Mutagenic and clastogenic characterization of poststerilized poly(3-hydroxybutyrate-co-4-hydroxybutyrate) copolymer biosynthesized by Delftia acidovorans

Ling Siew Ee, Nor Fadilah Rajab, Annuar Bin Osman, Kumar Sudesh, Salmaan Hussain Inayat-Hussain

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Polyhydroxyalkanoates (PHA) are naturally occurring biopolyesters that have great potential in the medical field. However, the leachables resulting from sterilization process of the biomaterials may exert toxic effect including genetic damage. Here, we demonstrate that although gamma-irradiation of poly(3-hydroxybutyrate-co-50 mol % 4-hydroxybutyrate) [P(3HB-co-4HB)] did not cause any change in the morphology by scanning electron microscopy, there was a significant degradation of this copolymer where the molecular weight was reduced by 37% after sterilization indicating the generation of leachables. Therefore, further investigation on the ability of the extract of this poststerilized copolymer to induce mutagenic effect was performed using Ames test (S. typhimurium strains TA1535 and TA1537) and umu test (S. typhimurium strain TA1535/pSK1002). Additionally, the capability of the extract to induce clastogenic effect was determined using Chinese hamster lung V79 fibroblast cells. Our results showed that with and without the presence of S9 metabolic activation, no mutagenic effects were observed in both Ames and umu tests when treated with P(3HB-co-4HB) extract. Similarly, treatment of P(3HB-co-4HB) extract in V79 fibroblast cells showed no significant production of micronuclei when compared with the positive control (Mitomycin C). Together, these results indicate that leachables of poststerilized P(3HB-co-4HB) cause no mutagenic and clastogenic effects.

Original languageEnglish
Pages (from-to)786-794
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume91
Issue number3
DOIs
Publication statusPublished - Dec 2009

Fingerprint

Hydroxybutyrates
Fibroblasts
3-Hydroxybutyric Acid
Copolymers
Cells
Biomaterials
Chemical activation
Molecular weight
Irradiation
Polyhydroxyalkanoates
Degradation
Scanning electron microscopy
Poisons
Biocompatible Materials
Mitomycin
poly(3-hydroxybutyrate-co-4-hydroxybutyrate)

Keywords

  • Ames test
  • Fibroblast
  • Genotoxicity
  • Micronucleus
  • Polyhydroxyalkanoates
  • Umu test

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys

Cite this

Mutagenic and clastogenic characterization of poststerilized poly(3-hydroxybutyrate-co-4-hydroxybutyrate) copolymer biosynthesized by Delftia acidovorans. / Ee, Ling Siew; Rajab, Nor Fadilah; Osman, Annuar Bin; Sudesh, Kumar; Inayat-Hussain, Salmaan Hussain.

In: Journal of Biomedical Materials Research - Part A, Vol. 91, No. 3, 12.2009, p. 786-794.

Research output: Contribution to journalArticle

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