Novel DNA Biosensor for Direct Determination of Carrageenan

Riyadh Abdulmalek Hassan, Yook Heng Lee, Ling Ling Tan @ Chong

Research output: Contribution to journalArticle

Abstract

A novel disposable electrochemical biosensor based on immobilized calf thymus double-stranded DNA (dsDNA) on the carbon-based screen-printed electrode (SPE) is developed for rapid biorecognition of carrageenan by using methylene blue (MB) redox indicator. The biosensor protocol for the detection of carrageenan is based on the concept of competitive binding of positively charged MB to the negatively charged dsDNA and carrageenan. The decrement in the MB cathodic peak current (i pc ) signal as a result of the released MB from the immobilized dsDNA, and attracted to the carrageenan can be monitored via differential pulse voltammetry (DPV). The biosensor showed high sensitivity and selectivity to carrageenan at low concentration without interference from other polyanions such as alginate, gum arabic and starch. Calibration of the biosensor with carrageenan exhibited an excellent linear dependence from 1–10 mg L −1 (R 2 = 0.98) with a detection limit of 0.08 mg L −1 . The DNA-based carrageenan biosensor showed satisfactory reproducibility with 5.6–6.9% (n = 3) relative standard deviations (RSD), and possessing several advantages such as simplicity, fast and direct application to real sample analysis without any prior extensive sample treatments, particularly for seaweeds and food analyses.

Original languageEnglish
Article number6379
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - 1 Dec 2019

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Carrageenan
Biosensing Techniques
Methylene Blue
Gum Arabic
Food Analysis
Seaweed
Competitive Binding
DNA
Starch
Thymus Gland
Calibration
Oxidation-Reduction
Limit of Detection
Electrodes
Carbon

ASJC Scopus subject areas

  • General

Cite this

Novel DNA Biosensor for Direct Determination of Carrageenan. / Hassan, Riyadh Abdulmalek; Lee, Yook Heng; Tan @ Chong, Ling Ling.

In: Scientific Reports, Vol. 9, No. 1, 6379, 01.12.2019.

Research output: Contribution to journalArticle

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