Nested PCR for the rapid detection of TB from pleural fluid at HUKM Malaysia

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5 Citations (Scopus)

Abstract

The aim of the present study is rapid detection of tuberculosis from pleural effusion of suspected patients. Molecular technique Nested Polymerase Chain Reaction (PCR) was used for the purpose. A total of 67 pleural fluid collected at Hospital University Kebangsaan Malaysia during May 2005 to October 2006 were sent to Microbiology Laboratory enrolled in the study. Detection rate of Mycobacterium tuberculosis in pleural effusion was 0% by acid-fast bacilli (AFB) staining and 1.5% by culture on Lowenstein-Jensen medium. Mycobacterium tuberculosis was detected by PCR in 9% of the cases. PCR of pleural fluid had 19% sensitivity and 96% specificity, compared to AFB staining (0% sensitivity and 100% specificity) and culture (4% sensitivity and 100% specificity). PCR also has 67% Positive Predictive Value (PPV) and 72% Negative Predictive Value (NPV) in detecting Mycobacterium tuberculosis. Culture of pleural fluid has 100% PPV and 71% NPV while AFB staining has 0% PPV and 31% NPV. This preliminary study showed that PCR is a rapid method for detection of Mycobacterium tuberculosis in pleural fluid but its sensitivity is not up the marked.

Original languageEnglish
Pages (from-to)1728-1732
Number of pages5
JournalPakistan Journal of Biological Sciences
Volume11
Issue number13
DOIs
Publication statusPublished - 2008

Fingerprint

Malaysia
Mycobacterium tuberculosis
polymerase chain reaction
acids
acid value
microbiology
tuberculosis
rapid methods
fluids
staining
Bacilli
methodology

Keywords

  • Acid fast stain
  • Mycobacterium tuberculosis
  • Nested PCR
  • Pleural fluid

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

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title = "Nested PCR for the rapid detection of TB from pleural fluid at HUKM Malaysia",
abstract = "The aim of the present study is rapid detection of tuberculosis from pleural effusion of suspected patients. Molecular technique Nested Polymerase Chain Reaction (PCR) was used for the purpose. A total of 67 pleural fluid collected at Hospital University Kebangsaan Malaysia during May 2005 to October 2006 were sent to Microbiology Laboratory enrolled in the study. Detection rate of Mycobacterium tuberculosis in pleural effusion was 0{\%} by acid-fast bacilli (AFB) staining and 1.5{\%} by culture on Lowenstein-Jensen medium. Mycobacterium tuberculosis was detected by PCR in 9{\%} of the cases. PCR of pleural fluid had 19{\%} sensitivity and 96{\%} specificity, compared to AFB staining (0{\%} sensitivity and 100{\%} specificity) and culture (4{\%} sensitivity and 100{\%} specificity). PCR also has 67{\%} Positive Predictive Value (PPV) and 72{\%} Negative Predictive Value (NPV) in detecting Mycobacterium tuberculosis. Culture of pleural fluid has 100{\%} PPV and 71{\%} NPV while AFB staining has 0{\%} PPV and 31{\%} NPV. This preliminary study showed that PCR is a rapid method for detection of Mycobacterium tuberculosis in pleural fluid but its sensitivity is not up the marked.",
keywords = "Acid fast stain, Mycobacterium tuberculosis, Nested PCR, Pleural fluid",
author = "Salleh, {Sharifah Azura} and Salasawati Hussin and Rahman, {Md. Mostafizur}",
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AU - Hussin, Salasawati

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N2 - The aim of the present study is rapid detection of tuberculosis from pleural effusion of suspected patients. Molecular technique Nested Polymerase Chain Reaction (PCR) was used for the purpose. A total of 67 pleural fluid collected at Hospital University Kebangsaan Malaysia during May 2005 to October 2006 were sent to Microbiology Laboratory enrolled in the study. Detection rate of Mycobacterium tuberculosis in pleural effusion was 0% by acid-fast bacilli (AFB) staining and 1.5% by culture on Lowenstein-Jensen medium. Mycobacterium tuberculosis was detected by PCR in 9% of the cases. PCR of pleural fluid had 19% sensitivity and 96% specificity, compared to AFB staining (0% sensitivity and 100% specificity) and culture (4% sensitivity and 100% specificity). PCR also has 67% Positive Predictive Value (PPV) and 72% Negative Predictive Value (NPV) in detecting Mycobacterium tuberculosis. Culture of pleural fluid has 100% PPV and 71% NPV while AFB staining has 0% PPV and 31% NPV. This preliminary study showed that PCR is a rapid method for detection of Mycobacterium tuberculosis in pleural fluid but its sensitivity is not up the marked.

AB - The aim of the present study is rapid detection of tuberculosis from pleural effusion of suspected patients. Molecular technique Nested Polymerase Chain Reaction (PCR) was used for the purpose. A total of 67 pleural fluid collected at Hospital University Kebangsaan Malaysia during May 2005 to October 2006 were sent to Microbiology Laboratory enrolled in the study. Detection rate of Mycobacterium tuberculosis in pleural effusion was 0% by acid-fast bacilli (AFB) staining and 1.5% by culture on Lowenstein-Jensen medium. Mycobacterium tuberculosis was detected by PCR in 9% of the cases. PCR of pleural fluid had 19% sensitivity and 96% specificity, compared to AFB staining (0% sensitivity and 100% specificity) and culture (4% sensitivity and 100% specificity). PCR also has 67% Positive Predictive Value (PPV) and 72% Negative Predictive Value (NPV) in detecting Mycobacterium tuberculosis. Culture of pleural fluid has 100% PPV and 71% NPV while AFB staining has 0% PPV and 31% NPV. This preliminary study showed that PCR is a rapid method for detection of Mycobacterium tuberculosis in pleural fluid but its sensitivity is not up the marked.

KW - Acid fast stain

KW - Mycobacterium tuberculosis

KW - Nested PCR

KW - Pleural fluid

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