Neuropeptide coding of sympathetic preganglionic neurons; focus on adrenally projecting populations

N. N. Kumar, K. Allen, L. Parker, Mohd Hanafi Ahmad Damanhuri, A. K. Goodchild

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Chemical coding of sympathetic preganglionic neurons (SPN) suggests that the chemical content of subpopulations of SPN can define their function. Since neuropeptides, once synthesized are transported to the axon terminal, most demonstrated chemical coding has been identified using immunoreactive terminals at the target organ. Here, we use a different approach to identify and quantify the subpopulations of SPN that contain the mRNA for pituitary adenylate cyclase activating polypeptide (PACAP) or enkephalin. Using double-labeled immunohistochemistry combined with in situ hybridization (ISH) we firstly identified the distribution of these mRNAs in the spinal cord and determined quantitatively, in Sprague-Dawley rats, that many SPN at the T4-T10 spinal level contain preproPACAP (PPP+, 80±3%, n=3), whereas a very small percentage contain preproenkephalin (PPE+, 4±2%, n=4). A similar neurochemical distribution was found at C8-T3 spinal level. These data suggest that PACAP potentially regulates a large number of functions dictated by SPN whereas enkephalins are involved in few functions. We extended the study to explore those SPN that control adrenal chromaffin cells. We found 97±5% of adrenally projecting SPN (AP-SPN) to be PPP+ (n=4) with only 47±3% that were PPE+ (n=5). These data indicate that adrenally projecting PACAPergic SPN regulate both adrenal adrenaline (Ad) and noradrenaline (NAd) release whereas the enkephalinergic SPN subpopulation must control a (sub) population of chromaffin cells - most likely those that release Ad. The sensory innervation of the adrenal gland was also determined. Of the few adrenally projecting dorsal root ganglia (AP-DRG) observed, 74±12% were PPP+ (n=3), whereas 1±1% were PPE+ (n=3). Therefore, if sensory neurons release peptides to the adrenal medulla, PACAP is most likely involved. Together, these data provide a neurochemical basis for differential control of sympathetic outflow particularly that to the adrenal medulla.

Original languageEnglish
Pages (from-to)789-799
Number of pages11
JournalNeuroscience
Volume170
Issue number3
DOIs
Publication statusPublished - Oct 2010
Externally publishedYes

Fingerprint

Neuropeptides
Neurons
Population
Pituitary Adenylate Cyclase-Activating Polypeptide
Chromaffin Cells
Adrenal Medulla
Enkephalins
Epinephrine
Messenger RNA
Presynaptic Terminals
Spinal Ganglia
Sensory Receptor Cells
Adrenal Glands
In Situ Hybridization
Sprague Dawley Rats
Spinal Cord
Norepinephrine
Immunohistochemistry
Peptides

Keywords

  • Adrenaline
  • Dorsal root ganglia
  • Enkephalin
  • Noradrenaline
  • PACAP

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Neuropeptide coding of sympathetic preganglionic neurons; focus on adrenally projecting populations. / Kumar, N. N.; Allen, K.; Parker, L.; Ahmad Damanhuri, Mohd Hanafi; Goodchild, A. K.

In: Neuroscience, Vol. 170, No. 3, 10.2010, p. 789-799.

Research output: Contribution to journalArticle

Kumar, N. N. ; Allen, K. ; Parker, L. ; Ahmad Damanhuri, Mohd Hanafi ; Goodchild, A. K. / Neuropeptide coding of sympathetic preganglionic neurons; focus on adrenally projecting populations. In: Neuroscience. 2010 ; Vol. 170, No. 3. pp. 789-799.
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