Multiplex PCR for detection of Helicobacter pylori infection in gastric biopsies with lower inflammatory score

Najmiyatul Fadilah, Alfizah Hanafiah, Hamizah Razlan, Zhiqin Wong, Isa Mohamed Rose, Md. Mostafizur Rahman

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: No gold standard has yet been established for the diagnosis of H. pylori infection. A multiplex polymerase chain reaction (mPCR) was developed in this study for rapid, sensitive and specific detection of H. pylori from gastric biopsies. Methods:H. pylori infections were determined by in-house rapid urease test (iRUT), culture, histology and multiplex PCR. Results: A total of 140 (60.9%) from 230 patients were positive for H. pylori infection. H. pylori were detected in 9.6% (22/230), 17% (39/230), 12.6% (29/230) and 60% (138/230) of biopsy specimens by culture, iRUT, histology and mPCR, respectively. mPCR identified H. pylori infection in 100% of biopsies with positive histology and culture. All biopsies with positive iRUT yielded positive PCR except two cases. mPCR also detected H. pylori in additional 116, 101 and 109 biopsies that were negative by culture, iRUT and histology, respectively. Positive samples by mPCR showed lower average in H. pylori density, activity and inflammation scores. The Indians showed the highest prevalence of H. pylori infection compared to the Chinese and the Malays. In addition, Chinese patients with older age were significantly infected compared to other ethnicities. Conclusion: PCR was able to detect the highest numbers of positive cases although the lowest average scores were recorded in the activity, inflammatory and H. pylori density.

Original languageEnglish
Pages (from-to)180-187
Number of pages8
JournalBritish Journal of Biomedical Science
Volume73
Issue number4
DOIs
Publication statusPublished - 1 Oct 2016

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Biopsy
Multiplex Polymerase Chain Reaction
Polymerase chain reaction
Helicobacter Infections
Histology
Helicobacter pylori
Urease
Stomach
Polymerase Chain Reaction
Inflammation

Keywords

  • diagnosis
  • Helicobacter pylori
  • inflammatory scores
  • multiplex PCR

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Medicine(all)
  • Clinical Biochemistry
  • Biochemistry, medical
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Multiplex PCR for detection of Helicobacter pylori infection in gastric biopsies with lower inflammatory score. / Fadilah, Najmiyatul; Hanafiah, Alfizah; Razlan, Hamizah; Wong, Zhiqin; Mohamed Rose, Isa; Rahman, Md. Mostafizur.

In: British Journal of Biomedical Science, Vol. 73, No. 4, 01.10.2016, p. 180-187.

Research output: Contribution to journalArticle

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