Nanodiagnostics performed on human red blood cells with atomic force microscopy

O. Hekele, C. G. Goesselsberger, I. C. Gebeshuber

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Atomic force microscopy can yield valuable information concerning basic physical properties as well as alterations of human red blood cells. Erythropoietin is a hormone that is naturally produced in the kidney to stimulate the growth of red blood cells. Administration of genetically engineered synthetic erythropoietin stimulates the production of additional red blood cells. Therefore erythropoietin is used for blood doping in serious sports. The present study aims at investigating any differences in structure and stiffness of red blood cells which are produced body own or with synthetic erythropoietin. The samples are prepared via standard methods, and atomic force spectroscopy with trigger forces of three micronewtons is performed in ambient air. The penetration depth does not reveal statistically relevant differences in the two types of red blood cells. Furthermore, cells with a penetration depth four times as large as healthy ones are encountered in the samples of one donor. Subsequent medical examination revealed a rare type of diabetes. Atomic force spectroscopy shall serve as fast screening method for nanodiagnostics of diseases that alter surface nanomechanical properties.

Original languageEnglish
Pages (from-to)1162-1165
Number of pages4
JournalMaterials Science and Technology
Volume24
Issue number9
DOIs
Publication statusPublished - Sep 2008
Externally publishedYes

Fingerprint

erythrocytes
Atomic force microscopy
Blood
Cells
atomic force microscopy
Erythropoietin
penetration
Spectroscopy
hormones
kidneys
surface properties
Hormones
spectroscopy
blood
Medical problems
Sports
stiffness
screening
physical properties
examination

Keywords

  • Atomic force microscope
  • EPO
  • Erythrocytes
  • Erythropoietin
  • Force mapping
  • Red blood cells

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials

Cite this

Nanodiagnostics performed on human red blood cells with atomic force microscopy. / Hekele, O.; Goesselsberger, C. G.; Gebeshuber, I. C.

In: Materials Science and Technology, Vol. 24, No. 9, 09.2008, p. 1162-1165.

Research output: Contribution to journalArticle

Hekele, O. ; Goesselsberger, C. G. ; Gebeshuber, I. C. / Nanodiagnostics performed on human red blood cells with atomic force microscopy. In: Materials Science and Technology. 2008 ; Vol. 24, No. 9. pp. 1162-1165.
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