Novel gold nanoparticles reduced by Sargassum glaucescens

Preparation, characterization and anticancer activity

Zahra Ajdari, Heshu Rahman, Kamyar Shameli, Rasedee Abdullah, Ma`Aruf Abd. Ghani, Swee Yeap, Sahar Abbasiliasi, Daniel Ajdari, Arbakariya Ariff

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The current study investigated the anticancer properties of gold nanoparticles (SG-stabilized AuNPs) synthesized using water extracts of the brown seaweed Sargassum glaucescens (SG). SG-stabilized AuNPs were characterized by ultraviolet-visible spectroscopy, transmission and scanning electron microscopy, and energy dispersive X-ray fluorescence spectrometry. The SG-stabilized AuNPs were stable and small at 3.65 ± 1.69 nm in size. The in vitro anticancer effect of SG-stabilized AuNPs was determined on cervical (HeLa), liver (HepG2), breast (MDA-MB-231) and leukemia (CEM-ss) cell lines using fluorescence microscopy, flow cytometry, caspase activity determination, and MTT assays. After 72 h treatment, SG-stabilized AuNPs was shown to be significant (p <0.05) cytotoxic to the cancer cells in a dose- and time-dependent manner. The IC50 values of SG-stabilized AuNPs on the HeLa, HepG2, CEM-ss, MDA-MB-231 cell lines were 4.75 ± 1.23, 7.14 ± 1.45, 10.32 ± 1.5, and 11.82 ± 0.9 μg/mL, respectively. On the other hand, SG-stabilized AuNPs showed no cytotoxic effect towards the normal human mammary epithelial cells (MCF-10A). SG-stabilized AuNPs significantly (p <0.05) arrest HeLa cell cycle at G2/M phase and significantly (p <0.05) activated caspases-3 and -9 activities. The anticancer effect of SG-stabilized AuNPs is via the intrinsic apoptotic pathway. The study showed that SG-stabilized AuNPs is a good candidate to be developed into a chemotherapeutic compound for the treatment of cancers especially cervical cancer.

Original languageEnglish
Article number123
JournalMolecules
Volume21
Issue number3
DOIs
Publication statusPublished - 1 Mar 2016

Fingerprint

Sargassum
Gold
Nanoparticles
multiple docking adapters
cancer
Cells
gold
cultured cells
nanoparticles
preparation
seaweeds
fluorescence
leukemias
cytometry
Seaweed
liver
breast
spectroscopy
Flow cytometry
Fluorescence microscopy

Keywords

  • Anticancer properties
  • Cervical cancer
  • Gold nanoparticles
  • In vitro study
  • Sargassum glaucescens

ASJC Scopus subject areas

  • Organic Chemistry

Cite this

Novel gold nanoparticles reduced by Sargassum glaucescens : Preparation, characterization and anticancer activity. / Ajdari, Zahra; Rahman, Heshu; Shameli, Kamyar; Abdullah, Rasedee; Abd. Ghani, Ma`Aruf; Yeap, Swee; Abbasiliasi, Sahar; Ajdari, Daniel; Ariff, Arbakariya.

In: Molecules, Vol. 21, No. 3, 123, 01.03.2016.

Research output: Contribution to journalArticle

Ajdari, Z, Rahman, H, Shameli, K, Abdullah, R, Abd. Ghani, MA, Yeap, S, Abbasiliasi, S, Ajdari, D & Ariff, A 2016, 'Novel gold nanoparticles reduced by Sargassum glaucescens: Preparation, characterization and anticancer activity', Molecules, vol. 21, no. 3, 123. https://doi.org/10.3390/molecules21030123
Ajdari, Zahra ; Rahman, Heshu ; Shameli, Kamyar ; Abdullah, Rasedee ; Abd. Ghani, Ma`Aruf ; Yeap, Swee ; Abbasiliasi, Sahar ; Ajdari, Daniel ; Ariff, Arbakariya. / Novel gold nanoparticles reduced by Sargassum glaucescens : Preparation, characterization and anticancer activity. In: Molecules. 2016 ; Vol. 21, No. 3.
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