.; Tucker, Gregory A.

Acta Horticulturae. Vol. 455 1997. p. 277-286 (Acta Horticulturae; Vol. 455).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lycelt, GW, Zainal, Z, Los, M, Findlay, CL & Tucker, GA 1997, Novel ripening-specific cdna clones from mango fruit. in Acta Horticulturae. vol. 455, Acta Horticulturae, vol. 455, pp. 277-286.
Lycelt GW, Zainal Z, Los M, Findlay CL, Tucker GA. Novel ripening-specific cdna clones from mango fruit. In Acta Horticulturae. Vol. 455. 1997. p. 277-286. (Acta Horticulturae).
Lycelt, Grantley W. ; Zainal, Zamri ; Los, Martin ; Findlay, Caroline L. ; Tucker, Gregory A. / Novel ripening-specific cdna clones from mango fruit. Acta Horticulturae. Vol. 455 1997. pp. 277-286 (Acta Horticulturae).
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