New scenario for speciation in the benthic dinoflagellate genus Coolia (Dinophyceae)

Chui Pin Leaw, Toh Hii Tan, Hong Chang Lim, Sing Tung Teng, Hwa Lin Yong, Kirsty F. Smith, Lesley Rhodes, Matthias Wolf, William C. Holland, Mark W. Vandersea, R. Wayne Litaker, Patricia A. Tester, Haifeng Gu, Gires Usup, Po Teen Lim

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In this study, inter- and intraspecific genetic diversity within the marine harmful dinoflagellate genus Coolia Meunier was evaluated using isolates obtained from the tropics to subtropics in both Pacific and Atlantic Ocean basins. The aim was to assess the phylogeographic history of the genus and to clarify the validity of established species including Coolia malayensis. Phylogenetic analysis of the D1-D2 LSU rDNA sequences identified six major lineages (L1-L6) corresponding to the morphospecies Coolia malayensis (L1), C. monotis (L2), C. santacroce (L3), C. palmyrensis (L4), C. tropicalis (L5), and C. canariensis (L6). A median joining network (MJN) of C. malayensis ITS2 rDNA sequences revealed a total of 16 haplotypes; however, no spatial genetic differentiation among populations was observed. These MJN results in conjunction with CBC analysis, rDNA phylogenies and geographical distribution analyses confirm C. malayensis as a distinct species which is globally distributed in the tropical to warm-temperate regions. A molecular clock analysis using ITS2 rDNA revealed the evolutionary history of Coolia dated back to the Mesozoic, and supports the hypothesis that historical vicariant events in the early Cenozoic drove the allopatric differentiation of C. malayensis and C. monotis.

Original languageEnglish
Pages (from-to)137-149
Number of pages13
JournalHarmful Algae
Volume55
DOIs
Publication statusPublished - 1 May 2016

Fingerprint

dinoflagellate
Dinophyceae
genetic variation
history
molecular analysis
phylogeny
ocean basin
subtropics
Atlantic Ocean
genetic differentiation
geographical distribution
Pacific Ocean
tropics
haplotypes
basins
phylogenetics
Miozoa
analysis
morphospecies
genetic diversity

Keywords

  • Benthic harmful algal bloom (BHAB)
  • Coolia malayensis
  • Coolia monotis
  • Coolia tropicalis
  • Phylogeography
  • Second internal transcribed spacer (ITS2)

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Cite this

Leaw, C. P., Tan, T. H., Lim, H. C., Teng, S. T., Yong, H. L., Smith, K. F., ... Lim, P. T. (2016). New scenario for speciation in the benthic dinoflagellate genus Coolia (Dinophyceae). Harmful Algae, 55, 137-149. https://doi.org/10.1016/j.hal.2016.02.010

New scenario for speciation in the benthic dinoflagellate genus Coolia (Dinophyceae). / Leaw, Chui Pin; Tan, Toh Hii; Lim, Hong Chang; Teng, Sing Tung; Yong, Hwa Lin; Smith, Kirsty F.; Rhodes, Lesley; Wolf, Matthias; Holland, William C.; Vandersea, Mark W.; Litaker, R. Wayne; Tester, Patricia A.; Gu, Haifeng; Usup, Gires; Lim, Po Teen.

In: Harmful Algae, Vol. 55, 01.05.2016, p. 137-149.

Research output: Contribution to journalArticle

Leaw, CP, Tan, TH, Lim, HC, Teng, ST, Yong, HL, Smith, KF, Rhodes, L, Wolf, M, Holland, WC, Vandersea, MW, Litaker, RW, Tester, PA, Gu, H, Usup, G & Lim, PT 2016, 'New scenario for speciation in the benthic dinoflagellate genus Coolia (Dinophyceae)', Harmful Algae, vol. 55, pp. 137-149. https://doi.org/10.1016/j.hal.2016.02.010
Leaw, Chui Pin ; Tan, Toh Hii ; Lim, Hong Chang ; Teng, Sing Tung ; Yong, Hwa Lin ; Smith, Kirsty F. ; Rhodes, Lesley ; Wolf, Matthias ; Holland, William C. ; Vandersea, Mark W. ; Litaker, R. Wayne ; Tester, Patricia A. ; Gu, Haifeng ; Usup, Gires ; Lim, Po Teen. / New scenario for speciation in the benthic dinoflagellate genus Coolia (Dinophyceae). In: Harmful Algae. 2016 ; Vol. 55. pp. 137-149.
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