Morphology and molecular phylogeny of the marine diatom Nitzschia dentatum sp. nov. and N. Johorensis sp. nov. (Bacillariophyceae) from Malaysia

S. N.P. Suriyanti, Gires Usup

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The marine diatom Nitzschia dentatum sp. nov. isolated from seawater samples of Kudat and N. johorensis sp. nov. isolated from beach sand samples of Sibu Island, Malaysia, have been described in this paper. Morphological identification, molecular phylogeny and toxin analyses were executed on the pure non-axenic algal cultures designated as KD89 and PS8, respectively. The main distinguishing feature of N. dentatum sp. nov. compared to other species is the jaggedcingulum structure which is only unique to this species. Meanwhile, N. johorensis sp. nov.is strongly characterized by the 'hantzschioid'and 'nitzschioid' symmetry dimorphisms; a common diagnostic feature but rarely described in other Nitzschia species. Identification of both strains was made based on the frustule diagnostic features and verified using the partial large ribosomal subunit DNA sequences. The results have confirmed that these two speciesare independent entities and novel species that have not been documented elsewhere. A notable finding from the Maximum Likelihood (ML), Maximum Parsimony (MP) and Bayesian Index (BI) analyses have also revealed that Nitzschia species that have indentation in the middle of valves have been consistently grouped as same clade with high bootstrap values. The extracts of both species did not show detectable amount of domoicacid and have therefore, been classified as non-toxic. This discovery contributes to the documentation of Nitzschia species worldwide.

Original languageEnglish
Pages (from-to)183-196
Number of pages14
JournalBangladesh Journal of Plant Taxonomy
Volume24
Issue number2
DOIs
Publication statusPublished - 1 Jan 2017

Fingerprint

Nitzschia
Bacillariophyceae
Malaysia
phylogeny
diatom
new species
dimorphism
beaches
toxins
seawater
algal culture
sand
indentation
nucleotide sequences
sampling
extracts
toxin
symmetry
beach
DNA

Keywords

  • Girdle
  • Hantzschiod
  • Indented valves
  • Jagged
  • New species
  • Pennate diatom

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

Cite this

Morphology and molecular phylogeny of the marine diatom Nitzschia dentatum sp. nov. and N. Johorensis sp. nov. (Bacillariophyceae) from Malaysia. / Suriyanti, S. N.P.; Usup, Gires.

In: Bangladesh Journal of Plant Taxonomy, Vol. 24, No. 2, 01.01.2017, p. 183-196.

Research output: Contribution to journalArticle

@article{79ac943a5d424cca89b6ec3e55f9e710,
title = "Morphology and molecular phylogeny of the marine diatom Nitzschia dentatum sp. nov. and N. Johorensis sp. nov. (Bacillariophyceae) from Malaysia",
abstract = "The marine diatom Nitzschia dentatum sp. nov. isolated from seawater samples of Kudat and N. johorensis sp. nov. isolated from beach sand samples of Sibu Island, Malaysia, have been described in this paper. Morphological identification, molecular phylogeny and toxin analyses were executed on the pure non-axenic algal cultures designated as KD89 and PS8, respectively. The main distinguishing feature of N. dentatum sp. nov. compared to other species is the jaggedcingulum structure which is only unique to this species. Meanwhile, N. johorensis sp. nov.is strongly characterized by the 'hantzschioid'and 'nitzschioid' symmetry dimorphisms; a common diagnostic feature but rarely described in other Nitzschia species. Identification of both strains was made based on the frustule diagnostic features and verified using the partial large ribosomal subunit DNA sequences. The results have confirmed that these two speciesare independent entities and novel species that have not been documented elsewhere. A notable finding from the Maximum Likelihood (ML), Maximum Parsimony (MP) and Bayesian Index (BI) analyses have also revealed that Nitzschia species that have indentation in the middle of valves have been consistently grouped as same clade with high bootstrap values. The extracts of both species did not show detectable amount of domoicacid and have therefore, been classified as non-toxic. This discovery contributes to the documentation of Nitzschia species worldwide.",
keywords = "Girdle, Hantzschiod, Indented valves, Jagged, New species, Pennate diatom",
author = "Suriyanti, {S. N.P.} and Gires Usup",
year = "2017",
month = "1",
day = "1",
doi = "10.3329/bjpt.v24i2.35114",
language = "English",
volume = "24",
pages = "183--196",
journal = "Bangladesh Journal of Plant Taxonomy",
issn = "1028-2092",
publisher = "Bangladesh Association of Plant Taxonomists (BAPT)",
number = "2",

}

TY - JOUR

T1 - Morphology and molecular phylogeny of the marine diatom Nitzschia dentatum sp. nov. and N. Johorensis sp. nov. (Bacillariophyceae) from Malaysia

AU - Suriyanti, S. N.P.

AU - Usup, Gires

PY - 2017/1/1

Y1 - 2017/1/1

N2 - The marine diatom Nitzschia dentatum sp. nov. isolated from seawater samples of Kudat and N. johorensis sp. nov. isolated from beach sand samples of Sibu Island, Malaysia, have been described in this paper. Morphological identification, molecular phylogeny and toxin analyses were executed on the pure non-axenic algal cultures designated as KD89 and PS8, respectively. The main distinguishing feature of N. dentatum sp. nov. compared to other species is the jaggedcingulum structure which is only unique to this species. Meanwhile, N. johorensis sp. nov.is strongly characterized by the 'hantzschioid'and 'nitzschioid' symmetry dimorphisms; a common diagnostic feature but rarely described in other Nitzschia species. Identification of both strains was made based on the frustule diagnostic features and verified using the partial large ribosomal subunit DNA sequences. The results have confirmed that these two speciesare independent entities and novel species that have not been documented elsewhere. A notable finding from the Maximum Likelihood (ML), Maximum Parsimony (MP) and Bayesian Index (BI) analyses have also revealed that Nitzschia species that have indentation in the middle of valves have been consistently grouped as same clade with high bootstrap values. The extracts of both species did not show detectable amount of domoicacid and have therefore, been classified as non-toxic. This discovery contributes to the documentation of Nitzschia species worldwide.

AB - The marine diatom Nitzschia dentatum sp. nov. isolated from seawater samples of Kudat and N. johorensis sp. nov. isolated from beach sand samples of Sibu Island, Malaysia, have been described in this paper. Morphological identification, molecular phylogeny and toxin analyses were executed on the pure non-axenic algal cultures designated as KD89 and PS8, respectively. The main distinguishing feature of N. dentatum sp. nov. compared to other species is the jaggedcingulum structure which is only unique to this species. Meanwhile, N. johorensis sp. nov.is strongly characterized by the 'hantzschioid'and 'nitzschioid' symmetry dimorphisms; a common diagnostic feature but rarely described in other Nitzschia species. Identification of both strains was made based on the frustule diagnostic features and verified using the partial large ribosomal subunit DNA sequences. The results have confirmed that these two speciesare independent entities and novel species that have not been documented elsewhere. A notable finding from the Maximum Likelihood (ML), Maximum Parsimony (MP) and Bayesian Index (BI) analyses have also revealed that Nitzschia species that have indentation in the middle of valves have been consistently grouped as same clade with high bootstrap values. The extracts of both species did not show detectable amount of domoicacid and have therefore, been classified as non-toxic. This discovery contributes to the documentation of Nitzschia species worldwide.

KW - Girdle

KW - Hantzschiod

KW - Indented valves

KW - Jagged

KW - New species

KW - Pennate diatom

UR - http://www.scopus.com/inward/record.url?scp=85039865819&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85039865819&partnerID=8YFLogxK

U2 - 10.3329/bjpt.v24i2.35114

DO - 10.3329/bjpt.v24i2.35114

M3 - Article

AN - SCOPUS:85039865819

VL - 24

SP - 183

EP - 196

JO - Bangladesh Journal of Plant Taxonomy

JF - Bangladesh Journal of Plant Taxonomy

SN - 1028-2092

IS - 2

ER -