Nanoscale diblock copolymer micelles

Characterizations and estimation of the effective diffusion coefficients of biomolecules release through cylindrical diffusion model

M. Wahab Amjad, Mohd Cairul Iqbal Mohd Amin, Shalela M. Mahali, Haliza Katas, Ismanizan Ismail, M. Naeem Ul Hassan, Victor T. Giam Chuang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Biomolecules have been widely investigated as potential therapeutics for various diseases. However their use is limited due to rapid degradation and poor cellular uptake in vitro and in vivo. To address this issue, we synthesized a new nano-carrier system comprising of cholic acid-polyethylenimine (CA-PEI) copolymer micelles, via carbodiimide-mediated coupling for the efficient delivery of small interfering ribonucleic acid (siRNA) and bovine serum albumin (BSA) as model protein. The mean particle size of siRNA- or BSA-loaded CA-PEI micelles ranged from 100-150 nm, with zeta potentials of +3-+11 mV, respectively. Atomic force, transmission electron and field emission scanning electron microscopy demonstrated that the micelles exhibited excellent spherical morphology. No significant morphology or size changes were observed in the CA-PEI micelles after siRNA and BSA loading. CA-PEI micelles exhibited sustained release profile, the effective diffusion coefficients were successfully estimated using a mathematically-derived cylindrical diffusion model and the release data of siRNA and BSA closely fitted into this model. High siRNA and BSA binding and loading efficiencies (95% and 70%, respectively) were observed for CA-PEI micelles. Stability studies demonstrated that siRNA and BSA integrity was maintained after loading and release. The CA-PEI micelles were non cytotoxic to V79 and DLD-1 cells, as shown by alamarBlue and LIVE/DEAD cell viability assays. RT-PCR study revealed that siRNA-loaded CA-PEI micelles suppressed the mRNA for ABCB1 gene. These results revealed the promising potential of CA-PEI micelles as a stable, safe, and versatile nano-carrier for siRNA and the model protein delivery.

Original languageEnglish
Article numbere105234
JournalPLoS One
Volume9
Issue number8
DOIs
Publication statusPublished - 18 Aug 2014

Fingerprint

cholic acid
composite polymers
Cholic Acid
Polyethyleneimine
Micelles
Biomolecules
micelles
diffusivity
Block copolymers
bovine serum albumin
Bovine Serum Albumin
RNA
Carbodiimides
Electron emission
Zeta potential
Particle Size
Field emission
Electron Scanning Microscopy
cell viability
particle size

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Nanoscale diblock copolymer micelles : Characterizations and estimation of the effective diffusion coefficients of biomolecules release through cylindrical diffusion model. / Amjad, M. Wahab; Mohd Amin, Mohd Cairul Iqbal; Mahali, Shalela M.; Katas, Haliza; Ismail, Ismanizan; Naeem Ul Hassan, M.; Giam Chuang, Victor T.

In: PLoS One, Vol. 9, No. 8, e105234, 18.08.2014.

Research output: Contribution to journalArticle

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