Nitric Oxide Sensing in Plants Is Mediated by Proteolytic Control of Group VII ERF Transcription Factors

Daniel J. Gibbs, Nurulhikma Md Isa, Mahsa Movahedi, Jorge Lozano-Juste, Guillermina M. Mendiondo, Sophie Berckhan, Nora Marín-delaRosa, Jorge VicenteConde, Cristina SousaCorreia, Simon P. Pearce, George W. Bassel, Bulut Hamali, Prabhavathi Talloji, Daniel F A Tomé, Alberto Coego, Jim Beynon, David Alabadí, Andreas Bachmair, José León, Julie E. GrayFrederica L. Theodoulou, Michael J. Holdsworth

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

Nitric oxide (NO) is an important signaling compound in prokaryotes and eukaryotes. In plants, NO regulates critical developmental transitions andstress responses. Here, we identify a mechanism for NO sensing that coordinates responses throughout development based on targeted degradation of plant-specific transcriptional regulators, the group VII ethylene response factors (ERFs). Weshow that the N-end rule pathway of targeted proteolysis targets these proteins for destruction in the presence of NO, and we establish them as critical regulators of diverse NO-regulated processes, including seed germination, stomatal closure, and hypocotyl elongation. Furthermore, we define the molecular mechanism for NO control of germinationand crosstalk with abscisic acid (ABA) signaling through ERF-regulated expression of ABSCISIC ACID INSENSITIVE5 (ABI5). Our work demonstrates how NO sensing is integrated across multiple physiological processes by direct modulation of transcription factor stability and identifies group VII ERFs as central hubs for the perception of gaseous signals in plants.

Original languageEnglish
Pages (from-to)369-379
Number of pages11
JournalMolecular Cell
Volume53
Issue number3
DOIs
Publication statusPublished - 6 Feb 2014

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Nitric Oxide
Transcription Factors
Control Groups
Abscisic Acid
Physiological Phenomena
Hypocotyl
Germination
ethylene
Eukaryota
Proteolysis
Seeds
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Gibbs, D. J., Md Isa, N., Movahedi, M., Lozano-Juste, J., Mendiondo, G. M., Berckhan, S., ... Holdsworth, M. J. (2014). Nitric Oxide Sensing in Plants Is Mediated by Proteolytic Control of Group VII ERF Transcription Factors. Molecular Cell, 53(3), 369-379. https://doi.org/10.1016/j.molcel.2013.12.020

Nitric Oxide Sensing in Plants Is Mediated by Proteolytic Control of Group VII ERF Transcription Factors. / Gibbs, Daniel J.; Md Isa, Nurulhikma; Movahedi, Mahsa; Lozano-Juste, Jorge; Mendiondo, Guillermina M.; Berckhan, Sophie; Marín-delaRosa, Nora; VicenteConde, Jorge; SousaCorreia, Cristina; Pearce, Simon P.; Bassel, George W.; Hamali, Bulut; Talloji, Prabhavathi; Tomé, Daniel F A; Coego, Alberto; Beynon, Jim; Alabadí, David; Bachmair, Andreas; León, José; Gray, Julie E.; Theodoulou, Frederica L.; Holdsworth, Michael J.

In: Molecular Cell, Vol. 53, No. 3, 06.02.2014, p. 369-379.

Research output: Contribution to journalArticle

Gibbs, DJ, Md Isa, N, Movahedi, M, Lozano-Juste, J, Mendiondo, GM, Berckhan, S, Marín-delaRosa, N, VicenteConde, J, SousaCorreia, C, Pearce, SP, Bassel, GW, Hamali, B, Talloji, P, Tomé, DFA, Coego, A, Beynon, J, Alabadí, D, Bachmair, A, León, J, Gray, JE, Theodoulou, FL & Holdsworth, MJ 2014, 'Nitric Oxide Sensing in Plants Is Mediated by Proteolytic Control of Group VII ERF Transcription Factors', Molecular Cell, vol. 53, no. 3, pp. 369-379. https://doi.org/10.1016/j.molcel.2013.12.020
Gibbs, Daniel J. ; Md Isa, Nurulhikma ; Movahedi, Mahsa ; Lozano-Juste, Jorge ; Mendiondo, Guillermina M. ; Berckhan, Sophie ; Marín-delaRosa, Nora ; VicenteConde, Jorge ; SousaCorreia, Cristina ; Pearce, Simon P. ; Bassel, George W. ; Hamali, Bulut ; Talloji, Prabhavathi ; Tomé, Daniel F A ; Coego, Alberto ; Beynon, Jim ; Alabadí, David ; Bachmair, Andreas ; León, José ; Gray, Julie E. ; Theodoulou, Frederica L. ; Holdsworth, Michael J. / Nitric Oxide Sensing in Plants Is Mediated by Proteolytic Control of Group VII ERF Transcription Factors. In: Molecular Cell. 2014 ; Vol. 53, No. 3. pp. 369-379.
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