Multiple antibiotic resistance (Mar), plasmid profiles, and dna polymorphisms among vibrio vulnificus isolates

Mohammed M.Kurdi Al-Dulaimi, Sahilah Abd. Mutalib, Ma`Aruf Abd. Ghani, Nurul Aqilah Mohd Zaini, Ahmad Azuhairi Ariffin

Research output: Contribution to journalArticle

Abstract

Sixty strains (n = 60) of Vibrio vulnificus were examined for their multiple antibiotic resistance (MAR) index, plasmid profiles, and DNA polymorphisms. Thirty-seven strains (n = 37) were isolated from cockles (Anadara granosa) in Malaysia, while 23 (n = 23) isolates were isolated from clams (Mercenaria mercenaria) in Qatar. All isolates were resistant to two or more of the antibiotics tested, with the most common resistances were demonstrated towards penicillin (93%), ampicillin (70%), cephalothin (65%), clindamycin (66%), vancomycin (64%), and erythromycin (51%). The antibiotic that experienced the least resistance was kanamycin (6%), and all isolates were susceptible to cefoperazone, streptomycin, and tetracycline. The MAR index for the V. vulnificus isolated from Malaysia and Qatar, possessed similar values which ranged from 0.2 to 0.7, respectively. Plasmid analysis demonstrated that 65% of V. vulnificus strains harbored plasmids, while 35% were not. Nineteen (P1–P19) plasmids profiles were observed. No specific cluster or group was observed although they were isolated from different sample sources and locations by phylogenetic analysis using GelCompar II software at an 80% similarity level. Results demonstrated the high MAR index and genomic heterogeneity of V. vulnificus, which are of great concern to the human health of those who have consumed cockles and clams from the study area.

Original languageEnglish
Article number68
JournalAntibiotics
Volume8
Issue number2
DOIs
Publication statusPublished - 1 Jun 2019

Fingerprint

Vibrio vulnificus
Microbial Drug Resistance
Polymorphism
Mercenaria
Cardiidae
Plasmids
Qatar
Anti-Bacterial Agents
Bivalvia
Malaysia
Arcidae
Kanamycin Resistance
Cefoperazone
Cephalothin
Clindamycin
Streptomycin
Erythromycin
Vancomycin
Ampicillin
Tetracycline

Keywords

  • DNA polymorphisms
  • Genomic heterogeneity
  • Multiple antibiotic resistance (MAR) index
  • Plasmid profiles
  • Vibrio vulnificus

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

Cite this

Multiple antibiotic resistance (Mar), plasmid profiles, and dna polymorphisms among vibrio vulnificus isolates. / Al-Dulaimi, Mohammed M.Kurdi; Abd. Mutalib, Sahilah; Abd. Ghani, Ma`Aruf; Mohd Zaini, Nurul Aqilah; Ariffin, Ahmad Azuhairi.

In: Antibiotics, Vol. 8, No. 2, 68, 01.06.2019.

Research output: Contribution to journalArticle

@article{eb0b07c4c9b844ffad970ee66a7b45b2,
title = "Multiple antibiotic resistance (Mar), plasmid profiles, and dna polymorphisms among vibrio vulnificus isolates",
abstract = "Sixty strains (n = 60) of Vibrio vulnificus were examined for their multiple antibiotic resistance (MAR) index, plasmid profiles, and DNA polymorphisms. Thirty-seven strains (n = 37) were isolated from cockles (Anadara granosa) in Malaysia, while 23 (n = 23) isolates were isolated from clams (Mercenaria mercenaria) in Qatar. All isolates were resistant to two or more of the antibiotics tested, with the most common resistances were demonstrated towards penicillin (93{\%}), ampicillin (70{\%}), cephalothin (65{\%}), clindamycin (66{\%}), vancomycin (64{\%}), and erythromycin (51{\%}). The antibiotic that experienced the least resistance was kanamycin (6{\%}), and all isolates were susceptible to cefoperazone, streptomycin, and tetracycline. The MAR index for the V. vulnificus isolated from Malaysia and Qatar, possessed similar values which ranged from 0.2 to 0.7, respectively. Plasmid analysis demonstrated that 65{\%} of V. vulnificus strains harbored plasmids, while 35{\%} were not. Nineteen (P1–P19) plasmids profiles were observed. No specific cluster or group was observed although they were isolated from different sample sources and locations by phylogenetic analysis using GelCompar II software at an 80{\%} similarity level. Results demonstrated the high MAR index and genomic heterogeneity of V. vulnificus, which are of great concern to the human health of those who have consumed cockles and clams from the study area.",
keywords = "DNA polymorphisms, Genomic heterogeneity, Multiple antibiotic resistance (MAR) index, Plasmid profiles, Vibrio vulnificus",
author = "Al-Dulaimi, {Mohammed M.Kurdi} and {Abd. Mutalib}, Sahilah and {Abd. Ghani}, Ma`Aruf and {Mohd Zaini}, {Nurul Aqilah} and Ariffin, {Ahmad Azuhairi}",
year = "2019",
month = "6",
day = "1",
doi = "10.3390/antibiotics8020068",
language = "English",
volume = "8",
journal = "Antibiotics",
issn = "2079-6382",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "2",

}

TY - JOUR

T1 - Multiple antibiotic resistance (Mar), plasmid profiles, and dna polymorphisms among vibrio vulnificus isolates

AU - Al-Dulaimi, Mohammed M.Kurdi

AU - Abd. Mutalib, Sahilah

AU - Abd. Ghani, Ma`Aruf

AU - Mohd Zaini, Nurul Aqilah

AU - Ariffin, Ahmad Azuhairi

PY - 2019/6/1

Y1 - 2019/6/1

N2 - Sixty strains (n = 60) of Vibrio vulnificus were examined for their multiple antibiotic resistance (MAR) index, plasmid profiles, and DNA polymorphisms. Thirty-seven strains (n = 37) were isolated from cockles (Anadara granosa) in Malaysia, while 23 (n = 23) isolates were isolated from clams (Mercenaria mercenaria) in Qatar. All isolates were resistant to two or more of the antibiotics tested, with the most common resistances were demonstrated towards penicillin (93%), ampicillin (70%), cephalothin (65%), clindamycin (66%), vancomycin (64%), and erythromycin (51%). The antibiotic that experienced the least resistance was kanamycin (6%), and all isolates were susceptible to cefoperazone, streptomycin, and tetracycline. The MAR index for the V. vulnificus isolated from Malaysia and Qatar, possessed similar values which ranged from 0.2 to 0.7, respectively. Plasmid analysis demonstrated that 65% of V. vulnificus strains harbored plasmids, while 35% were not. Nineteen (P1–P19) plasmids profiles were observed. No specific cluster or group was observed although they were isolated from different sample sources and locations by phylogenetic analysis using GelCompar II software at an 80% similarity level. Results demonstrated the high MAR index and genomic heterogeneity of V. vulnificus, which are of great concern to the human health of those who have consumed cockles and clams from the study area.

AB - Sixty strains (n = 60) of Vibrio vulnificus were examined for their multiple antibiotic resistance (MAR) index, plasmid profiles, and DNA polymorphisms. Thirty-seven strains (n = 37) were isolated from cockles (Anadara granosa) in Malaysia, while 23 (n = 23) isolates were isolated from clams (Mercenaria mercenaria) in Qatar. All isolates were resistant to two or more of the antibiotics tested, with the most common resistances were demonstrated towards penicillin (93%), ampicillin (70%), cephalothin (65%), clindamycin (66%), vancomycin (64%), and erythromycin (51%). The antibiotic that experienced the least resistance was kanamycin (6%), and all isolates were susceptible to cefoperazone, streptomycin, and tetracycline. The MAR index for the V. vulnificus isolated from Malaysia and Qatar, possessed similar values which ranged from 0.2 to 0.7, respectively. Plasmid analysis demonstrated that 65% of V. vulnificus strains harbored plasmids, while 35% were not. Nineteen (P1–P19) plasmids profiles were observed. No specific cluster or group was observed although they were isolated from different sample sources and locations by phylogenetic analysis using GelCompar II software at an 80% similarity level. Results demonstrated the high MAR index and genomic heterogeneity of V. vulnificus, which are of great concern to the human health of those who have consumed cockles and clams from the study area.

KW - DNA polymorphisms

KW - Genomic heterogeneity

KW - Multiple antibiotic resistance (MAR) index

KW - Plasmid profiles

KW - Vibrio vulnificus

UR - http://www.scopus.com/inward/record.url?scp=85068454225&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85068454225&partnerID=8YFLogxK

U2 - 10.3390/antibiotics8020068

DO - 10.3390/antibiotics8020068

M3 - Article

AN - SCOPUS:85068454225

VL - 8

JO - Antibiotics

JF - Antibiotics

SN - 2079-6382

IS - 2

M1 - 68

ER -