New immunomodulatory steroidal alkaloids from Sarcococa saligna

Naveed Iqbal, Achyut Adhikari, Nayab Kanwal, Omer Mohamed Abdalla, Osman Mesaik Mohammed Ahmed Hassan, Syed Ghulam Musharraf

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Two new steroidal alkaloids, (20 S)-(bennzamido)-3β-(N,N-dimethyamino)-pregnane (1), and (20 S)-(bennzamido)-pregnane-3-one- (2), and two known steroidal alkaloids, pachysanaximine A (3) and 3β, 20α-diacetamido-5α-pregnane (4) were isolated from the whole plant of Sarcococca saligna. The structures of these compounds were identified with the help of spectroscopic techniques while spectra for known compounds were compared with spectra reported in literature. The immunomodulatory potential of the new compounds were found to be significant and dose dependent. Compound 1 showed inhibition of T cells proliferation at 10 μg/mL (95%), and inhibition of IL-2 production with an IC50 = 1.6 μg/mL.

Original languageEnglish
Pages (from-to)203-208
Number of pages6
JournalPhytochemistry Letters
Volume14
DOIs
Publication statusPublished - 1 Dec 2015

Fingerprint

Pregnanes
Alkaloids
Sarcococca saligna
alkaloids
Buxaceae
interleukin-2
inhibitory concentration 50
cell proliferation
T-cells
T-lymphocytes
Cell proliferation
Inhibitory Concentration 50
Interleukin-2
dosage
Cell Proliferation
T-Lymphocytes
methodology

Keywords

  • Immunomodulatory activity
  • Sarcococca saligna
  • Steroidal alkaloids

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science
  • Biochemistry
  • Biotechnology

Cite this

Iqbal, N., Adhikari, A., Kanwal, N., Abdalla, O. M., Mohammed Ahmed Hassan, O. M., & Musharraf, S. G. (2015). New immunomodulatory steroidal alkaloids from Sarcococa saligna. Phytochemistry Letters, 14, 203-208. https://doi.org/10.1016/j.phytol.2015.10.009

New immunomodulatory steroidal alkaloids from Sarcococa saligna. / Iqbal, Naveed; Adhikari, Achyut; Kanwal, Nayab; Abdalla, Omer Mohamed; Mohammed Ahmed Hassan, Osman Mesaik; Musharraf, Syed Ghulam.

In: Phytochemistry Letters, Vol. 14, 01.12.2015, p. 203-208.

Research output: Contribution to journalArticle

Iqbal, N, Adhikari, A, Kanwal, N, Abdalla, OM, Mohammed Ahmed Hassan, OM & Musharraf, SG 2015, 'New immunomodulatory steroidal alkaloids from Sarcococa saligna', Phytochemistry Letters, vol. 14, pp. 203-208. https://doi.org/10.1016/j.phytol.2015.10.009
Iqbal N, Adhikari A, Kanwal N, Abdalla OM, Mohammed Ahmed Hassan OM, Musharraf SG. New immunomodulatory steroidal alkaloids from Sarcococa saligna. Phytochemistry Letters. 2015 Dec 1;14:203-208. https://doi.org/10.1016/j.phytol.2015.10.009
Iqbal, Naveed ; Adhikari, Achyut ; Kanwal, Nayab ; Abdalla, Omer Mohamed ; Mohammed Ahmed Hassan, Osman Mesaik ; Musharraf, Syed Ghulam. / New immunomodulatory steroidal alkaloids from Sarcococa saligna. In: Phytochemistry Letters. 2015 ; Vol. 14. pp. 203-208.
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