Novel peptides that inhibit the propagation of Newcastle disease virus

P. Ramanujam, W. S. Tan, Sheila Nathan, K. Yusoff

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A disulfide constrained random heptapeptide library displayed on filamentous bacteriophage M13 was applied to select specific ligands that interact with Newcastle disease virus (NDV). A fusion phage carrying the amino acid sequence TLTTKLY was selected from the panning procedure. An antibody competition assay showed that the selected phage was capable of competing with the polyclonal antibodies raised against NDV for binding sites on the virus. Determination of the binding affinity of this phage with NDV by an equilibrium binding assay in solution revealed two different dissociation constants, suggesting that there could be two distinct binding sites for the phage on NDV. Synthetic peptides with the sequence CTLTTKLYC, either in linear or cyclic conformations inhibited the binding of phage bearing the same sequence to NDV. These peptides also inhibited the hemolytic activity of the virus as well as its propagation in embryonated chicken eggs.

Original languageEnglish
Pages (from-to)981-993
Number of pages13
JournalArchives of Virology
Volume147
Issue number5
DOIs
Publication statusPublished - 2002

Fingerprint

Newcastle disease virus
Bacteriophages
Peptides
Virus Attachment
Inovirus
Binding Sites
Bacteriophage M13
Viruses
Antibodies
Disulfides
Eggs
Libraries
Amino Acid Sequence
Chickens
Ligands

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Novel peptides that inhibit the propagation of Newcastle disease virus. / Ramanujam, P.; Tan, W. S.; Nathan, Sheila; Yusoff, K.

In: Archives of Virology, Vol. 147, No. 5, 2002, p. 981-993.

Research output: Contribution to journalArticle

Ramanujam, P. ; Tan, W. S. ; Nathan, Sheila ; Yusoff, K. / Novel peptides that inhibit the propagation of Newcastle disease virus. In: Archives of Virology. 2002 ; Vol. 147, No. 5. pp. 981-993.
@article{65602fbbb7474b8b921b3c98da4a04e0,
title = "Novel peptides that inhibit the propagation of Newcastle disease virus",
abstract = "A disulfide constrained random heptapeptide library displayed on filamentous bacteriophage M13 was applied to select specific ligands that interact with Newcastle disease virus (NDV). A fusion phage carrying the amino acid sequence TLTTKLY was selected from the panning procedure. An antibody competition assay showed that the selected phage was capable of competing with the polyclonal antibodies raised against NDV for binding sites on the virus. Determination of the binding affinity of this phage with NDV by an equilibrium binding assay in solution revealed two different dissociation constants, suggesting that there could be two distinct binding sites for the phage on NDV. Synthetic peptides with the sequence CTLTTKLYC, either in linear or cyclic conformations inhibited the binding of phage bearing the same sequence to NDV. These peptides also inhibited the hemolytic activity of the virus as well as its propagation in embryonated chicken eggs.",
author = "P. Ramanujam and Tan, {W. S.} and Sheila Nathan and K. Yusoff",
year = "2002",
doi = "10.1007/s00705-001-0778-y",
language = "English",
volume = "147",
pages = "981--993",
journal = "Archives of Virology",
issn = "0304-8608",
publisher = "Springer Wien",
number = "5",

}

TY - JOUR

T1 - Novel peptides that inhibit the propagation of Newcastle disease virus

AU - Ramanujam, P.

AU - Tan, W. S.

AU - Nathan, Sheila

AU - Yusoff, K.

PY - 2002

Y1 - 2002

N2 - A disulfide constrained random heptapeptide library displayed on filamentous bacteriophage M13 was applied to select specific ligands that interact with Newcastle disease virus (NDV). A fusion phage carrying the amino acid sequence TLTTKLY was selected from the panning procedure. An antibody competition assay showed that the selected phage was capable of competing with the polyclonal antibodies raised against NDV for binding sites on the virus. Determination of the binding affinity of this phage with NDV by an equilibrium binding assay in solution revealed two different dissociation constants, suggesting that there could be two distinct binding sites for the phage on NDV. Synthetic peptides with the sequence CTLTTKLYC, either in linear or cyclic conformations inhibited the binding of phage bearing the same sequence to NDV. These peptides also inhibited the hemolytic activity of the virus as well as its propagation in embryonated chicken eggs.

AB - A disulfide constrained random heptapeptide library displayed on filamentous bacteriophage M13 was applied to select specific ligands that interact with Newcastle disease virus (NDV). A fusion phage carrying the amino acid sequence TLTTKLY was selected from the panning procedure. An antibody competition assay showed that the selected phage was capable of competing with the polyclonal antibodies raised against NDV for binding sites on the virus. Determination of the binding affinity of this phage with NDV by an equilibrium binding assay in solution revealed two different dissociation constants, suggesting that there could be two distinct binding sites for the phage on NDV. Synthetic peptides with the sequence CTLTTKLYC, either in linear or cyclic conformations inhibited the binding of phage bearing the same sequence to NDV. These peptides also inhibited the hemolytic activity of the virus as well as its propagation in embryonated chicken eggs.

UR - http://www.scopus.com/inward/record.url?scp=0036015507&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036015507&partnerID=8YFLogxK

U2 - 10.1007/s00705-001-0778-y

DO - 10.1007/s00705-001-0778-y

M3 - Article

VL - 147

SP - 981

EP - 993

JO - Archives of Virology

JF - Archives of Virology

SN - 0304-8608

IS - 5

ER -