Non-viral transient transfection of hTERT gene into hBMSCs from elderly patients delays cellular aging in vitro

Min Hwei Ng, Aminuddin Bin Saim, Ruszymah Idrus

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Human bone marrow stem cell (hBMSC) is a promising candidate in regenerative medicine due to its plasticity and homing ability. However, in vitro survival and therapeutic efficacy of hBMSCs decline with donor age as a result of physiological cellular aging. Objectives: We transfected human telomerase reverse transcriptase (hTERT) into hBMSCs from patients more than 60 years old in the hope of extending cellular life span. Methods: In our study on 11 donors (aged 16-74 years), no telomerase activity was detected in hBMSCs from donors ≥60 years old. We then performed transient transfection on hBMSC from these elderly donors (n = 4) with hTERT gene using non-viral methods. Results: Transfection efficiency of 38.5% and 55.6% was achieved by lipofection and electroporation with viability at 83% and 44%, respectively. Telomerase was detected on day 5 for all post-transfected samples. Maximum passage number achieved after transfection was ≥13 (≈45 doublings) compared with 6 (≈15 doublings) before transfection. Conclusion: We conclude that transient expression of hTERT increased cellular life span of hBMSCs from elderly patients, while maintaining a normal cell morphology and growth rate.

Original languageEnglish
Pages (from-to)245-254
Number of pages10
JournalAsian Biomedicine
Volume6
Issue number2
DOIs
Publication statusPublished - Apr 2012

Fingerprint

Cell Aging
Transfection
Genes
Aging of materials
Telomerase
Tissue Donors
Stem cells
Bone
Bone Marrow Cells
Stem Cells
Regenerative Medicine
Electroporation
Plasticity
Survival
human TERT protein
In Vitro Techniques
Growth
Therapeutics

Keywords

  • Cellular life span
  • Human bone marrow stem cell
  • Telomerase
  • Transfection

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Non-viral transient transfection of hTERT gene into hBMSCs from elderly patients delays cellular aging in vitro. / Ng, Min Hwei; Saim, Aminuddin Bin; Idrus, Ruszymah.

In: Asian Biomedicine, Vol. 6, No. 2, 04.2012, p. 245-254.

Research output: Contribution to journalArticle

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