Mumia flava gen. nov., sp. nov., an actinobacterium of the family Nocardioidaceae

Learn Han Lee, Nurullhudda Zainal, Adzzie Shazleen Azman, Nurul Syakima Ab Mutalib, Kui Hong, Kok Gan Chan

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A novel actinobacterial strain, designated MUSC 201T, was isolated from a mangrove soil collected from Kuantan, the capital city of Pahang State in Malaysia. The taxonomic status of this strain was determined using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain MUSC 201T represented a novel lineage within the class Actinobacteria. Strain MUSC 201T formed a distinct clade in the family Nocardioidaceae and was most closely related to the members of the genera Nocardioides (16S rRNA gene sequence similarity, 91.9-95.1 %), Aeromicrobium (92.7-94.6 %), Marmoricola (92.5-93.1 %) and Kribbella (91.5-92.4 %). The cells of this strain were irregular coccoid to short rod shaped. The peptidoglycan contained LL-diaminopimelic acid as diagnostic diamino acid and the peptidoglycan type was A3c. The peptidoglycan cell wall contained LL-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio of 1.5: 0.9: 1.0: 1.5. The cell-wall sugars were galactose and rhamnose. The predominant menaquinone was MK-9(H4). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid, glycolipid and four unknown phospholipids. The major cellular fatty acids were C18: 1ω9c (30.8 %), C16: 0 (24.1 %), and 10- methyl C18: 0 (13.9 %). The DNA G+C content was 72.0±0.1 mol%. On the basis of phylogenetic and phenotypic differences from members of the genera of the family Nocardioidaceae, a novel genus and species, Mumia flava gen. nov., sp. nov. are proposed. The type strain of Mumia flava is MUSC 201T (=DSM 27763T=MCCC 1A00646T=NBRC 109973T).

Original languageEnglish
Pages (from-to)1461-1467
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume64
Issue numberPART 5
DOIs
Publication statusPublished - 2014

Fingerprint

Nocardioidaceae
Peptidoglycan
Actinobacteria
Diaminopimelic Acid
rRNA Genes
Cell Wall
new species
acid
peptidoglycans
Vitamin K 2
Rhamnose
Phosphatidylglycerols
Cardiolipins
Glycolipids
Malaysia
Base Composition
Galactose
Alanine
Glycine
Marmoricola

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Mumia flava gen. nov., sp. nov., an actinobacterium of the family Nocardioidaceae. / Lee, Learn Han; Zainal, Nurullhudda; Azman, Adzzie Shazleen; Ab Mutalib, Nurul Syakima; Hong, Kui; Chan, Kok Gan.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 64, No. PART 5, 2014, p. 1461-1467.

Research output: Contribution to journalArticle

Lee, Learn Han ; Zainal, Nurullhudda ; Azman, Adzzie Shazleen ; Ab Mutalib, Nurul Syakima ; Hong, Kui ; Chan, Kok Gan. / Mumia flava gen. nov., sp. nov., an actinobacterium of the family Nocardioidaceae. In: International Journal of Systematic and Evolutionary Microbiology. 2014 ; Vol. 64, No. PART 5. pp. 1461-1467.
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abstract = "A novel actinobacterial strain, designated MUSC 201T, was isolated from a mangrove soil collected from Kuantan, the capital city of Pahang State in Malaysia. The taxonomic status of this strain was determined using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain MUSC 201T represented a novel lineage within the class Actinobacteria. Strain MUSC 201T formed a distinct clade in the family Nocardioidaceae and was most closely related to the members of the genera Nocardioides (16S rRNA gene sequence similarity, 91.9-95.1 {\%}), Aeromicrobium (92.7-94.6 {\%}), Marmoricola (92.5-93.1 {\%}) and Kribbella (91.5-92.4 {\%}). The cells of this strain were irregular coccoid to short rod shaped. The peptidoglycan contained LL-diaminopimelic acid as diagnostic diamino acid and the peptidoglycan type was A3c. The peptidoglycan cell wall contained LL-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio of 1.5: 0.9: 1.0: 1.5. The cell-wall sugars were galactose and rhamnose. The predominant menaquinone was MK-9(H4). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid, glycolipid and four unknown phospholipids. The major cellular fatty acids were C18: 1ω9c (30.8 {\%}), C16: 0 (24.1 {\%}), and 10- methyl C18: 0 (13.9 {\%}). The DNA G+C content was 72.0±0.1 mol{\%}. On the basis of phylogenetic and phenotypic differences from members of the genera of the family Nocardioidaceae, a novel genus and species, Mumia flava gen. nov., sp. nov. are proposed. The type strain of Mumia flava is MUSC 201T (=DSM 27763T=MCCC 1A00646T=NBRC 109973T).",
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AU - Hong, Kui

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N2 - A novel actinobacterial strain, designated MUSC 201T, was isolated from a mangrove soil collected from Kuantan, the capital city of Pahang State in Malaysia. The taxonomic status of this strain was determined using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain MUSC 201T represented a novel lineage within the class Actinobacteria. Strain MUSC 201T formed a distinct clade in the family Nocardioidaceae and was most closely related to the members of the genera Nocardioides (16S rRNA gene sequence similarity, 91.9-95.1 %), Aeromicrobium (92.7-94.6 %), Marmoricola (92.5-93.1 %) and Kribbella (91.5-92.4 %). The cells of this strain were irregular coccoid to short rod shaped. The peptidoglycan contained LL-diaminopimelic acid as diagnostic diamino acid and the peptidoglycan type was A3c. The peptidoglycan cell wall contained LL-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio of 1.5: 0.9: 1.0: 1.5. The cell-wall sugars were galactose and rhamnose. The predominant menaquinone was MK-9(H4). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid, glycolipid and four unknown phospholipids. The major cellular fatty acids were C18: 1ω9c (30.8 %), C16: 0 (24.1 %), and 10- methyl C18: 0 (13.9 %). The DNA G+C content was 72.0±0.1 mol%. On the basis of phylogenetic and phenotypic differences from members of the genera of the family Nocardioidaceae, a novel genus and species, Mumia flava gen. nov., sp. nov. are proposed. The type strain of Mumia flava is MUSC 201T (=DSM 27763T=MCCC 1A00646T=NBRC 109973T).

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