Neutralization of Burkholderia pseudomallei protease by Fabs generated through phage display

Sheila Nathan, Christoph Rader, Carlos F. Barbas

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The isolation of therapeutic and functional protease inhibitors in vitro via combinatorial chemistry and phage display technology has been described previously. Here we report the construction of a combinatorial mouse-human chimeric antibody fragment (Fab) antibody library targeted against the protease of the tropical pathogen, Burkholderia pseudomallei. The resulting library was biopanned against the protease, and selected clones were analyzed for their ability to function as protease inhibitors. Three families of Fabs were identified by restriction fingerprinting, all of which demonstrated high specificity towards the protease of B. pseudomallei. Purified Fabs also demonstrated the capacity to inhibit B. pseudomallei protease activity in vitro, and this inhibitory property was exclusive to the pathogenic protease. Thus these recombinant antibodies are candidates for immunotherapy and tools to aid in further elucidation of the mechanism of action of the B. pseudomallei protease.

Original languageEnglish
Pages (from-to)2302-2311
Number of pages10
JournalBioscience, Biotechnology and Biochemistry
Volume69
Issue number12
DOIs
Publication statusPublished - 2005

Fingerprint

Burkholderia pseudomallei
Bacteriophages
neutralization
bacteriophages
Peptide Hydrolases
proteinases
Display devices
Antibodies
proteinase inhibitors
Protease Inhibitors
recombinant antibodies
Immunoglobulin Fragments
antibodies
immunotherapy
Pathogens
Immunotherapy
Libraries
mechanism of action
chemistry
Clone Cells

Keywords

  • Burkholderia pseudomallei
  • Fab
  • Phage display
  • Protease

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science

Cite this

Neutralization of Burkholderia pseudomallei protease by Fabs generated through phage display. / Nathan, Sheila; Rader, Christoph; Barbas, Carlos F.

In: Bioscience, Biotechnology and Biochemistry, Vol. 69, No. 12, 2005, p. 2302-2311.

Research output: Contribution to journalArticle

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