Nile Blue chromoionophore-doped kappa-carrageenan for a novel reflectometric urea biosensor

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Abstract A new optical urea biosensor based on urease (Urs) physically attached to a pH optode made from Nile Blue (NB) chromoionophore-doped kappa-carrageenan (KC) membrane is described in this paper. The immobilized Urs on the biosensor surface catalyzed the hydrolytic decomposition of urea into ammonium hydroxide, a pH changed to a more basic condition, and resulted in a colour change of the pH sensor membrane from blue to violet, which can be quantified reflectometrically using a fibre optic reflectance spectrophotometer. A linear relationship was perceived between the reflectance intensity of the KC membrane-based biosensor and the urea concentration in the range of 0.001-100 mM with the detection limit calculated at 0.001 mM. The response time of the urea biosensor was 10 min, and no significant discrepancy was noted for the results obtained from the direct analysis of urine samples by both the reflectance catalytic biosensor and the UV-vis spectrophotometric standard method based on the p-dimethylaminobenzaldehyde (DMAB) reagent.

Original languageEnglish
Article number18768
Pages (from-to)969-977
Number of pages9
JournalSensors and Actuators, B: Chemical
Volume221
DOIs
Publication statusPublished - 3 Aug 2015

Fingerprint

Carrageenan
ureas
bioinstrumentation
Biosensors
Urea
Urease
membranes
Membranes
reflectance
Ammonium Hydroxide
pH sensors
Ammonium hydroxide
urine
Spectrophotometers
spectrophotometers
Fiber optics
hydroxides
reagents
fiber optics
Nile Blue

Keywords

  • Kappa-carrageenan
  • Nile Blue chromoionophore
  • Optical urea biosensor
  • Reflectometric
  • Urease

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials
  • Metals and Alloys
  • Surfaces, Coatings and Films
  • Materials Chemistry
  • Instrumentation

Cite this

Nile Blue chromoionophore-doped kappa-carrageenan for a novel reflectometric urea biosensor. / Esmaeili, Chakavak; Lee, Yook Heng; Tan @ Chong, Ling Ling.

In: Sensors and Actuators, B: Chemical, Vol. 221, 18768, 03.08.2015, p. 969-977.

Research output: Contribution to journalArticle

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