Mutagenicity effect of Hydrocotyle bonariensis extracts in Salmonella/microsome assay

L. Florinsiah, Zuraina Y M Farida, R. Norfadilah, N. Nurshahirah, N. M. Norfazlina, Zaila C F Suzaina

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Several medicinal plants are traditionally used in the treatment of a variety of ailments including cancer. However, many of these herbs have not been scientifically assessed for its efficacy or safety. The mutagenic effect of aqueous and methanolic extracts obtained from aerial parts (leaves and stem) and roots of Hydrocotyle bonariensis were assayed using the Salmonella typhimurium assay or Ames test. The pre-incubation method was used on S. typhimurium TA 98 and TA 100 bacterial strains in the presence and absence of metabolic activator S9 system. The results showed that all aqueous extracts of the aerial parts and roots of H.bonariensis were not mutagenic towards TA98 and TA100 strain with and without S9 activation using all concentration. On the other hand, the methanolic extracts of leaves, stems and roots were also not mutagenic in the absence of metabolic activation against both strains. However, with metabolic activation, only methanolic extracts of H.bonariensis leaves and stems showed mutagenic effect at the highest concentration (50mg/ml) towards TA98 strain but both were not mutagenic for TA100 in all concentration studied. In conclusion, H.bonariensis extracts were not mutagenic on both S. typhimurium strains and has the potential to be used as part of traditional medicine.

Original languageEnglish
Pages (from-to)47-50
Number of pages4
JournalInternational Journal of Pharmaceutical Sciences Review and Research
Volume20
Issue number2
Publication statusPublished - Jun 2013

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Centella
Salmonella typhimurium
Microsomes
Salmonella
Traditional Medicine
Medicinal Plants
Safety
Neoplasms
Metabolic Activation
Therapeutics

Keywords

  • Ames assay
  • Hydrocotyle bonariensis
  • Mutagenicity effect
  • Salmonella typhimurium

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Florinsiah, L., Farida, Z. Y. M., Norfadilah, R., Nurshahirah, N., Norfazlina, N. M., & Suzaina, Z. C. F. (2013). Mutagenicity effect of Hydrocotyle bonariensis extracts in Salmonella/microsome assay. International Journal of Pharmaceutical Sciences Review and Research, 20(2), 47-50.

Mutagenicity effect of Hydrocotyle bonariensis extracts in Salmonella/microsome assay. / Florinsiah, L.; Farida, Zuraina Y M; Norfadilah, R.; Nurshahirah, N.; Norfazlina, N. M.; Suzaina, Zaila C F.

In: International Journal of Pharmaceutical Sciences Review and Research, Vol. 20, No. 2, 06.2013, p. 47-50.

Research output: Contribution to journalArticle

Florinsiah, L, Farida, ZYM, Norfadilah, R, Nurshahirah, N, Norfazlina, NM & Suzaina, ZCF 2013, 'Mutagenicity effect of Hydrocotyle bonariensis extracts in Salmonella/microsome assay', International Journal of Pharmaceutical Sciences Review and Research, vol. 20, no. 2, pp. 47-50.
Florinsiah, L. ; Farida, Zuraina Y M ; Norfadilah, R. ; Nurshahirah, N. ; Norfazlina, N. M. ; Suzaina, Zaila C F. / Mutagenicity effect of Hydrocotyle bonariensis extracts in Salmonella/microsome assay. In: International Journal of Pharmaceutical Sciences Review and Research. 2013 ; Vol. 20, No. 2. pp. 47-50.
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